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Cellulose nanocrystal hybrid hydrogel with immobilized enzyme as heterogeneous biocatalyst for phenolic molecules oxidation

  • Polymers & biopolymers
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Abstract

Enzyme biocatalysts have been widely used in industrial processes; however, enzyme is difficult to separate from the reaction solution due to the homogeneous nature of biocatalyst. Hence, the cellulose nanocrystal hydrogel containing aldehyde-cellulose nanocrystal (CNCs-CHO), 2-aminoethyl methacrylate hydrochloride (AMH), itaconic acid-laccase (ITA-LAC) and N, N'-methylenebisacrylamide (MBA) as the cross-linker are synthesized using Schiff-base and APS-initiated radical polymerization, yielding the LAC-loaded heterogeneous biocatalyst. The influence of the composition on the mechanical properties, morphology, swelling behavior and permeability is investigated. The higher enzymatic activity is achieved when more CNCs are introduced into hydrogel since the incorporation of CNCs enhances permeability of hydrogel. The reusability of the LAC-loaded hybrid hydrogel is shown for 10 cycles with remaining activities of 71%. The degradation of four phenolic molecules is from 55 to 73% using Lac-loaded hybrid hydrogel as heterogeneous biocatalyst. The LAC-loaded hybrid hydrogel is used to run enzymatic reaction in a simple flow-through device which give a potential application in industry.

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Acknowledgements

The authors would like to thank Meiling Song from Shiyanjia Lab (www.shiyanjia.com) for the SEM analysis. Presented research was supported by the Xi'an Polytechnic University (Project No: 107020597), Key Research and Development Program of Shaanxi (Program No: 2021GY-212) and Scientific Research Program Funded by Shaanxi Provincial Education Department (Program No: 22JK0392).

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Authors and Affiliations

  1. School of Textile Science and Engineering, Xi’an Polytechnic University, Xi’an, 710048, Shaanxi, China

    Long Yang, Xiaofeng Peng, Simei Zhang, Guangyin Xie & Xing Zhang

  2. Key Laboratory of Functional Textile Material and Product, Xi’an Polytechnic University, Ministry of Education, Xi’an, 710048, Shaanxi, China

    Long Yang, Xiaofeng Peng, Simei Zhang, Guangyin Xie & Xing Zhang

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  1. Long Yang
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Contributions

LY contributed to methodology, writing, data curation, reviewing and editing, and project administration. XP contributed to experiment and writing. GX contributed to data curation, reviewing and editing, and project administration. XZ contributed to methodology, data curation, and project administration.

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Correspondence to Long Yang.

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Yang, L., Peng, X., Zhang, S. et al. Cellulose nanocrystal hybrid hydrogel with immobilized enzyme as heterogeneous biocatalyst for phenolic molecules oxidation. J Mater Sci 58, 6839–6849 (2023). https://doi.org/10.1007/s10853-023-08416-4

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