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Effects of sodium acetate and ammonium acetate on the growth and production of cellular components of Chlorella vulgaris 31

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Abstract

Mixotrophic cultivation using organic carbon sources has become increasingly important for large-scale production and application of microalgae, as it can overcome the current commercial bottleneck of low yield or high cost associated with photoautotrophic and heterotrophic cultivation. In this study, we investigated the effects of adding two acetate forms, sodium acetate (NaAc) and ammonium acetate (NH4Ac), at different concentrations (2, 4, 6, 8, 10 and 12 g L−1) on the biomass, biochemical components content (pigments, proteins, soluble sugars and lipids) and fatty acid composition of Chlorella vulgaris 31 (Cv-31) under mixotrophic conditions. Our results showed that the addition of NaAc significantly increased the biomass and lipid content of microalgae compared with NH4Ac, with 10 g L−1 NaAc having the best effect on the growth and lipid synthesis. Furthermore, only 2 and 4 g L−1 NaAc promoted the synthesis of pigments in algal cells, while all concentrations of NH4Ac were inhibitory. In contrast to pigments, the effect of two acetates on protein synthesis was opposite, being promoted by low concentrations of NH4Ac. Both acetates had a "low concentration promotion, high concentration inhibition" effect on the synthesis of soluble sugars. Moreover, the percentage of saturated fatty acids in the fatty acid profile increased with the amount of NaAc supplementation, while stearic acid and oleic acid appeared. Our findings suggest that regulating the type and concentration of acetate can improve the biomass and lipid yield of Cv-31 to promote the microalgal biomass production.

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Funding

This work was supported by Higher Education Industry Support Plan Project of Gansu Province, China (2020C-21; 2021CYZC-37), Key Research and Development Program of Gansu Province (22YF7NA118), Incubation Programme for Key Scientific Research Project of Northwest Normal University (NWNU-LKZD2022-02).

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  1. Shuting Peng and Yueqin Cao contributed equally to this paper.

Authors and Affiliations

  1. College of Life Sciences, Northwest Normal University, 967 Anning East Road, Lanzhou, 730070, China

    Shuting Peng, Zijian Xie, Xiaoyun Zhang, Saimai Ma & Weibao Kong

  2. School of Biotechnology, Jiangnan University, Wuxi, 214122, China

    Yueqin Cao

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  1. Shuting Peng
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Contributions

Shuting Peng: Investigation, Data curation, Methodology, Visualization, Writing-original draft, Writing-review & editing. Yueqin Cao: Investigation, Data curation, Methodology, Visualization, Writing-original draft, Writing-review & editing. Zijian Xie: Investigation, Methodology, Formal analysis, Writing-review & editing. Xiaoyun Zhang: Investigation, Methodology, Formal analysis, Writing-review & editing. Saimai Ma: Investigation, Methodology, Formal analysis, Writing-review & editing. Weibao Kong: Conceptualization, Methodology, Resources, Funding acquisition. Resources, Formal analysis, Validation, Project administration, Writing-original draft, Writing-review & editing.

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Peng, S., Cao, Y., Xie, Z. et al. Effects of sodium acetate and ammonium acetate on the growth and production of cellular components of Chlorella vulgaris 31. J Appl Phycol 36, 1–14 (2024). https://doi.org/10.1007/s10811-023-03149-y

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