Abstract
Chronic oxidative stress has been associated with several human ailments including the condition of aging. Extensive studies have shown the causal relationship between oxidative stress, aging, and cellular senescence. In this regard, forestalling or preventing senescence could delay the aging process as well as act as an intervention against premature aging. Hence, in the present study, we investigated the anti-senescence potential of Mangiferin (MGN) against Hydrogen peroxide (H2O2) induced premature senescence using human dermal fibroblast cells. Early passage human dermal fibroblasts cells were exposed to H2O2 (10 μM) for 15 days. In order to assess the anti-senescence property of MGN, cells were preconditioned with MGN (10 μM / 50 μM; 2 h) followed by addition of H2O2 (10 μM). H2O2 mediated induction of premature senescence was accompanied by elevated ROS, lowering of mitochondrial mass and membrane potential, changes in ATP content along with G0/G1 arrest and SA-β-gal expression. While, conditioning the cells with MGN lowered oxidative burden, stabilized mitochondrial membrane potential / mass and protected the cells against cell cycle arrest, ultimately rendering protection against premature senescence. The present findings showed that MGN might act as a potential cytoprotective nutraceutical that can prolong the onset of chronic oxidative stress mediated premature senescence.
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Acknowledgements
The authors are indebted to Manipal School of Life Sciences, MAHE for providing the infrastructure and laboratory facilities. The authors are thankful to Dr. K. Satyamoorthy, Director, Manipal School of Life Sciences, MAHE for his support and encouragement.
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The above study utilized early passage primary human dermal fibroblast cells that were established from skin samples obtained from healthy donors with the prior approval from the institutional ethical clearance (approval number IEC 011/2009).
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Kanoi, R., Loachan, P., Das, S. et al. Mangiferin, a naturally occurring polyphenol, mitigates oxidative stress induced premature senescence in human dermal fibroblast cells. Mol Biol Rep 48, 457–466 (2021). https://doi.org/10.1007/s11033-020-06074-2
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DOI: https://doi.org/10.1007/s11033-020-06074-2