Abstract
Displacement of enzyme mimic nanomaterials has been developed due to disadvantages of natural enzymes. Peroxidase reaction could be used for H2O2 colorimetric evaluation in different media. We aim to develop a new method based on the best catalytic activity of metal Nanozyms. A method for H2O2 detection was carried out by TMB peroxidation of metal nanozymes. In this way peroxidase activity of Ag-nano zeolite Y, Cu-nano zeolite Y, Zn-nano zeolite Y and Ag, Cu, Zn-nano zeolite Y was evaluated by Central Composite Design. Total of 28.42% from three metals was exchanged in multi metal nano zeolite Y (mMy) with higher peroxidase- like activity. Nanozyme Km and Vm were evaluated 0.076 and 8.76 × 108, respectively which produced fast and sensitive catalytic reaction. H2O2 method detection carried out by LOD level of 0.12 ppm, and recovery parameters of 99%, in the linear range of 0.5–50 ppm. The mMy nanozyme was validated for H2O2 colorimetric detection. In conclusion an applicable way as a fast, sensitive, accurate and reproducible method has been suggested for occupational exposure monitoring. Performance of validated method is higher than international OSHAVI-6 method.
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Notes
Horseradish peroxidase.
References
B. Peng, J. Xu, M. Fan, Y. Guo, Y. Ma, M. Zhou et al., Smartphone colorimetric determination of hydrogen peroxide in real samples based on B, N, and S co-doped carbon dots probe. Anal. Bioanal. Chem. 412(4), 861–870 (2020)
X. Zhu, Y. Xue, S. Han, W. Chen, M. Fu, Y. Gao et al., V2O5-montmorillonite nanocomposites of peroxidase-like activity and their application in the detection of H2O2 and glutathione. Appl. Clay Sci. 195, 105718 (2020)
F. Qiao, L. Chen, X. Li, L. Li, S.J.S. Ai, A.B. Chemical, Peroxidase-like activity of manganese selenide nanoparticles and its analytical application for visual detection of hydrogen peroxide and glucose. Sens. Actuators, B Chem. 193, 255–262 (2014)
B. Maojuan, X. Chengcheng, H. Xuanye, L. Yanan, Jun WJJoN, Nanotechnology. Peroxidase mimic activities of copper selenide (CuSe) nanoplates for sensing H2O2 and L-cysteine. J. Nanosci. Nanotechnol. 20(9), 5369–5375 (2020)
P. Saha, A. Maharajan, P.K. Dikshit, B.S. Kim, Rapid and reusable detection of hydrogen peroxide using polyurethane scaffold incorporated with cerium oxide nanoparticles. Korean J. Chem. Eng. 36(12), 2143–2152 (2019)
H. Liu, L. Zhu, H. Ma, J. Wen, H. Xu, Y. Qiu et al., Copper (II)-coated Fe3O4 nanoparticles as an efficient enzyme mimic for colorimetric detection of hydrogen peroxide. Microchim. Acta 186(8), 1–9 (2019)
Y. Zhang, F. Wang, C. Liu, Z. Wang, L. Kang, Y. Huang et al., Nanozyme decorated metal–organic frameworks for enhanced photodynamic therapy. ACS Nano 12(1), 651–661 (2018)
X. Lin, Y. Liu, Z. Tao, J. Gao, J. Deng, J. Yin et al., Nanozyme-based bio-barcode assay for high sensitive and logic-controlled specific detection of multiple DNAs. Biosens. Bioelectron. 94, 471–477 (2017)
J. Yao, Y. Cheng, M. Zhou, S. Zhao, S. Lin, X. Wang et al., ROS scavenging Mn3O4 nanozymes for in vivo anti-inflammation. Chem. Sci 9(11), 2927–2933 (2018)
T. Zhang, F. Tian, L. Long, J. Liu, X. Wu, Diagnosis of rubella virus using antigen-conjugated Au@ Pt nanorods as nanozyme probe. Int. J. Nanomed. (2018). https://doi.org/10.2147/IJN.S171429
F. Ratto, P. Matteini, F. Rossi, R. Pini, Size and shape control in the overgrowth of gold nanorods. J. Nanopart. Res. 12, 2029–2036 (2010)
Y. Li, X. Jian, S. Zhou, Y. Lu, C. Zhao, Z. Gao et al., Protein shell-encapsulated Pt clusters as continuous O2-supplied biocoats for photodynamic therapy in hypoxic cancer cells. ACS Appl. Mater. Interfaces 11(19), 17215–17225 (2019)
J. Ali, N. Ali, L. Wang, H. Waseem, G. Pan, Revisiting the mechanistic pathways for bacterial mediated synthesis of noble metal nanoparticles. J. Microbiol. Methods 159, 18–25 (2019)
T.K. Sau, A.L. Rogach, Nonspherical noble metal nanoparticles: colloid-chemical synthesis and morphology control. Adv. Mater. 22(16), 1781–1804 (2010)
W. He, X. Han, H. Jia, J. Cai, Y. Zhou, Z. Zheng, AuPt alloy nanostructures with tunable composition and enzyme-like activities for colorimetric detection of bisulfide. Sci. Rep. 7(1), 40103 (2017)
Q. Wang, L. Zhang, C. Shang, Z. Zhang, S.J.C.C. Dong, Triple-enzyme mimetic activity of nickel–palladium hollow nanoparticles and their application in colorimetric biosensing of glucose. Chem. Commun. 52(31), 5410–5413 (2016)
V. Mariyappan, M. Keerthi, S.-M. Chen, G. Boopathy, Facile synthesis of α-Sm2S3/MoS2 bimetallic sulfide as a high-performance electrochemical sensor for the detection of antineoplastic drug 5-fluorouracil in a biological samples. J. Electrochem. Soc. 167(11), 117506 (2020)
I. Taurino, G. Sanzò, R. Antiochia, C. Tortolini, F. Mazzei, G. Favero et al., Recent advances in third generation biosensors based on Au and Pt nanostructured electrodes. TrAC, Trends Anal. Chem. 79, 151–159 (2016)
W. Qin, L. Su, C. Yang, Y. Ma, H. Zhang, X. Chen, Chemistry f Colorimetric detection of sulfite in foods by a TMB–O2–CO3O4 nanoparticles detection system. J. Agric. Food Chem 62(25), 5827–5834 (2014)
J. Mu, X. Zhao, J. Li, E.-C. Yang, X.-J. Zhao, Novel hierarchical NiO nanoflowers exhibiting intrinsic superoxide dismutase-like activity. J. Mater. Chem. B 4(31), 5217–5221 (2016)
Q. Han, X. Wang, X. Liu, Y. Zhang, S. Cai, C. Qi et al., MoO3− x nanodots with dual enzyme mimic activities as multifunctional modulators for amyloid assembly and neurotoxicity. J. Colloid and Interface Sci. 539, 575–584 (2019)
X. Ai, L. Wu, M. Zhang, X. Hou, L. Yang, C. Zheng, Chemistry f. Analytical method for the determination of trace toxic elements in milk based on combining Fe3O4 nanoparticles accelerated UV fenton-like digestion and solid phase extraction. J. Agric. Food Chem. 62(34), 8586–8593 (2014)
F.F. Peng, Y. Zhang, N. Gu, Size-dependent peroxidase-like catalytic activity of Fe3O4 nanoparticles. Chinese Chem. Lett. 19(6), 730–733 (2008)
K. Zhang, W. Zuo, Z. Wang, J. Liu, T. Li, B. Wang, Z. Yang, A simple route to CoFe2O4 nanoparticles with shape and size control and their tunable peroxidase-like activity. RCS Adv. (2015). https://doi.org/10.1039/C4RA15675G
H.-P. Feng, L. Tang, G.-m Zeng, Y. Zhou, Y.-C. Deng, X. Ren et al., Core-shell nanomaterials: applications in energy storage and conversion. Adv. Colloid Interface Sci. 267, 26–46 (2019)
A. Ángeles-Pascual, J. Piñón-Hernández, M. Estevez-González, U. Pal, S. Velumani, R. Pérez, R.J.M.C. Esparza, Structure, magnetic and cytotoxic behaviour of solvothermally grown Fe3O4@ Au core-shell nanoparticles. Mater. Charact. 142, 237–244 (2018)
M. Ye, Q. Zhang, Y. Hu, J. Ge, Z. Lu, L. He et al., Magnetically recoverable core–shell nanocomposites with enhanced photocatalytic activity. Chem. A Eur. J. 16(21), 6243–6250 (2010)
J. Lu, B. Fu, M.C. Kung, G. Xiao, J.W. Elam, H.H. Kung, P.C.J.S. Stair, Coking-and sintering-resistant palladium catalysts achieved through atomic layer deposition. Eur. MPC 335(6073), 1205–1208 (2012)
Q. Liu, A. Zhang, R. Wang, Q. Zhang, D.J.N. Cui, A review on metal-and metal oxide-based nanozymes: properties, mechanisms, and applications. Nano-Micro Lett. 13, 1–53 (2021)
S. Rauf, N. Ali, Z. Tayyab, M.Y. Shah, C.P. Yang, J. Hu et al., Ionic liquid coated zerovalent manganese nanoparticles with stabilized and enhanced peroxidase-like catalytic activity for colorimetric detection of hydrogen peroxide. Mater. Res. Exp. 7(3), 035018 (2020)
L. Alvarado-Ramírez, M. Rostro-Alanis, J. Rodríguez-Rodríguez, J.E. Sosa-Hernández, E.M. Melchor-Martínez, H.M. Iqbal, R.J.B. Parra-Saldívar, Enzyme (single and multiple) and nanozyme biosensors: recent developments and their novel applications in the water-food-health nexus. Biosensors 11(11), 410 (2021)
M. Liang, X. Yan, Nanozymes: from new concepts, mechanisms, and standards to applications. Am. Chem. Soc. 52(8), 2190–2200 (2019)
A.H. Sharifnezhad, K. Dashtian, F. Amourizi, R. Zare-Dorabei, Development of peptide impregnated V/Fe bimetal Prussian blue analogue as Robust nanozyme for colorimetric fish freshness assessment. Anal. Chimica Acta. 1237, 340555 (2023)
Z. Wang, P. Ju, Y. Zhang, F. Jiang, H. Ding, C.J.M.A. Sun, CoMoO4 nanobelts as efficient peroxidase mimics for the colorimetric determination of H2O2. Microchim. Acta 187(8), 1–14 (2020)
G. Singh, A. Kushwaha, M. Sharma, Compounds Intriguing peroxidase-mimic for H2O2 and glucose sensing: a synergistic Ce2 (MoO4) 3/rGO nanocomposites. J. Alloys Compd. 825, 154134 (2020)
V.P. Pandey, M. Awasthi, S. Singh, S. Tiwari, U.N. Dwivedi, A comprehensive review on function and application of plant peroxidases. Biochem. Anal. Biochem. 6(1), 308 (2017)
H.Y. Shin, T.J. Park, M. Kim, Recent research trends and future prospects in nanozymes. J. Nanomaterials. (2015). https://doi.org/10.1155/2015/756278
J.E. Giaretta, H. Duan, F. Oveissi, S. Farajikhah, F. Dehghani, S. Naficy, Interfaces flexible sensors for hydrogen peroxide detection: a critical review. ACS Appl. Mater. Interfaces 14(18), 20491–20505 (2022)
A. Pratsinis, G.A. Kelesidis, S. Zuercher, F. Krumeich, S. Bolisetty, R. Mezzenga et al., Enzyme-mimetic antioxidant luminescent nanoparticles for highly sensitive hydrogen peroxide biosensing. ACS Nano 11(12), 12210–12218 (2017)
J. Wu, Y. Wu, L. Lu, D. Zhang, X. Wang, Single-atom Au catalyst loaded on CeO2: a novel single-atom nanozyme electrochemical H2O2 sensor. Talanta Open 4, 100075 (2021)
W. Zhang, W. Liu, P. Li, F. Huang, H. Wang, B. Tang, Rapid-response fluorescent probe for hydrogen peroxide in living cells based on increased polarity of C-B bonds. Anal. Chem. 87(19), 9825–9828 (2015)
A.S. Ivanova, A.D. Merkuleva, S.V. Andreev, K. Sakharov, Method for determination of hydrogen peroxide in adulterated milk using high performance liquid chromatography. Food Chem. 283, 431–436 (2019)
A. Swaidan, A. Addad, J.-F. Tahon, A. Barras, J. Toufaily, T. Hamieh et al., Ultrasmall CuS-BSA-Cu3 (PO4) 2 nanozyme for highly efficient colorimetric sensing of H2O2 and glucose in contact lens care solutions and human serum. Anal. Chim. Acta 1109, 78–89 (2020)
Z. Ahmadzadeh, M. Ranjbar, Plasmonic MoO3-x nanosheets by anodic oxidation of molybdenum for colorimetric sensing of hydrogen peroxide. Anal. Chim. Acta 1198, 339529 (2022)
F. Zarif, S. Rauf, S. Khurshid, N. Muhammad, A. Hayat, A. Rahim et al., Effect of pyridinium based ionic liquid on the sensing property of Ni0 nanoparticle for the colorimetric detection of hydrogen peroxide. J. Molecular Struct. 1219, 128620 (2020)
V.M. Aroutiounian, Hydrogen peroxide semiconductor sensors. J. Contemp. Phys. 56(4), 332–351 (2021)
F. Zarif, S. Khurshid, N. Muhammad, M. Zahid Qureshi, N.S.J.C. Shah, Colorimetric sensing of hydrogen peroxide using ionic-liquid-sensitized zero-valent copper nanoparticle (nZVCu). Chem. Eur. 5(20), 6066–6074 (2020)
N. Mucci, S. Dugheri, A. Bonari, A. Farioli, V. Rapisarda, G. Garzaro et al., Health risk assessment related to hydrogen peroxide presence in the workplace atmosphere–Analytical methods evaluation for an innovative monitoring protocol. Int. J. Occup. Med. Environ. Health (2020). https://doi.org/10.13075/ijomeh.1896.01508
V.M.J.S. Aroutiounian, Transducers properties of hydrogen peroxide sensors made from nanocrystalline materials. Sens. Transducers J. 223(7), 9–21 (2018)
Z. Moradpour, M. Helmi Kohnehshahri, M. Vahabi Shekarloo, V. Jalili, R.J.C. Zendehdel, P. Science, Peroxidase-like reaction by a synergistic inorganic catalyst colloid: a new method for hydrogen peroxide detecting in air samples. Colloid Polym. Sci. 299(10), 1567–1575 (2021)
Q. Pan, Y. Kong, K. Chen, M. Mao, X. Wan, X. She et al., A colorimetric assay for the detection of glucose and H2O2 based on Cu-Ag/g-C3N4/ZIF hybrids with superior peroxidase mimetic activity. Molecules 25(19), 4432 (2020)
T. Cheng, X. Li, P. Huang, H. Wang, M. Wang, W.J.M.A. Yang, Colorimetric and electrochemical (dual) thrombin assay based on the use of a platinum nanoparticle modified metal-organic framework (type Fe-MIL-88) acting as a peroxidase mimic. Microchim. Acta 186(2), 1–8 (2019)
N.H. Abdul Halim, Y.H. Lee, R.S.P.M. Marugan, U.J.B. Hashim, Mediatorless impedance studies with titanium dioxide conjugated gold nanoparticles for hydrogen peroxide detection. Biosensors 7(3), 38 (2017)
A. Ahmed, P. John, M.H. Nawaz, A. Hayat, M. Nasir, Zinc-doped mesoporous graphitic carbon nitride for colorimetric detection of hydrogen peroxide. ACS Appl. Nano Mater.x 2(8), 5156–5168 (2019)
O. Adeniyi, S. Sicwetsha, P. Mashazi, Interfaces Nanomagnet-silica nanoparticles decorated with Au@ Pd for enhanced peroxidase-like activity and colorimetric glucose sensing. ACS Appl. Mater. Interfaces 12(2), 1973–1987 (2019)
Y. Chai, W. Dai, G. Wu, N. Guan, L. Li, Confinement in a zeolite and zeolite catalysis. Acc. Chem. Res 54(13), 2894–2904 (2021)
X. Cheng, L. Huang, X. Yang, A.A. Elzatahry, A. Alghamdi, Y. Deng, Science i. Rational design of a stable peroxidase mimic for colorimetric detection of H2O2 and glucose: a synergistic CeO2/Zeolite Y nanocomposite. J. Colloid and Interface Sci. 535, 425–435 (2019)
H. Derakhshankhah, S. Jafari, S. Sarvari, E. Barzegari, F. Moakedi, M. Ghorbani et al., Biomedical applications of zeolitic nanoparticles, with an emphasis on medical interventions. Int. J. Nanomed. 15, 363 (2020)
S. Demirci, Z. Ustaoğlu, G.A. Yılmazer, F. Sahin, N. Baç, Biotechnology. Antimicrobial properties of zeolite-X and zeolite-A ion-exchanged with silver, copper, and zinc against a broad range of microorganisms. Appl. Biochem. Biotechnol. 172, 1652–1662 (2014)
F. Morante-Carballo, N. Montalván-Burbano, P. Carrión-Mero, N.J.S. Espinoza-Santos, Cation exchange of natural zeolites: worldwide research. Sustainability 13(14), 7751 (2021)
L. Guczi, D. Bazin, Structure and selectivity of metal catalysts: revisiting bimetallic zeolite systems. Appl. Catal. A: General 188(1–2), 163–174 (1999)
H. Ramezani, S.N. Azizi, S.R.J.S. Hosseini, A.B. Chemical, NaY zeolite as a platform for preparation of Ag nanoparticles arrays in order to construction of H2O2 sensor. Sens. Actuators, B Chem. 248, 571–579 (2017)
W. Yang, J. Li, J. Yang, Y. Liu, Z. Xu, X. Sun et al., Biomass-derived hierarchically porous CoFe-LDH/CeO2 hybrid with peroxidase-like activity for colorimetric sensing of H2O2 and glucose. J. Alloys Compd. 815, 152276 (2020)
L. Gao, J. Zhuang, L. Nie, J. Zhang, Y. Zhang, N. Gu et al., Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat. Nanotechnol. 2(9), 577–583 (2007)
Y.-l Dong, H.-G. Zhang, Z.U. Rahman, L. Su, X.-J. Chen, J. Hu, X.-G. Chen, Graphene oxide–Fe3O4 magnetic nanocomposites with peroxidase-like activity for colorimetric detection of glucose. Nanoscale 4(13), 3969–3976 (2012)
L. Sun, Y. Ding, Y. Jiang, Q.J.S. Liu, A.B. Chemical, Montmorillonite-loaded ceria nanocomposites with superior peroxidase-like activity for rapid colorimetric detection of H2O2. Sens. Actuators, B Chem. 239, 848–856 (2017)
X. Zhu, P. Song, S. Hou, H. Zhao, Y. Gao, T. Wu, Q. Liu, Synthesis of Ag nanoparticles supported on magnetic halloysite nanozyme for detection of H2O2 in milk and serum. Appl. Clay Sci. 242, 107022 (2023)
Y. Wang, X. Liu, M. Wang, X. Wang, W. Ma, J.J.S. Li, A.B. Chemical, Facile synthesis of CDs@ ZIF-8 nanocomposites as excellent peroxidase mimics for colorimetric detection of H2O2 and glutathione. Sens. Actuators B: Chem. 329, 129115 (2021)
Y. Mirzaei, A. Gholami, A. Sheini, M.M. Bordbar, An origami-based colorimetric sensor for detection of hydrogen peroxide and glucose using sericin capped silver nanoparticles. Sci. Rep. 13(1), 7064 (2023)
F. Xia, Q. Shi, Z.J.D.T. Nan, Facile synthesis of Cu-CuFe2O4 nanozymes for sensitive assay of H2O2 and GSH. Dalton Trans. 49(36), 12780–12792 (2020)
Y. Ding, H. Liu, L.-N. Gao, M. Fu, X. Luo, X. Zhang et al., Fe-doped Ag2S with excellent peroxidase-like activity for colorimetric determination of H2O2. J. Alloy. Compd. 785, 1189–1197 (2019)
L. Zhang, M. Chen, Y. Jiang, M. Chen, Y. Ding, Q.J.S. Liu, A.B. Chemical, A facile preparation of montmorillonite-supported copper sulfide nanocomposites and their application in the detection of H2O2. Sens. Actuators, B Chem. 239, 28–35 (2017)
T. Zhan, J. Kang, X. Li, L. Pan, G. Li, W.J.S. Hou, A.B. Chemical, NiFe layered double hydroxide nanosheets as an efficiently mimic enzyme for colorimetric determination of glucose and H2O2. Sens. Actuators, B Chem. 255, 2635–2642 (2018)
C. Cui, Q. Wang, Q. Liu, X. Deng, T. Liu, D. Li et al., Porphyrin-based porous organic framework: an efficient and stable peroxidase-mimicking nanozyme for detection of H2O2 and evaluation of antioxidant. Sens. Actuators B Chem. 277, 86–94 (2018)
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Ravannakhjavani, F., Dehghan, S.F., Panahi, D. et al. Multi-metal Nanozyme Properties for Colorimetric Peroxidase Reaction: Overview of an Applicable Method Validation for H2O2 Detection. J Inorg Organomet Polym 34, 818–826 (2024). https://doi.org/10.1007/s10904-023-02847-x
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DOI: https://doi.org/10.1007/s10904-023-02847-x