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CRISPR/Cas9 is a powerful tool for precise genome editing of legume crops: a review

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Abstract

Legumes are an imperative source of food and proteins across the globe. They also improve soil fertility through symbiotic nitrogen fixation (SNF). Genome editing (GE) is now a novel way of developing desirable traits in legume crops. Genome editing tools like clustered regularly interspaced short palindromic repeats (CRISPR) system permits a defined genome alteration to improve crop performance. This genome editing tool is reliable, cost-effective, and versatile, and it has to deepen in terms of use compared to other tools. Recently, many novel variations have drawn the attention of plant geneticists, and efforts are being made to develop trans-gene-free cultivars for ensuring biosafety measures. This review critically elaborates on the recent development in genome editing of major legumes crops. We hope this updated review will provide essential informations for the researchers working on legumes genome editing. In general, the CRISPR/Cas9 novel GE technique can be integrated with other techniques like omics approaches and next-generation tools to broaden the range of gene editing and develop any desired legumes traits. Regulatory ethics of CRISPR/Cas9 are also discussed.

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Acknowledgements

The authors would also like to thank the Deanship of Scientific Research at Umm Al-Qura University for contributing support via grant code (19-SCI-1-01-0009).

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Authors and Affiliations

  1. Key Laboratory of Crops Physiology, Ecology and Genetic Breeding, Ministry of Education/College of Agronomy, Jiangxi Agricultural University, 330045, Nanchang, China

    Adnan Rasheed

  2. Department of Chemistry, Al-Leith University College, Umm Al Qura University, Makkah, Saudi Arabia

    Aminah A. Barqawi

  3. Department of Agronomy, University of Agriculture Faisalabad, 38040, Faisalabad, Pakistan

    Athar Mahmood

  4. Department of Agricultural Engineering, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan, 64200, Punjab, Pakistan

    Muhammad Nawaz & Adnan Noor Shah

  5. Department of Biology, Faculty of Applied Sciences, Umm Al-Qura University, Makkah, Saudi Arabia

    Daniyah H. Bay

  6. Biology Department Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia

    Maryam A. Alahdal

  7. Research Center on Ecological Sciences, Jiangxi Agricultural University, 330045, Nanchang, China

    Muhammad Umair Hassan

  8. Department of Biology, Al-Jumum University College, Umm Al-Qura University, 21955, Makkah, Saudi Arabia

    Sameer H. Qari

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Contributions

Acknowledgement:?Conceptualization, writing, and original draft preparation, AR., MUH., MN.; review editing and Scientific figures improvement , ANS., ?AAB., AM., DHB., MAA.; Supervision and funding, SHQ.

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Correspondence to Adnan Noor Shah or Sameer H. Qari.

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Rasheed, A., Barqawi, A.A., Mahmood, A. et al. CRISPR/Cas9 is a powerful tool for precise genome editing of legume crops: a review. Mol Biol Rep 49, 5595–5609 (2022). https://doi.org/10.1007/s11033-022-07529-4

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