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Magnetic beads–assisted fluorescence aptasensing approach based on dual DNA tweezers for detection of ochratoxin A and fumonisin B1 in wine and corn

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Abstract

A magnetic beads (MBs)–assisted fluorescence aptasensing approach based on dual DNA tweezers and magnetic separation was established for the detection of ochratoxin A (OTA) and fumonisin B1 (FB1). A dual DNA tweezers structure with four ends linked with fluorophores (FAM, ROX) and quenchers (BHQ1, BHQ2) was designed, and produced the high initial fluorescence signals because of the long spatial distance between FAM and BHQ1, ROX, and BHQ2. Bio-aptamer/anti-aptamer of OTA and bio-aptamer/anti-aptamer of FB1 were respectively annealed to form dsDNA, and immobilized to MBs coated with streptavidin (SA). With the existence of OTA and FB1, OTA and FB1 preferentially bound with their respective bio-aptamers, which made anti-aptamers dissociate from dsDNA coupled on MBs. After magnetic separation, the dissociated anti-aptamers reacted with dual DNA tweezers, respectively, which made DNA tweezers close and the fluorescence was quenched. The linear ranges of approach for OTA and FB1 detection were 0.05–20 ng/mL and 0.1–40 ng/mL, respectively. The limit of detection for OTA and FB1 was 0.029 ng/mL and 0.061 ng/mL. The prepared MBs-assisted fluorescence aptasensing approach was applied to detect OTA and FB1 in spiked red wine and corn samples, which showed good recoveries between 92 and 106%.

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Funding

This study was funded by the Key Scientific and Technological Project of Henan Province (212102310001) and the Innovative Funds Plan of Henan University of Technology (2020ZKCJ14).

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Authors and Affiliations

  1. College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, No. 100 Lianhua Street, Zhengzhou, 450001, Henan, People’s Republic of China

    Chenling Qu, Luyang Zhao, Xing He & Min Wei

  2. College of Public Health, Zhengzhou University, Zhengzhou, 450001, People’s Republic of China

    Songcheng Yu

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  1. Chenling Qu
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Qu, C., Zhao, L., He, X. et al. Magnetic beads–assisted fluorescence aptasensing approach based on dual DNA tweezers for detection of ochratoxin A and fumonisin B1 in wine and corn. Anal Bioanal Chem 413, 6677–6685 (2021). https://doi.org/10.1007/s00216-021-03635-7

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