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Scleroglucan Production by Sclerotium rolfsii ATCC 201126 from Amylaceous and Sugarcane Molasses-Based Media: Promising Insights for Sustainable and Ecofriendly Scaling-Up

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Abstract

Scleroglucan is a β-glucan exopolysaccharide (EPS) efficiently produced by Sclerotium rolfsii ATCC 201126, with attractive properties for a wide range of industries. Its production was herein comparatively assessed with nine alternative C- and twelve N-sources. When comparing conventional sucrose-based Production Medium PM20 (8.41 g C/L + NaNO3 as N-source) at shake-flask-scale vs. alternative C-source versions, soluble starch and sugarcane molasses led to efficient EPS production. At bioreactor scale, starch-based medium led to highest EPS production (7.95 g/L), recovery (~ 52%) and productivities (0.11 g EPS/L h; 0.018 g EPS/gbiomass h). Molasses, though leading to lower EPS production (5.11 g/L), could be envisaged as a promising agroindustrial sub-product for adding value and innovation. Oxalate side-product varied with C- and N-sources, with no clear detrimental relationship with EPS production. Agroindustrial sub-products showed then to be suitable as alternative substrates for efficient, low-cost, and scalable EPS production, thus opening new perspectives for medium reformulation and sustainable EPS production.

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Fig. 1

source: 1 corn starch, 2 soluble starch, 3 cassava starch. Activities were confronted against different substrates (soluble, corn and cassava starch at 40 g/L, starch-Azure at 0.5 g/L) and with different detection methods (direct or developed with I2). Results correspond to 24 h of incubation at 37 °C. Positive controls include: α-amylase from A. oryzae Type X-A (crude) (α-A), β-amylase from sweet potato Type I-B (β-A), and amyloglucosidase from A. niger (AG). For further details, see “Material and Methods” section

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Acknowledgements

The study was financially supported by the National Council for Scientific and Technical Research (CONICET) (Grant PIP0976), the National University of Catamarca (UNCa) (Grant PIO-UNCa 0100054), and the National Agency for the Promotion of Science and Technology (ANPCyT) along with the Deutsche Forschungsgemeinschaft (DFG) (Bilateral Cooperation project CONICET-MINCyT-DFG level II). Authors gratefully acknowledge Lic. María Florencia Ladetto for critical analyses of FT-IR spectra.

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  1. Jaime Daniel Babot

    Present address: Centro de Referencia para Lactobacilos, CERELA-CONICET, T4000 ILC, SM Tucumán, Tucumán, Argentina

  2. Jochen Schmid

    Present address: Department of Biotechnology and Food Science, Norwegian University of Science and Technology, 7034, Trondheim, Trøndelag, Norway

Authors and Affiliations

  1. Mycodiversity & Mycoprospection Lab, Planta Piloto de Procesos Industriales Microbiológicos, PROIMI-CONICET, Av. Belgrano y Pje. Caseros, T4001 MVB, SM Tucumán, Tucumán, Argentina

    Alejandra Leonor Valdez, Jaime Daniel Babot, Osvaldo Daniel Delgado & Julia Inés Fariña

  2. Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, 94315, Straubing, Germany

    Jochen Schmid

  3. Faculty of Exact and Natural Sciences, National University of Catamarca, K4700, Catamarca, Argentina

    Osvaldo Daniel Delgado

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Valdez, A.L., Babot, J.D., Schmid, J. et al. Scleroglucan Production by Sclerotium rolfsii ATCC 201126 from Amylaceous and Sugarcane Molasses-Based Media: Promising Insights for Sustainable and Ecofriendly Scaling-Up. J Polym Environ 27, 2804–2818 (2019). https://doi.org/10.1007/s10924-019-01546-4

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