Abstract
Bitter (Ferula pseudalliacea) and sweet (Ferula assa-foetida) asafetida (Apiaceae family) are well-known economic and medicinal herbs owing to their gum. This study investigates genetic differentiation of F. pseudalliacea and F. assa-foetida using ISSR markers, to determine the effective primer and to assess the possibility of separating sweet and bitter plant populations from each other. Results showed that among 22 primers, eight markers reproduced obvious DNA patterns and revealed 234 scorable DNA bands. ISSR-16 and ISSR-55 primers had better performance than other primers according to the number of bands, PIC and Marker Index. Bitter population showed polymorphic loci (224), percentage of polymorphic loci (95.73%) and observed number of alleles (1.96 ± 0.2), while sweet populations showed the amount of these parameters as 218, 93.16% and 1.93 ± 0.25, respectively. Estimated Gst of sweet population was 0.09 and Gst of bitter population was 0.06. Comparing gene flow in bitter and sweet populations showed a lower level of gene flow between sweet populations (Nm = 4.93) compared to bitter ones (Nm = 7.89). Within group genetic similarity of sweet asafetida population was higher than between group variation of bitter and sweet populations. The highest similarity was observed between bitter populations (0.95). The highest genetic dissimilarity was also estimated between bitter and sweet populations (0.08). Cluster analysis grouped four studied populations into 13 clusters using Jaccardʼs similarity coefficient and UPGMA method. Principal coordinate analysis showed that 61.02% of total variance was explained using three components and it could completely separate populations as well as cluster analysis. These grouping correspond nearly with geographical distribution. Analysis of molecular variance showed that genetic variation within populations (87%) was more than among populations (13%). The results indicated that ISSR marker is suitable to investigate genetic diversity of asafetida populations and could separate populations of the same genera with similar germplasm.
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References
Ai B, Kang M, Huang H (2014) Assessment of genetic diversity in seed plants based on a uniform π criterion. Molecules 19:20113–20127
Akbari M, Salehi H, Niazi A (2018) Evaluation of diversity based on morphological variabilities and ISSR molecular markers in Iranian Cynodon dactylon (L.) Pers. accessions to select and introduce cold-tolerant genotypes. Mol Biotechnol 60(4):259–270
Akbarian A, Rahimmalek M, Sabzalian MR (2017) Variation in fruit morphological traits and bioactive compounds in different populations of Ferula assa-foetida, F. gummosa and F. ovina collected from Iran. J Agric Sci Technol 19:425–438
Alansi S, Tarroum M, Al-Qurainy F, Khan S, Nadeem M (2016) Use of ISSR markers to assess the genetic diversity in wild medicinal Ziziphus spina-christi. (L.) Willd. collected from different regions of Saudi Arabia. Biotechnol Biotechnol Equipment 30(5):942–947
Amiri MS, Joharchi MR (2016) Ethnobotanical knowledge of Apiaceae family in Iran: a review. Avicenna J Phytomed 6(6):621–635
Banerjee R, Chattopadhyay S, Saha AK (2016) Genetic diversity and relationship of mulberry genotypes revealed by RAPD and ISSR markers. J Crop Improv 4(30):478–492
Barrandeguy ME, Garcia MV (2014) Quantifying genetic diversity: the starting point for population genetic studies using molecular markers. J Genet 93(2):587–589
Bublyk OM, Andreev IO, Kalendar RN, Spiridonova KV, Kunakh VA (2013) Efficiency of different PCR-based marker systems for assessment of Iris pumila genetic diversity. Biologia 68(4):613–620
Canovas JL, Jimenez JF, Mota JF, Gomez PS (2015) Genetic diversity of Viola cazorlensis Gand., an endemic species of Mediterranean dolomitic habitats: implications for conservation. Syst Biodivers 13(6):571–580
Dettori CA, Sergi S, Tamburini E, Bacchetta G (2013) The genetic diversity and spatial genetic structure of the Corso-Sardinian endemic Ferula arrigonii Bocchieri (Apiaceae). Plant Biol 16:1005–1013
Drew DP, Dueholm B, Weitzel C, Zhang Y, Sensen CW, Simonsen HT (2013) Transcriptom analysis of Thapsia laciniata Rouy provides insight into terpenoid biosynthesis and diversity in Apiaceae. Int J Mol Sci 14:9080–9098
Fernandez-Mazuecos M, Jimenez-Mejias P, Rotllan-Puig X, Vargas P (2014) Narrow endemics to Mediterranean islands: moderate genetic diversity but narrow climatic niche of the ancient, critically endangered Naufraga (Apiaceae). Perspect Plant Ecol Evol Syst 16:190–202
Ghasemi Ghehsareh M, Salehi H, Khosh-Khui M, Niazi A (2015) Application of ISSR markers to analyze molecular relationships in Iranian Jasmine (Jasminum spp.). Mol Biotechnol 57(1):65–74
Hao B, Li W, Linchun M, Li Y, Rui Zh, Mingxia T, Weikai B (2006) A study of conservation genetics in Cupressus chengiana, an endangered endemic of China, using ISSR markers. Biochem Genet 44:29–43
Jia XD, Wang T, Zhai M, Li YR, Guo ZR (2011) Genetic diversity and identification of Chinese-grown pecan using ISSR and SSR markers. Molecules 16:10078–10092
Kameli Z, Alizadeh A, Aboutalebi A (2012) Bitter and sweet Ferula assa-foetida L. essential oil composition from Iran and its comparison with Ferula assa-foetida L. used in India. Int Res J Appl Basic Sci 3(5):919–923
Khederzadeh S, Samiei M, Mobaraki A, Ezeddinloo L, Haghi HA (2017) Genetic comparison of Iranian Asafetida (Ferula assa-foetida L.) populations based on cpDNA ribosomal protein L16 intron. Int J Agric Innov Res 5(4):577–583
Khoonani Z, Naghavi MR, OMidi M, Sabokdast M, Talebi Kooyakhi E (2012) Investigating genetic diversity of Ferula gummosa ecotypes in different regions of Iran using AFLP marker. J Med Plants 38(2):117–126
Lopez-Pujol J, Martinell MC, Masso S, Rovira AM, Bosch M, Molero J, Simon J, Blanche C (2013) Conservation genetics of Dichoropetalum schottii (Apiaceae): is the legal protection of edge populations consistent with the genetic data? Ann Botanici Fennici 50:269–283
Madani ZS, Fotookian MH, Karimi J, Talei D (2015) Genetic diversity lemon balm (Mellissa officinalis L.) geographical groups using ISSR markers. 1st International and 9th National Biotechnology Congress of Islamic Republic of Iran. Shahid Beheshti University, Tehran, pp 1–5
Melito S, Bella SL, Martinelli F, Cammalleri I, Tuttolomondo T, Leto C, Fadda A, Molinu MG, Mulas M (2016) Morphological, chemical and genetic diversity of wild myrtle (Myrtus communis L.) populations in Sicily. Turk J Agric For 40:249–261
Mohseni Z, Bernousi I, Mandoulakani BA, Darvishzadeh R (2015) Genetic diversity in Papaver bracteatum and Papaver somniferum populations revealed by ISSR markers. Bulgarian Journal of Agricultural Science 21(3):485–493
Mozaffarian V (2007) Flora of Iran, Umbeliferae. Research Institute of Forests and Rangelands Publications, Tehran
Naghavi MR, Gharayazi B, Hosseini Salekdeh Gh (2007) Molecular markers. Tehran University Publication Institute, Tehran
Nagy S, Poczai P, Cernak I, Mousapour Gorji A, Hegedus G, Taller J (2012) PICcalc: an online program to calculate polymorphic information content for molecular genetic studies. Biochem Genet 50:670–672
Nei M (1978) Estimation of average heterozigosity and genetic distance from a small number of individuals. Genetics 89:583–590
Pirmoradi MR (2012) Morphological, physiological, phytochemical and genetical evaluation of Asafoetida in Kerman Province. PhD Thesis, Horticulture Science, Faculty of Agriculture, Tarbiat Modares University, Iran
Rodrigues L, Berg C, Povoa O, Monteiro A (2013) Low genetic diversity and significant structuring in the endangered Mentha cervina populations and its implications for conservation. Biochem Syst Ecol 50:51–61
Rubio-Moraga A, Candel-Perez D, Lucas-Borja ME, Tiscar PA, Vinegla B, Linares JC, Gomez-Gomez L, Ahrazem O (2012) Genetic diversity of Pinus nigra Arn. populations in Southern Spain and Northern Morocco revealed by inter-simple sequence repeat profiles. Int J Mol Sci 13:5645–5658
Sajna N, Kavar T, Sustar-Vozlic J, Kaligaric M (2012) Population genetics of the narrow endemic Hladnikia pastinacifolia RcHB. (Apiaceae) indicates survival in situ during the Pleistocene. Acta Biol Cracov Ser Bot 54(1):84–96
Salami M, Rahimmalek M, Ehtemam MH (2017) Genetic variability of outcross and selfed fennel based on morphological and ISSR markers. J Agric Sci Technol 19:157–172
Shaw RK, Acharya L, Mukherjee AK (2009) Assessment of genetic diversity in a highly valuable medicinal plant Catharanthus roseus using molecular markers. Crop Breed Appl Biotechnol 9:52–59
Singh DR, Srivastava AK, Srivastava A, Srivastava RC (2011) Genetic diversity among three Morinda species using RAPD and ISSR markers. Indian J Biotechnol 10:285–293
Smith WB, Frye CT, Veliz E, Hiebler S, Taylor RC, Hunter KL (2015) Genetic variability of Maryland and West Virginia population of the federally endangered plant Harperella nodosa (Rose) (Apiaceae). Northeast Nat 22(1):106–119
Szenejko M, Smietana P, Stepien E (2016) Genetic diversity of Poa pratensis L. depending on geographical origin and compared with genetic markers. PeerJ. https://doi.org/10.7717/peerj.2489
Talebi M, Rahimmalek M, Norouzi M (2015) Genetic diversity of Thymus daenensis subsp. daenensis using SRAP markers. Biologia 70(4):453–459
Tuncturk M, Ozgokce F (2015) Chemical composition of some Apiaceae plants commonly used in herby cheese in Eastern Antolia. Turk J Agric For 39:55–62
Wang XM, Hou XQ, Zhang YQ, Yang R, Feng SF, Li Y, Ren Y (2012) Genetic diversity of the endemic and medicinally important plant Rheum officinale as revealed by inter-simple sequence repeat (ISSR) markers. Int J Mol Sci 13:3900–3915
Yousefi V, Najaphy A, Zebarjadi A, Safari H (2015) Genetic diversity and geographic dispersion in Thymus spp. as detected by RAPD markers. Philippine J Crop Sci 40(1):82–88
Zietkiewicz E, Rafalske A, Labuda D (1994) Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genome 20:178–183
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Hossein Jafari, S., Sepehry, A., Soltanloo, H. et al. Genetic differentiation between bitter and sweet asafetida plants using ISSR markers. Mol Biol Rep 46, 1069–1078 (2019). https://doi.org/10.1007/s11033-018-4565-1
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DOI: https://doi.org/10.1007/s11033-018-4565-1