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Degradation of seaweed waste by the extracellular multifunctional Amy19 enzyme and the stimulatory effects of enzymatic hydrolysates on cucumber seed germination

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Abstract

In the present study, recombinant Escherichia coli that secretes the multifunctional ɑ-amylase Amy19 was constructed and the induction conditions were optimized. The optimal induction condition obtained by orthogonal experiment was addition of 1.5% glycine for 24 h at 10 ℃. The extracellular secreted multifunctional Amy19 showed the ability to degrade seaweed (Gracilaria verrucosa) waste directly. The reducing sugar concentration increased gradually with degradation time, reaching a maximum (1.047 ± 0.0058 mg mL−1) at 60 h. HPLC showed that the higher degree of polymerization of oligosaccharides decreased gradually, while the content of galactose and 3,6-anhydro-L-galactose gradually increased with time. In the cucumber seed germination experiments, root lengths and seed germination percentages after treatment with enzymatic hydrolysates of seaweed at 5% and 10% concentrations were much greater than after treatment with water or water extracts of seaweed. This may be due to the presence of polysaccharides and oligosaccharide in EHS. In addition, seeds treated with enzymatic hydrolysates of seaweed at lower concentrations showed higher germination indexes and germination energy levels, which were associated with lower mean germination times. Thus, the use of the lower concentrations of enzymatic hydrolysates of seaweed may result in more rapid germination of seeds. This study provides important information for the efficient preparation of recombinant enzyme extracellular secretion and the agricultural applications of seaweed waste.

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Data availability

The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This research was supported by Impact and Response of Antarctic Seas to Climate Change (RFSOCC2020-2022), the National Key Research and Development Program of China (2018YFC1406704) and the Key Research and Development Program of Shandong Province, China (2018GHY115013). We thank Liwen Bianji (Edanz) (https://www.liwenbianji.cn) for editing the language of a draft of this manuscript.

Funding

Impact and Response of Antarctic Seas to Climate Change (RFSOCC2020-2022), the National Key Research and Development Program of China (2018YFC1406704) and the Key Research and Development Program of Shandong Province, China (2018GHY115013).

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Authors and Affiliations

  1. Marine Bioresource and Environment Research Center, Ministry of Natural Resources, First Institute of Oceanography, Qingdao, 266061, China

    Luying Zhao, Liping Fu, Qian Zhang & Jiang Li

  2. Key Lab of Ecological Environment Science and Technology, Ministry of Natural Resources, First Institute of Oceanography, Qingdao, 266061, China

    Luying Zhao, Liping Fu, Qian Zhang & Jiang Li

  3. CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China

    Xiaoqian Gu

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  1. Luying Zhao
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Contributions

L.Z., L.F., X.G. and Q.Z. performed the experiments. L.Z., L.F., X.G. and Q.Z. analyzed the data. L.Z., J.L. and L.F. planned and designed the research. L.Z., and J.L., wrote an initial version of the manuscript that was read and revised by all authors.

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Correspondence to Jiang Li.

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Zhao, L., Fu, L., Gu, X. et al. Degradation of seaweed waste by the extracellular multifunctional Amy19 enzyme and the stimulatory effects of enzymatic hydrolysates on cucumber seed germination. J Appl Phycol 35, 3113–3121 (2023). https://doi.org/10.1007/s10811-023-03086-w

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