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Preparation of Different Lights Irradiated ZnSe/ GSH QDs and Their Interaction with BSA

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Abstract

Using L-glutathione (GSH) as a capping agent, ZnSe/GSH quantum dots (QDs) were prepared under microwave irradiation and irradiated under dark, ultraviolet light and incandescent light, respectively. The properties and interaction of different lights irradiated ZnSe/GSH QDs and bovine serum albumin (BSA) were studied systematically. The fluorescence (FL) spectra results reveal that the quenching mechanism are all the static quenching in nature. The quenching constant (Ksv) and binding constant (K) value of different irradiated ZnSe/GSH QDs and BSA all increased with the change of light types from dark to incandescent light and UV light. The number of binding site (n) is close to 1 at different temperatures. The lighting types influence the enthalpy and entropy changes. The Fourier transform infrared (FTIR) spectra indicate that the light induced GSH ligand will facilitate photocatalytic oxidation on the surface of ZnSe/GSH QDs. The circular dichroism (CD) results show that the α-helicity content of BSA decreases from 60.34%, 59.31%, to 58.79% under UV lighting, incandescent lighting and dark conditions. The interaction results of different lights illuminated ZnSe/GSH QDs with BSA by CD spectra method matches well with that by FL and FTIR spectra. That is, the interaction of ZnSe/ GSH QDs and BSA from strong to weak is UV light, incandescent light and dark in sequence.

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Authors and Affiliations

  1. The State Key Laboratory of Refractories and Metallurgy, Wuhan University of Science and Technology, Wuhan, 430081, China

    Ling Ding  (丁玲) & Huan He

  2. Coal Conversion and New Carbon Materials Hubei Key Laboratory, School of Chnemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan, 430081, China

    Ling Ding  (丁玲), Huan He, Jin Zhou, Kuntao Huang, Zhengzai Cheng & Mario Gauthier

  3. Key Laboratory of Measurement and Control System for Offshore Environment, Fuqing Branch of Fujian Normal University, Fuqing, 350300, China

    Ling Ding  (丁玲) & Shiqian Li  (李世迁)

Authors
  1. Ling Ding  (丁玲)
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  2. Huan He
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  3. Shiqian Li  (李世迁)
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  4. Jin Zhou
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  5. Kuntao Huang
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  6. Zhengzai Cheng
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  7. Mario Gauthier
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Correspondence to Ling Ding  (丁玲) or Shiqian Li  (李世迁).

Additional information

Funded by the Natural Science Foundation of Hubei Province (2017CFB680), the Open Fund of the State Key Laboratory of Refractories and Metallurgy Wuhan University of Science and Technology (G201703), the Fund of Key Laboratory of Measurement and Control System for Offshore Environment, Fuqing Branch of Fujian Normal University (SI-KF1604), the Guiding Projects in Fujian Province (2018H0013), and the Key Natural Fund Projects of Universities in Fujian Province (JZ160490)

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Ding, L., He, H., Li, S. et al. Preparation of Different Lights Irradiated ZnSe/ GSH QDs and Their Interaction with BSA. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 34, 858–865 (2019). https://doi.org/10.1007/s11595-019-2129-4

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  • DOI: https://doi.org/10.1007/s11595-019-2129-4

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