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Association of CD209 and CD209L polymorphisms with tuberculosis infection in a Northeastern Brazilian population

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Abstract

Tuberculosis (TB) caused by Mycobacterium tuberculosis, is major cause of morbidity and mortality worldwide. So far, many candidate genes have been investigated for their possible association with TB. Dendritic cell-specific intercellular adhesion molecule 3 (ICAM-3) grabbing non-integrin (DC-SIGN) and Liver/lymph node-specific intercellular adhesion molecule-grabbing non-integrin (L-SIGN), encoded by CD209 and CD209L genes respectively, are known for binding to M. tuberculosis on human dendritic cells and macrophages. We screened 4 single nucleotide polymorphisms (SNPs) in the promoter region of CD209, namely −939G>A (rs735240), −871A>G (rs735239), −336A>G (rs4804803) and −139G>A (rs2287886) and tandem repeat polymorphisms in exon 4 of CD209 and CD209L genes looking for association with TB in a Northeastern Brazilian population (295 subjects, 131 TB patients and 164 healthy controls). The −139G>A and −939G>A SNPs were associated with susceptibility to TB, and in particular with pulmonary and extra-pulmonary forms respectively. The −871A>G and −336A>G SNPs were associated, the first with protection to both pulmonary and extra-pulmonary TB, the latter only with the pulmonary form. An association between GGAG haplotype and protection to TB infection was also found. Also tandem repeat polymorphism in CD209L exon 4 was associated with TB infection. This study provides evidence of an association between CD209 and CD209L polymorphisms and TB development in a Brazilian population, suggesting that variations in these genes may influence the protection and susceptibility to infection caused by M. tuberculosis.

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Acknowledgments

We thank the Laboratory of Immunopathology Keizo Asami, the Department of Genetics, Federal University of Pernambuco, the Graduate Program in Genetics for supporting physical and scientific, as well as FACEPE and CNPq, for financial support. We thank the Program for Technological Development in Tools for Health-PDTIS/FIOCRUZ for use of its facilities.

Conflict of interest

None of the authors has any potential financial conflict of interest related to this manuscript.

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Authors and Affiliations

  1. Department of Genetics, Federal University of Pernambuco (UFPE), Av. Prof. Moraes Rego, 1235 - Cidade Universitária, Recife, PE, Brazil

    Ronaldo Celerino da Silva, Heidi Lacerda Alves da Cruz, Sergio Crovella & Rafael Lima Guimarães

  2. Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco (UFPE), Recife, PE, Brazil

    Ronaldo Celerino da Silva, Heidi Lacerda Alves da Cruz, Sergio Crovella & Rafael Lima Guimarães

  3. Institute for Maternal and Child Health, IRCCS Burlo Garofolo, Trieste, Italy

    Ludovica Segat

  4. Departament of Immunology, Aggeu Magalhães Research Center (CPqAM– FIOCRUZ – Pernambuco), Recife, PE, Brazil

    Haiana Charifker Schindler & Lilian Maria Lapa Montenegro

Authors
  1. Ronaldo Celerino da Silva
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  2. Ludovica Segat
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  3. Heidi Lacerda Alves da Cruz
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  4. Haiana Charifker Schindler
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  5. Lilian Maria Lapa Montenegro
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  7. Rafael Lima Guimarães
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Correspondence to Ronaldo Celerino da Silva.

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da Silva, R.C., Segat, L., da Cruz, H.L.A. et al. Association of CD209 and CD209L polymorphisms with tuberculosis infection in a Northeastern Brazilian population. Mol Biol Rep 41, 5449–5457 (2014). https://doi.org/10.1007/s11033-014-3416-y

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  • DOI: https://doi.org/10.1007/s11033-014-3416-y

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