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Biochemical and Biological Characterization of Type-I Collagen from Scomberomorus commerson Skin as a Biomaterial for Medical Applications

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Abstract

Marine collagen, as a biomedical material, has been taken into consideration because of low immunogenicity, biodegradibility, as well as being water soluble, safe, and easily-extractable. We extracted acid-soluble collagen (ASC) from Scomberomorus commerson fish skin found in Persian Gulf, Iran and conducted chemical and physical characterizations using various techniques including thermo gravimetric analysis (TGA), scanning electron microscopy (SEM), X-ray diffraction, Fourier transformation infrared (FTIR), sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE), and MTT assay of the ASC. Our results showed that the yield of ASC was 20.30%. SEM observation demonstrated that the structure of the extracted collagen was fibrils with irregular dense sheet-like structures. SDS-PAGE analysis showed that S.commerson ASC had α (α1and α2) and β chains, presumably belonging to type I collagen. The amount of amino acids and proline in ASC was 18 per 1,000 residues and 90.7 per 706.1 residues, respectively. Also, 27.06% of the collagen was composed of glycine. FTIR analysis and the spectrum X-ray diffraction demonstrated that the S.commerson skin collagen had a triple-helix structure with the ASC denaturation temperatures of 45 °C and 291.89 ºC. Cell activity and proliferation testing demonstrated that S. commerson collagen sample promote cell growth and proliferation and its hydrophilicity leads to cell attachment. In conclusion, the S. commerson fish skin can perform as an affordable collagen source for medical biomaterials and pharmaceutical applications.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This research was funded by Iran University of Medical Sciences (IR.IUMS.REC.1399.1085).

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Authors and Affiliations

  1. Burn Research Center, Iran University of Medical Sciences, Tehran, Iran

    Soheila Naderi Gharahgheshlagh, Tayyeb Ghadimi, Siamak Farokh Forghani & Rana Irilouzadian

  2. Department of Plastic and Reconstructive Surgery, School of Medicine, Hazrat Fatemeh Hospital, Iran University of Medical Sciences, Hazrat-E Fatemeh Hospital, 21Th Alley, Seyed Jamaloddin Asad Abadi St, Tehran, Iran

    Soheila Naderi Gharahgheshlagh, Noorahmad Latifi, Tayyeb Ghadimi, Siamak Farokh Forghani & Negar Taghavian

  3. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

    Naser Amini

  4. Institutes of Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran

    Naser Amini

  5. Department of Natural Resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Ghazaleh Larijani

  6. Department of Tissue Engineering and Regenerative Medicine, Iran University of Medical Sciences, Tehran, Iran

    Saeideh Hatami

  7. Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Sina Bayat Shahbazi

  8. Department of Oral and Maxillofacial Surgery, Dental School, Shahid Beheshti University of Medical Sciences, Daneshjou Blvd, District 1, Tehran, Iran

    Fatemeh Latifi

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  1. Soheila Naderi Gharahgheshlagh
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  2. Noorahmad Latifi
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  3. Tayyeb Ghadimi
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  6. Naser Amini
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  10. Sina Bayat Shahbazi
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  11. Fatemeh Latifi
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Contributions

Conceptualization, Original draft, Conceived and Planned the experiments: SNG; Collagen Extraction and Characterize: SNG, GL, SBS; Data Collection, Management and Reporting, NAL, FL; Data Analysis and Interpretation: NA, TG; Draft Preparation, Manuscript Writing, Editing: SNG, NT, RI, SH.

Corresponding authors

Correspondence to Noorahmad Latifi or Fatemeh Latifi.

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Conflict of interest

The authors declare no conflicts of interest.

Ethical Approval

The study was approved by the ethics Committee of the Iran University of medical sciences (No. IR.IUMS.REC.1399.1085).

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Not applicable. The skin of S. commerson was collected and purchased from a local market in Tehran, Iran which they had sold the meat of them and the skins were their waste.

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Naderi Gharahgheshlagh, S., Latifi, N., Ghadimi, T. et al. Biochemical and Biological Characterization of Type-I Collagen from Scomberomorus commerson Skin as a Biomaterial for Medical Applications. Int J Pept Res Ther 29, 56 (2023). https://doi.org/10.1007/s10989-023-10534-1

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