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Radiolytically Depolymerized Low Molecular-Weight Chitosan (ICH) and Sodium Alginate (ISA) Improve Growth Attributes, Physiological Performance and the Production of Steviol Glycosides (SGs) of S. rebaudiana (Bertoni)

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Abstract

The leaves of Stevia rebaudiana (Bertoni) are the source of several medicinally important bioactive compounds, including steviol glycosides (SGs), primarily accountable for the sweetness of the plant, and phenolic compounds, which offer a wide variety of therapeutic potential against various diseases. Two separate pot experiments were conducted simultaneously in order to investigate the influence of foliar-spray treatments at different concentrations (40, 80, 120, and 160 mg L−1) of irradiated chitosan (ICH) and irradiated sodium alginate (ISA) on the performance of S. rebaudiana with regard to its growth, physiological activities, and production of steviol glycosides measured at 90 days after planting. The growth and physiological attributes of Stevia were significantly improved as a result of foliar sprays of ICH and ISA. Of the treatments, 80 mg L−1 of ICH and 120 mg L−1 of ISA performed the best and resulted in the highest values of all studied parameters compared to the water-spray control. On area-percentage basis, the respective treatments of ICH and ISA also led to an increase in the leaf contents of steviol glycosides, viz., Stevioside by 57.14% and 51.13% and (Rebaudioside A) by 43.28% and 36.64% respectively, as well as other bioactive compounds of S. rebaudiana leaf such as D-Allose sugar (by 33.17% and 29.98%), Neophytadiene (by 57.40% and 53.21%), and β-amyrin (by 16.31% and 13.81%), respectively, compared to the control treatment. Findings of the present study demonstrated that foliar-sprays of γ-irradiated chitosan and sodium alginate improved growth as well as physiological performance of S. rebaudiana, triggering the production of important steviol glycosides and other bioactive constituents of Stevia through up-regulating the secondary metabolism.

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Acknowledgements

The first author is highly thankful to University Grants Commission (UGC), New Delhi (India), for providing financial assistance to the first author. We also grateful to the Bhabha Atomic Research Centre (BARC), Mumbai, Maharashtra (India) for their assistance in providing and gamma-irradiation of the chitosan and sodium alginate for the present study.

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Authors and Affiliations

  1. Department of Botany, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India

    Sangram Singh, Sarika Singh, Urooj Hassan Bhat, Aman Sobia Chishti, Akshay Chauhan & M. Masroor A. Khan

  2. Botany Section, Women’s College, Aligarh Muslim University, Aligarh, India

    Moin Uddin

  3. Faculty of Agricultural Science, Aligarh Muslim University, Aligarh, India

    Khan Bilal Mukhtar Ahmed

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  1. Sangram Singh
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Contributions

SS: Methodology, Investigation, Formal analysis, Visualization, Writing – original draft. MU: Supervision, Methodology, Formal analysis, Writing – review & editing. SS: Formal analysis, Visualization. KBMA: Conceptualization, Resources, Methodology, Formal analysis. UHB: Formal analysis, Visualization. ASC: Formal analysis, Visualization. MMAK: Supervision, Conceptualization, Resources, Methodology, Formal analysis. AC: Formal analysis, Visualization. All authors have read and agreed to the published present version of the manuscript.

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Correspondence to Sangram Singh.

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Singh, S., Uddin, M., Singh, S. et al. Radiolytically Depolymerized Low Molecular-Weight Chitosan (ICH) and Sodium Alginate (ISA) Improve Growth Attributes, Physiological Performance and the Production of Steviol Glycosides (SGs) of S. rebaudiana (Bertoni). J Polym Environ (2024). https://doi.org/10.1007/s10924-023-03149-6

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