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Fluorescence Spectroscopy Based Identification of Pseudomonas Aeruginosa and Escherichia Coli Suspensions

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A Correction to this article was published on 21 May 2024

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Abstract

In this article, Fluorescence spectroscopy has been employed for the identification of Pseudomonas aeruginosa (PA) and Escherichia coli (E. coli) in water suspension. Emission spectra of PA and E. coli suspensions have been acquired by using excitation wavelengths from 270 to 420 nm with steps of 10 nm to explore their spectral features. It has been found that the emission spectra of tryptophan, tyrosine, NADH and FAD, being the intracellular biomolecules present in both bacteria, can be used as fingerprints for their identification, differentiation and quantification. Both bacterial strains can clearly be differentiated from water and from each other by using λex 270–290 nm through spectral analysis and from λex: 300–500 nm by applying statistical analysis. Furthermore, calibration curves for different bacterial loads of PA and E. coli suspensions have been produced between colonies forming units per ml (CFUs/ml) the integrated intensities of their emission spectra. CFUs/ml of both bacterial suspensions have been determined through plate count method which was used as cross-reference for the analysis of emission spectra of both bacterial suspensions. These curves may be used to estimate CFU/ml of both PA and E. coli in unknown water suspensions by determining the integrating intensity of their emission spectra.

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No datasets were generated or analysed during the current study.

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Acknowledgements

We highly acknowledge Higher Education Commission of Pakistan for the award of NRPU project No: 20-14617/NRPU/R&D/HEC/2021 2021.

Funding

This work is funded through a NRPU research project 20-14617/NRPU/R&D/HEC/2021 2021 awarded by Higher Education Commission of Pakistan.

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Authors and Affiliations

  1. National Institute of Lasers and Optronics College, Pakistan Institute of Engineering and Applied Sciences, 45650, Nilore, Islamabad, Pakistan

    Sana Imtiaz & Muhammad Saleem

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  1. Sana Imtiaz
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  2. Muhammad Saleem
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Contributions

Sana Imtiaz did experimental measurements and analysis of the measured data, Muhammad Saleem conceived the concept, did analysis, wrote and reviewed the manuscript. Both authors have read and approved the final manuscript.

Corresponding author

Correspondence to Muhammad Saleem.

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The authors declare no competing interests.

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Ethical approval was not needed because no human or animal was involved for conducting present study.

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The original online version of this article was revised: The caption of Figure 3 is correct but the figure legend/labeling on the figure is incorrect.

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Imtiaz, S., Saleem, M. Fluorescence Spectroscopy Based Identification of Pseudomonas Aeruginosa and Escherichia Coli Suspensions. J Fluoresc (2024). https://doi.org/10.1007/s10895-024-03608-w

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