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Singlet oxygen-generating from fluorescence probes based on denatured bovine serum albumin-conjugated CdTe quantum dots and photosensitizer Chlorin e6

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Abstract

Based on the excellent optical properties of quantum dots (QDs), a simple and efficient method was proposed and established to produce a singlet oxygen activating QDs-denatured Bovine Serum Albumin (dBSA)-Chlorin e6 (Ce6) bioprobe. In this work, a quick synthesis of water-soluble CdTe QDs was accomplished and showed a good linear fit between synthetic heating duration and its maximum fluorescence emission wavelength. Moreover, dBSA which is a convenient and widely used agent was employed as a bridge to conjugate the selective CdTe QDs with a suitable emitting wavelength and photosensitizer Ce6, simply using N-ethyl-N-(3-dimethylaminopropyl) carbodiimide and N-hydroxy succinimide as coupling agents. UV–Vis, fluorescence, and IR spectroscopy confirmed the successful conjugation of QDs and Ce6. The as-produced QDs-dBSA-Ce6 bioconjugates demonstrated an effective fluorescence resonance energy transfer efficiency of as high as 0.90, which also proved to be well conserved of its singlet oxygen-generating in oxidation–extraction photometry tests and might be a potential bioprobe in the research of photo dynamic therapy.

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Acknowledgments

The authors are grateful to Fundamental Research Funds for the Central Universities (Program No: 02410415) and Jiangsu Youth Fund (Program No: BK20140661) for their financial support.

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Authors and Affiliations

  1. Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 211198, China

    Sheng-Mei Wu, Xiao-Juan Sun, Li-Li Wang, Meng-Ying Fei & Zheng-Yu Yan

  2. Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing, 211198, China

    Sheng-Mei Wu, Xiao-Juan Sun, Li-Li Wang, Meng-Ying Fei & Zheng-Yu Yan

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  1. Sheng-Mei Wu
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  2. Xiao-Juan Sun
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  3. Li-Li Wang
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Correspondence to Zheng-Yu Yan.

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Wu, SM., Sun, XJ., Wang, LL. et al. Singlet oxygen-generating from fluorescence probes based on denatured bovine serum albumin-conjugated CdTe quantum dots and photosensitizer Chlorin e6. J Nanopart Res 16, 2701 (2014). https://doi.org/10.1007/s11051-014-2701-y

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