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Examination of the peripheral nerve and muscle damage in cyclophosphamide monotherapy with DSC in animal models

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Abstract

Polyneuropathy is defined as a simultaneous malfunction of several peripheral nerves, which could be a side effect of cancer therapy; however, this is reported to occur rarely and difficult to prove. The purpose of this study was to introduce at the first time the calorimetry in the diagnosis of neuropathy in an experimental animal model. Adult guinea pigs (n = 55, in 11 different groups) were injected intraperitoneally with the dose of cyclophosphamide that is comparable to the human dosage. Animals were euthanized; nerve (ischiadicus) and muscle (gastrocnemius) samples were analyzed by a SETARAM Micro-DSC. The denaturation temperatures as well as the calorimetric enthalpies were determined. The thermal parameters of denaturation of the samples varied depending on the therapeutic cyclophosphamide doses. The nerves were more sensitive to chemotherapy, compared to the muscles. An interesting body side effect of treatment was observed. The DSC is an appropriate tool to follow the effects of cyclophosphamide on peripheral nerves and muscles, which were found dose dependent.

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Acknowledgements

The authors express their thanks to Angéla Dömse for the technical help in the drug treatment of the animals. This work was supported by Grants OTKA CO-272 (for Dénes Lőrinczy). The present scientific contribution is dedicated to the 650th anniversary of the foundation of the University of Pécs, Hungary.

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Authors and Affiliations

  1. Clinics of Radiology, University of Pécs, H-7624 Pécs Szigeti str. 12, Pécs, Hungary

    Péter Farkas

  2. Institute of Forensic Medicine, University of Pécs, H-7624 Pécs Szigeti str. 12, Pécs, Hungary

    Franciska Könczöl

  3. Institute of Biophysics School of Medicine, University of Pécs, H-7624 Pécs Szigeti str. 12, Pécs, Hungary

    Dénes Lőrinczy

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  1. Péter Farkas
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  2. Franciska Könczöl
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  3. Dénes Lőrinczy
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Correspondence to Dénes Lőrinczy.

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Farkas, P., Könczöl, F. & Lőrinczy, D. Examination of the peripheral nerve and muscle damage in cyclophosphamide monotherapy with DSC in animal models. J Therm Anal Calorim 126, 47–53 (2016). https://doi.org/10.1007/s10973-016-5253-9

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  • DOI: https://doi.org/10.1007/s10973-016-5253-9

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