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Influence of oxidative injury and monitoring of blood plasma by DSC on breast cancer patients

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Abstract

Damage caused by oxidative stress is involved in many types of diseases, including breast cancer. Our aim was to detect the oxidative stress parameters and blood plasma changes with differential scanning calorimetry (DSC) in breast cancer patients. The study included 40 adult breast cancer women who were grouped according to tumor diameter, regional lymph node metastases, proliferative activity, receptor status and postoperative chemotherapy. To monitor oxidative stress, malondialdehyde, oxygen free radicals (OFRs), activity of myeloperoxidase (MPO), superoxide dismutase (SOD) and catalase (CAT) were measured. Denaturation of plasma components was detected in Setaram Micro DSC-II calorimeter. The total production of OFRs, the MPO activity and lipidperoxidation were significantly increased in each breast cancer patients considering the tumor size, the metastatic lymph nodes, the proliferation activity and receptor status compared with healthy controls (p < 0.05). These pro-oxidants were slightly elevated without chemotherapy, but their values were increased significantly in chemotherapy-receiving group. The activity of SOD and CAT was significantly decreased in all groups, and in regard to the chemotherapy, they were changed significantly parallel to the severity of disease. Regarding to both the increased tumor diameter and the increased number of affected lymph nodes, DSC measurements showed a strong relationship between the maximum excess heat capacity (C pmax) of the blood plasma and the severity of disease. The study demonstrated that oxidative stress is implicated in breast carcinoma and chemotherapy aggravates these changes which confirmed the plasma DSC measurements also.

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Acknowledgements

This work was supported by Grants OTKA CO-272 (for Dénes Lőrinczy). The present scientific contribution is dedicated to the 650th anniversary of the foundation of the University of Pécs, Hungary.

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Authors and Affiliations

  1. Surgery Clinic, Medical School, University of Pécs, Rákóczi Str. 2, Pecs, 7624, Hungary

    István Zapf

  2. Department of Dermatology, Venereology and Oncodermatology, Medical School, University of Pécs, Akác Str. 1, Pecs, 7632, Hungary

    Medhi Moezzi

  3. Government Office of Baranya County, József A. Str. 10, Pecs, 7623, Hungary

    Tamás Fekecs

  4. General and Vascular Surgical Department, Zala County Hospital, Zrínyi Str. 1, Zalaegerszeg, 8900, Hungary

    Klára Nedvig

  5. Department of Biophysics, Medical School, University of Pécs, Szigeti Str. 12, Pecs, 7624, Hungary

    Dénes Lőrinczy

  6. Department of Surgical Research and Techniques, Medical Faculty, Semmelweis University, Nagyvárad Str. 4, Budapest, 1089, Hungary

    Andrea Ferencz

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  1. István Zapf
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  2. Medhi Moezzi
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  3. Tamás Fekecs
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  4. Klára Nedvig
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  5. Dénes Lőrinczy
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  6. Andrea Ferencz
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Correspondence to Andrea Ferencz.

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Zapf, I., Moezzi, M., Fekecs, T. et al. Influence of oxidative injury and monitoring of blood plasma by DSC on breast cancer patients. J Therm Anal Calorim 123, 2029–2035 (2016). https://doi.org/10.1007/s10973-015-4642-9

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  • DOI: https://doi.org/10.1007/s10973-015-4642-9

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