Skip to main content
SpringerLink
Log in

Genetic structure of Alhagi (Hedysareae, Fabaceae) populations using ISSR data in Iran

  • Original Article
  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Alhagi Gagnebin (Fabaceae: Hedysareae) is a small genus of shrubs or subshrubs distributed in temperate and tropical regions of Asia, Europe, and Africa. The exact number of Alhagi species in Iran has been disputable. Studies showed that morphology, seed protein, and chromosome characters could not clearly delimitate the species boundaries of the genus. In order to determine species boundaries and the population structure of Alhagi species in Iran, eight labeled inter simple sequence repeat (ISSR) primers were used to screen 22 populations including 110 individuals. STRUCTURE, PCoA and clustering analyses of ISSR data were able to clearly split all populations of Alhagi in Iran into two distinct groups. As the populations A. pseudalhagi and A. maurorum jointly formed a group (A. maurorum complex) segregated from those of Alhagi graecorum. Further analyses of A. maurorum complex showed a significant molecular difference among the studied populations (PhiPT value = 0.213, P = 0.001) as well as a high amount of variability within populations (79%) indicating frequent genetic exchanges. Structure analysis of complex populations (K = 2) showed no distinct genetic pattern related to their geographical distribution. In this study, Alhagi has retrieved the only two species of Alhagi in Iran; A. graecorum and A. maurorum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Legume Phylogeny Working Group (2013) Legume phylogeny and classification in the 21st century: progress, prospects and lessons for other species-rich clades. Taxon 62(2):217–248. https://doi.org/10.12705/622.8

    Article  Google Scholar 

  2. Stevens PF (2017) Angiosperm Phylogeny Website, version 14. Available at http://www.mobot.org/MOBOT/research/APweb. Accessed 20 Aug 2020

  3. Awmack C, Lock J (2002) The genus Alhagi (Leguminosae: Papilionoideae) in the Middle East. Kew Bull 57(2):435–443. https://doi.org/10.2307/4111121

    Article  Google Scholar 

  4. Xu L, Larsen K (2010) Alhagi Gagnebin. In: Wu Z, Raven P, Hong D (eds) Flora of China, vol. 10 (Fabaceae). Missouri Botanical Garden Press, Beijing, St. Louis, pp 526–527

    Google Scholar 

  5. Meikle RD (1977) Flora of Cyprus, vol. 1. Royal Botanic Gardens, Kew, London.

  6. Zahran M, Willis A (1992) The vegetation of Egypt. Springer, Netherlands

    Book  Google Scholar 

  7. Sheidai M, Yazdanbakhsh Z, Assadi M, Moussavi M (2001) Cytology and morphometry study of Alhagi (Leguminosae) species in Iran. Nord J Bot 21(1):83–91. https://doi.org/10.1111/j.1756-1051.2001.tb01341.x

    Article  Google Scholar 

  8. Ahangarian S, Kazem POS, Maassoumi AA (2007) Molecular phylogeny of the tribe Hedysareae with special reference to Onobrychis (Fabaceae) as inferred from nrDNA ITS sequences. Iran J Bot 13(2):64–74

    Google Scholar 

  9. Amirahmadi A, Kazempour-Osaloo S, Moein F, Kaveh A, Maassoumi AA (2014) Molecular systematics of the tribe Hedysareae (Fabaceae) based on nrDNA ITS and plastid trnL-F and matK sequences. Plant Syst Evol 300(4):729–747. https://doi.org/10.1007/s00606-013-0916-5

    Article  CAS  Google Scholar 

  10. Duan L, Wen J, Yang X, Liu PL, Arslan E, Ertuğrul K, Chang ZY (2015) Phylogeny of Hedysarum and tribe Hedysareae (Leguminosae: Papilionoideae) inferred from sequence data of ITS, matK, trnL-F and psbA-trnH. Taxon 64(1):49–64. https://doi.org/10.12705/641.26

    Article  Google Scholar 

  11. Liu P-L, Wen J, Duan L, Arslan E, Ertuğrul K, Chang Z-Y (2017) Hedysarum L. (Fabaceae: Hedysareae) is not monophyletic-evidence from phylogenetic analyses based on five nuclear and five plastid sequences. PLoS ONE 12(1):e0170596. https://doi.org/10.1371/journal.pone.0170596

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Chamberlain DF (1970) Alhagi Gagnebin. In: Davis PH (ed) Flora of Turkey and the East Aegean Islands, vol 3. Edinburgh University Press, Edinburgh, pp 596–597

    Google Scholar 

  13. Ali SI (1977) Alhagi Gagnebin. In: Nasir E, Ali SI (eds) Flora of West Pakistan, no. 100: Papilionaceae. University of Karachi, Karachi, pp 319–320

    Google Scholar 

  14. Rechinger KH (1984) Alhagi. In: Rechinger KH (ed) Flora Iranica, no. 157: Papilionaceae. Akademische Druck-und Verlagsanstalt, Graz, pp 470–475

    Google Scholar 

  15. Wu Z, Raven P, Hong D (2010) Flora of China, vol. 10: Fabaceae. Beijing and Missouri Botanical Garden Press, St. Louis

    Google Scholar 

  16. Sheidai M, Yazdanbakhsh Z, Bernard F (2002) Seed protein analysis of Alhagi (Leguminosae) species and populations. Iran J Bot 9(2):141–149

    Google Scholar 

  17. Mabberly DJ (2017) Mabberly’s plant-book: a portable dictionary of plants, their classification and uses, 4th edn. Cambridge University Press, Cambridge

    Book  Google Scholar 

  18. Amirkhosravi A, Asri Y, Assadi M, Mehregan I (2020) Systematics of Alhagi: molecular phylogeny and morphology revisited. Rostaniha 21(2):174–184. https://doi.org/10.22092/botany.2020.343028.1200

    Article  Google Scholar 

  19. Freeland JR (2005) Molecular ecology. Wiley, West Sussex

    Google Scholar 

  20. Godwin ID, Aitken EAB, Smith LW (1997) Application of inter-simple sequence repeat (ISSR) markers to plant genetics. Electrophoresis 18(9):1524–1528. https://doi.org/10.1002/elps.1150180906

    Article  CAS  PubMed  Google Scholar 

  21. Wolfe AD, Liston A (1998) Contributions of PCR-based methods to plant systematics and evolutionary biology. In: Soltis DE, Soltis PS, Doyle JJ (eds) Molecular systematics of plants II. Springer, Boston, pp 43–86. https://doi.org/10.1007/978-1-4615-5419-6_2

    Chapter  Google Scholar 

  22. Pradeep-Reddy M, Sarla N, Siddiq E (2002) Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128(1):9–17. https://doi.org/10.1023/A:1020691618797

    Article  CAS  Google Scholar 

  23. Ng W, Tan S (2015) Inter-simple sequence repeat (ISSR) markers: are we doing it right. ASM Sci J 9(1):30–39

    Google Scholar 

  24. Abate T (2017) Inter simple sequence repeat (ISSR) markers for genetic diversity studies in Trifolium Species. Adv Life Sci Technol 55:33–37

    Google Scholar 

  25. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19(1):11–15

    Google Scholar 

  26. Doyle J (1991) DNA protocols for plants. In: Hewitt GM, Johnston AWB, Young JPW (eds) Molecular techniques in taxonomy. NATO ASI series (series H: cell biology), vol 57. Springer. Berlin, Heidelberg, pp 283–293

    Chapter  Google Scholar 

  27. El-Hady E, Haiba AA, El-Hamid NRA, Al-Ansary A, Mohamed AY (2010) Assessment of genetic variations in some Vigna species by RAPD and ISSR analysis. N Y Sci J 3(11):120–128

    Google Scholar 

  28. Agarwal T, Gupta AK, Patel AK, Shekhawat N (2015) Micropropagation and validation of genetic homogeneity of Alhagi maurorum using SCoT, ISSR and RAPD markers. Plant Cell Tissue Org Cult 120:313–323. https://doi.org/10.1007/s11240-014-0608-z

    Article  CAS  Google Scholar 

  29. Hammer Ø (2012) PAST: paleontological statistics version 2.17, reference manual. Natural History Museum, University of Oslo, Oslo

    Google Scholar 

  30. Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6(1):288–295. https://doi.org/10.1111/j.1471-8286.2005.01155.x

    Article  Google Scholar 

  31. Nei M (1972) Genetic distance between populations. Am Nat 106(9):283–292. https://doi.org/10.1086/282771

    Article  Google Scholar 

  32. Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70(12 Pt 1–2):3321–3323. https://doi.org/10.1073/pnas.70.12.3321

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Yeh FC, Yang RC, Boyle T (1999) POPGENE version 1.31. Microsoft window-based freeware for population genetic analysis. University of Alberta, Edmonton

    Google Scholar 

  34. Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155(2):945–959

    Article  CAS  Google Scholar 

  35. Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software structure: a simulation study. Mol Ecol 14(8):2611–2620. https://doi.org/10.1111/j.1365-294X.2005.02553.x

    Article  CAS  Google Scholar 

  36. Yakovelov GP (1979) Notes on the taxonomy of the genus Alhagi Gagneb (Fabaceae). Bot Zhurnal 64(1):1794–1799

    Google Scholar 

  37. Lock JM, Simpson K (1991) Legume of the West Asia: a check-list. Royal Botanical Gardens, Kew, London

    Google Scholar 

  38. Matin-Fard MM, Bazubandi M, Karimi-Shahri MR (2010) Investigation of genetic diversity in Alhaji Pseudoalhaji using RAPD-PCR molecular markers. Proceeding of the 3rd Iranian Weed Science Congress, vol. 2, pp 630–634. February 2010, Babolsar. Available at https://www.cabdirect.org/cabdirect/abstract/20103165335. Accessed 17–18 Feb 2010

  39. Abd El-Hak GhM, Ibrahim Sh, Halem A, Hassan ZH, Rayan WA (2019) Assessment of genetic diversity in different geographically isolated Alhagi graecorum Boiss. populations using SCoT marker. Taeckholmia 39(1):44–66. https://doi.org/10.21608/taec.2019.19481.1008

    Article  Google Scholar 

  40. Boissier E (1872) Flora Orientalis: sive, Enumeratio plantarum in Oriente a Graecia et Aegypto ad Indiae fines hucusque observatarum 2. Genevae, Basileae & Lugduni: H. Georg

Download references

Acknowledgements

The authors would like to thank the staff at IAUH and TARI herbaria for their help.

Funding

This work was supported by the Islamic Azad University, Science and Research Branch, Tehran, Iran.

Author information

Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Arezoo Amirkhosravi & Iraj Mehregan

  2. Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

    Younes Asri & Mostafa Assadi

Authors
  1. Arezoo Amirkhosravi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Younes Asri
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mostafa Assadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Iraj Mehregan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Younes Asri.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Amirkhosravi, A., Asri, Y., Assadi, M. et al. Genetic structure of Alhagi (Hedysareae, Fabaceae) populations using ISSR data in Iran. Mol Biol Rep 48, 5143–5150 (2021). https://doi.org/10.1007/s11033-021-06511-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-021-06511-w

Keywords

Search

Navigation