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Rapid Biological Synthesis of Silver Nanoparticles Using Plant Seed Extracts and Their Cytotoxicity on Colorectal Cancer Cell Lines

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Abstract

In this research we focused on the green synthesis of silver nanoparticles (AgNPs) using Pimpinella anisum seed extract. Furthermore, we evaluated their cytotoxicity on colorectal cancer (CRC) cell lines. Our results revealed the anti-cancerous cytotoxic potential of green synthesized AgNPs. Green synthesized AgNPs exhibited high cytotoxicity on colorectal adenocarcinoma CRC cells. They selectively killed cancer cells through suppression of proliferation, cell cycle arrest at the G2/M phase, and induction of apoptosis. Interestingly, between the two different clones, SW620 cells were more sensitive than HCT8 cells. Overall, our findings suggest that AgNPs could be effective cancer chemotherapeutic agents or a combination nano-drug in future anti-cancer therapy.

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Acknowledgements

This project was full financially supported by King Saud University, through Vice Deanship of Research Chairs. This synthesis method has won an US patent recently (US patent issued on September 29, 2015; Publication number US 9144544 B1).

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Authors and Affiliations

  1. Research Chair in Laser Diagnosis of Cancers, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    Sandhanasamy Devanesan & Mohamad S. AlSalhi

  2. Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, 1145, Kingdom of Saudi Arabia

    Sandhanasamy Devanesan & Mohamad S. AlSalhi

  3. Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    Radhakrishnan Vishnubalaji, Nehad M. Alajez & Musaad Alfayez

  4. Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    Akram A. Alfuraydi

  5. Division of Entomology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore, Tamil Nadu, 641 046, India

    Kadarkarai Murugan

  6. Department of Zoology, EM Unit, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia

    Shaban R. M. Sayed

  7. Department of Environmental Biology, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy

    Marcello Nicoletti

  8. Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124, Pisa, Italy

    Giovanni Benelli

Authors
  1. Sandhanasamy Devanesan
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  2. Mohamad S. AlSalhi
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  3. Radhakrishnan Vishnubalaji
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  4. Akram A. Alfuraydi
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  5. Nehad M. Alajez
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  6. Musaad Alfayez
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  7. Kadarkarai Murugan
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  8. Shaban R. M. Sayed
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  9. Marcello Nicoletti
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  10. Giovanni Benelli
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Corresponding author

Correspondence to Mohamad S. AlSalhi.

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Devanesan, S., AlSalhi, M.S., Vishnubalaji, R. et al. Rapid Biological Synthesis of Silver Nanoparticles Using Plant Seed Extracts and Their Cytotoxicity on Colorectal Cancer Cell Lines. J Clust Sci 28, 595–605 (2017). https://doi.org/10.1007/s10876-016-1134-4

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  • DOI: https://doi.org/10.1007/s10876-016-1134-4

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