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Quantitative trait locus analysis of saturated fatty acids in a population of recombinant inbred lines of soybean

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Abstract

Soybean [Glycine max (L.) Merr.] is an important crop which contributes approximately 58% of the world’s oilseed production. Palmitic and stearic acids are the two main saturated fatty acids in soybean oil. Different levels of saturated fatty acids are desired depending on the uses of the soybean oil. Vegetable oil low in saturated fatty acids is preferred for human consumption, while for industrial applications, soybean oil with higher levels of saturated fatty acids is more suitable. The objectives of this study were to identify quantitative trait loci (QTL) for saturated fatty acids, analyze the genetic effects of single QTL and QTL combinations, and discuss the potential of marker-assisted selection in soybean breeding for modified saturated fatty acid profiles. A population of recombinant inbred lines derived from the cross of SD02-4-59 × A02-381100 was grown in five environments and the seed samples from each environment were evaluated for fatty acid content. Genotyping of the population was performed with 516 polymorphic single nucleotide polymorphism markers and 298 polymorphic simple sequence repeat markers. Eight QTL for palmitic acid, five QTL for stearic acid and nine QTL for total saturated fatty acids were detected by composite interval mapping and/or interval mapping, with a high level of consistency or repeatability in multiple environments. Most of these QTL have not been reported previously, with the exception of qPAL-A1 which confirmed the result of a previous study. Significant QTL × QTL interactions were not detected. However, significant QTL × environment interactions were detected in most cases. Comparisons of two-locus and three-locus combinations indicated that cumulative effects of QTL were significant for both palmitic and stearic acids. QTL pyramiding by molecular marker-assisted selection would be an appropriate strategy for improvement of saturated fatty acids in soybean.

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Acknowledgments

This research was supported in part by USB Seed Composition Research project via USDA/ARS, South Dakota Soybean Research & Promotion Council project, USDA-NIFA Hatch project (SD00H374-10), and South Dakota Agricultural Experiment Station. The determination of fatty acids was completed in the USDA/ARS lab in Peoria, IL and the Iowa State University DNA Facility in Ames, IA. The parental line (A02-381100) was provided by Dr. Walter Fehr, Iowa State University.

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  1. David L. Hyten

    Present address: Pioneer Hi-Bred International, 7300 NW 62nd Ave., PO Box 1004, Johnston, IA, 50131, USA

Authors and Affiliations

  1. Plant Science Department, South Dakota State University, Brookings, SD, 57007, USA

    Xianzhi Wang, Guo-Liang Jiang & Marci Green

  2. Crop Production and Protection, GWCC-BLTSVL, USDA/ARS, 5601 Sunnyside Ave, Beltsville, MD, 20705-5139, USA

    Roy A. Scott

  3. Soybean Genomics and Improvement Laboratory, USDA/ARS, 10300 Baltimore Ave, Beltsville, MD, 20705, USA

    David L. Hyten & Perry B. Cregan

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  1. Xianzhi Wang
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Correspondence to Guo-Liang Jiang.

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Wang, X., Jiang, GL., Green, M. et al. Quantitative trait locus analysis of saturated fatty acids in a population of recombinant inbred lines of soybean. Mol Breeding 30, 1163–1179 (2012). https://doi.org/10.1007/s11032-012-9704-0

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  • DOI: https://doi.org/10.1007/s11032-012-9704-0

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