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Regulation of biomass, pigments, and lipid production by Chlorella vulgaris 31 through controlling trophic modes and carbon sources

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Abstract

The effects of various carbon sources on growth, photosynthetic pigments and lipid production of Chlorella vulgaris 31 were investigated under photoautotrophic, mixotrophic and heterotrophic cultivations. The results indicated that in two concentration gradients (2 and 10 g L−1), glucose, maltose and sodium acetate all significantly promoted the growth of C. vulgaris 31, while the effects of sucrose and glycerol were not significant. The alga could barely assimilate xylose. The biosynthesis of chlorophylls and carotenoids in algal cells was reduced under mixotrophic and heterotrophic cultivations; however, lipid production was promoted significantly. Moreover, this inhibition or promotion has significant concentration dependence of carbon source. The composition and proportion of fatty acids could also be regulated by different culture conditions and organic carbon sources. The finding suggests that the biomass, pigment and lipid production of C. vulgaris 31 could be regulated by controlling the trophic mode and carbon source.

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References

  • Andrade MR, Costa JAV (2007) Mixotrophic cultivation of microalga Spirulina platensis using molasses as organic substrate. Aquaculture 264:130–134

    Google Scholar 

  • Baldisserotto C, Popovich C, Giovanardi M, Sabia A, Ferroni L, Constenla D, Leonardi P, Pancaldi S (2016) Photosynthetic aspects and lipid profiles in the mixotrophic alga Neochloris oleoabundans as useful parameters for biodiesel production. Algal Res 16:255–265

    Google Scholar 

  • Basu S, Roy AS, Mohanty K, Ghoshal AK (2013) Enhanced CO2 sequestration by a novel microalga: Scenedesmus obliquus SA1 isolated from bio-diversity hotspot region of Assam, India. Bioresour Technol 143:369–377

    CAS  PubMed  Google Scholar 

  • Cheirsilp B, Torpee S (2012) Enhanced growth and lipid production of microalgae under mixotrophic culture condition: effect of light intensity, glucose concentration and fed-batch cultivation. Bioresour Technol 110:510–516

    CAS  PubMed  Google Scholar 

  • Chen F, Chen H, Gong X (1997) Mixotrophic and heterotrophic growth of Haematococcus lacustris and rheological behavior of the cell suspensions. Bioresour Technol 62:19–24

    CAS  Google Scholar 

  • Dai C, He J, Wang G, Li S (2005) A review of ecological research on mixotrophic plankton. Acta Ecol Sin 25:2399–2405

    Google Scholar 

  • Dourou M, Aggeli D, Papanikolaou S, Aggelis G (2018) Critical steps in carbon metabolism affecting lipid accumulation and their regulation in oleaginous microorganisms. Appl Microbiol Biotechnol 102:2509–2523

    CAS  PubMed  Google Scholar 

  • Enamala MK, Enamala S, Chavali M, Donepudi J, Yadavalli R, Kolapalli B, Aradhyula TV, Velpuri J, Kuppam C (2018) Production of biofuels from microalgae-a review on cultivation, harvesting, lipid extraction, and numerous applications of microalgae. Renew Sust Energ Rev 94:49–68

    CAS  Google Scholar 

  • Gaurav N, Sivasankari S, Kiran G, Ninawe A, Selvin J (2017) Utilization of bioresources for sustainable biofuels: a review. Renew Sust Energ Rev 73:205–214

    CAS  Google Scholar 

  • GB/T 17376 (2008) Preparation of fatty acid methyl ester from animal and vegetable oils. China National Standard

  • Gong M, Bassi A (2016) Carotenoids from microalgae: a review of recent developments. Biotechnol Adv 34:1396–1412

    CAS  PubMed  Google Scholar 

  • Gupta S, Pawar SB (2019) Strategic mixed substrate cultivation of microalgae: productivity, respiration, yield, and lipid quality. J Appl Phycol 31:1573–1588

    CAS  Google Scholar 

  • Huang Y, Luo L, Xu K, Wang XC (2019) Characteristics of external carbon uptake by microalgae growth and associated effects on algal biomass composition. Bioresour Technol 292:121887

    CAS  PubMed  Google Scholar 

  • Kang RJ, Wang J, Shi DJ, Cong W, Cai ZL, Ouyang F (2004) Interactions between organic and inorganic carbon sources during mixotrophic cultivation of Synechococcus sp. Biotechnol Lett 26:1429–1432

    CAS  PubMed  Google Scholar 

  • Kim S, Park JE, Cho YB, Hwang SJ (2013) Growth rate, organic carbon and nutrient removal rates of Chlorella sorokiniana in autotrophic, heterotrophic and mixotrophic conditions. Bioresour Technol 144:8–13

    CAS  PubMed  Google Scholar 

  • Kong WB, Yang H, Cao YT, Song H, Hua SF, Xia CG (2013) Effect of glycerol and glucose on the enhancement of biomass, lipid and soluble carbohydrate production by Chlorella vulgaris in mixotrophic culture. Food Technol Biotechnol 51:62–69

    CAS  Google Scholar 

  • Kumar V, Muthuraj M, Palabhanvi B, Ghoshal AK, Das D (2014) High cell density lipid rich cultivation of a novel microalgal isolate Chlorella sorokiniana FC6 IITG in a single-stage fed-batch mode under mixotrophic condition. Bioresour Technol 170:115–124

    CAS  PubMed  Google Scholar 

  • Lari Z, Moradi-Kheibari N, Ahmadzadeh H, Abrishamchi P, Moheimani NR, Murry MA (2016) Bioprocess engineering of microalgae to optimize lipid production through nutrient management. J Appl Phycol 28:3235–3250

    CAS  Google Scholar 

  • Lari Z, Abrishamchi P, Ahmadzadeh H, Soltani N (2019) Differential carbon partitioning and fatty acid composition in mixotrophic and autotrophic cultures of a new marine isolate Tetraselmis sp. KY114885. J Appl Phycol 31:201–210

    CAS  Google Scholar 

  • Leite GB, Paranjape K, Abdelaziz AEM, Hallenbeck PC (2015) Utilization of biodiesel-derived glycerol or xylose for increased growth and lipid production by indigenous microalgae. Bioresour Technol 184:123–130

    CAS  PubMed  Google Scholar 

  • Leite GB, Paranjape K, Hallenbeck PC (2016) Breakfast of champions: fast lipid accumulation by cultures of Chlorella and Scenedesmus induced by xylose. Algal Res 16:338–348

    Google Scholar 

  • Li DJ, Wang L, Zhao QY, Wei W, Sun YH (2015) Improving high carbon dioxide tolerance and carbon dioxide fixation capability of Chlorella sp. by adaptive laboratory evolution. Bioresour Technol 185:269–275

    CAS  PubMed  Google Scholar 

  • Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembrane. Methods Enzymol 148:350–382

    CAS  Google Scholar 

  • Lin TS, Wu JY (2015) Effect of carbon sources on growth and lipid accumulation of newly isolated microalgae cultured under mixotrophic condition. Bioresour Technol 184:100–107

    CAS  PubMed  Google Scholar 

  • Liu J, Mao X, Zhou W, Guarnieri MT (2016) Simultaneous production of triacylglycerol and high-value carotenoids by the astaxanthin-producing oleaginous green microalga Chlorella zofingiensis. Bioresour Technol 214:319–327

    CAS  PubMed  Google Scholar 

  • Liu L, Zhao Y, Jiang X, Wang X, Liang W (2018) Lipid accumulation of Chlorella pyrenoidosa under mixotrophic cultivation using acetate and ammonium. Bioresour Technol 262:342–346

    CAS  PubMed  Google Scholar 

  • Lv JM, Cheng LH, Xu XH, Zhang L, Chen HL (2010) Enhanced lipid production of Chlorella vulgaris by adjustment of cultivation conditions. Bioresour Technol 101:6797–6804

    CAS  PubMed  Google Scholar 

  • Madeira M, Cardoso C, Lopes PA, Coelho D, Afonso C, Bandarra N, Prates JAM (2017) Microalgae as feed ingredients for livestock production and meat quality: a review. Livest Sci 205:111–121

    Google Scholar 

  • Manirafasha E, Ndikubwimana T, Zeng X, Lu Y, Jing K (2016) Phycobiliprotein: potential microalgae derived pharmaceutical and biological reagent. Biochem Eng J 109:282–296

    CAS  Google Scholar 

  • Menegol T, Romero-Villegas GI, López-Rodríguez M, Navarro-López E, López-Rosales L, Chisti Y, Cerón-García MC, Molina-Grima E (2019) Mixotrophic production of polyunsaturated fatty acids and carotenoids by the microalga Nannochloropsis gaditana. J Appl Phycol 31:2823–2832

    CAS  Google Scholar 

  • Mondal M, Ghosh A, Sharma AS, Tiwari ON, Gayen K, Mandal MK, Halder GN (2016) Mixotrophic cultivation of Chlorella sp. BTA 9031 and Chlamydomonas sp. BTA 9032 isolated from coal field using various carbon sources for biodiesel production. Energ Convers Manage 124:297–304

    CAS  Google Scholar 

  • Paranjape K, Leite GB, Hallenbeck PC (2016) Effect of nitrogen regime on microalgal lipid production during mixotrophic growth with glycerol. Bioresour Technol 214:778–786

    CAS  PubMed  Google Scholar 

  • Rai MP, Nigam S, Sharma R (2013) Response of growth and fatty acid compositions of Chlorella pyrenoidosa under mixotrophic cultivation with acetate and glycerol for bioenergy application. Biomass Bioenergy 58:251–257

    CAS  Google Scholar 

  • Ratledge C, Wynn JP (2002) The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms. Adv Appl Microbiol 51:1–51

    CAS  PubMed  Google Scholar 

  • Razzak SA, Ali SAM, Hossain MM, Delasa H (2017) Biological CO2 fixation with production of microalgae in wastewater–a review. Renew Sust Energ Rev 76:379–390

    CAS  Google Scholar 

  • Shin YS, Choi HI, Choi JW, Lee JS, Sung YJ, Sim SJ (2018) Multilateral approach on enhancing economic viability of lipid production from microalgae: a review. Bioresour Technol 258:335–344

    CAS  PubMed  Google Scholar 

  • Song M, Pei H (2018) The growth and lipid accumulation of Scenedesmus quadricauda during batch mixotrophic/heterotrophic cultivation using xylose as a carbon source. Bioresour Technol 263:525–531

    CAS  PubMed  Google Scholar 

  • Stadnichuk IN, Rakhimberdieva MG, Bolychevtseva YV, Yurina NP, Karapetyan NV, Selyakh IO (1998) Inhibition by glucose of chlorophyll a, and phycocyanobilin biosynthesis in the unicellular red alga Galdieria partita, at the stage of coproporphyrinogen III formation. Plant Sci 136:11–23

    CAS  Google Scholar 

  • Shen X-F, Qin Q-W, Yan S-K, Huang J-L, Liu K, Zhou S-B (2019) Biodiesel production from Chlorella vulgaris under nitrogen starvation in autotrophic, heterotrophic, and mixotrophic cultures. J Appl Phycol 31:1589–1596

    CAS  Google Scholar 

  • Stoecker DK (1998) Conceptual models of mixotrophy in planktonic protists and some ecological and evolutionary implications. Eur J Protistol 34:281–290

    Google Scholar 

  • Tan XB, Zhao XC, Yang LB, Liao JY, Zhou YY (2018) Enhanced biomass and lipid production for cultivating Chlorella pyrenoidosa in anaerobically digested starch wastewater using various carbon sources and up-scaling culture outdoors. Biochem Eng J 135:105–114

    CAS  Google Scholar 

  • Tu ZM, Liu LT, Lin WT, Xie ZZ, Luo JF (2018) Potential of using sodium bicarbonate as external carbon source to cultivate microalga in non-sterile condition. Bioresour Technol 266:109–115

    CAS  PubMed  Google Scholar 

  • Vaz BDS, Moreira JB, Morais MGD, Costa JAV (2016) Microalgae as a new source of bioactive compounds in food supplements. Curr Opin Food Sci 7:73–77

    Google Scholar 

  • Yeh KL, Chang JS (2012) Effects of cultivation conditions and media composition on cell growth and lipid productivity of indigenous microalga Chlorella vulgaris EPS-31. Bioresour Technol 105:120–127

    CAS  PubMed  Google Scholar 

  • Yeh KL, Chen CY, Chang JS (2012) pH-stat photoheterotrophic cultivation of indigenous Chlorella vulgaris ESP-31 for biomass and lipid production using acetic acid as the carbon source. Biochem Eng J 64:1–7

    CAS  Google Scholar 

  • Yu GC, Shi DJ, Cai ZL, Cong W, Ouyang F (2011) Growth and physiological features of Cyanobacterium Anabaena sp. strain PCC 7120 in a glucose mixotrophic culture. Chin J Chem Eng 19:108–115

    CAS  Google Scholar 

  • Zhang H, Wang W, Li Y, Yang W, Shen G (2011) Mixotrophic cultivation of Botryococcus braunii. Biomass Bioenergy 35:1710–1715

    CAS  Google Scholar 

  • Zhang W, Zhang P, Sun H, Chen M, Lu S, Li P (2014) Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa. Bioresour Technol 173:52–58

    CAS  PubMed  Google Scholar 

  • Zili F, Bouzidi N, Ammar J, Zakhama W, Ghoul M, Sayadi S, Ouada HB (2017) Mixotrophic cultivation promotes growth, lipid productivity, and PUFA production of a thermophilic Chlorophyta strain related to the genus Graesiella. J Appl Phycol 29:35–43

    CAS  Google Scholar 

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Funding

This work was financially supported by National Natural Science Foundation of China (No. 31360192) and Longyuan Youth Innovation & Entrepreneurship Team Project (2018).

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Authors and Affiliations

  1. College of Life Sciences, Northwest Normal University, Lanzhou, China

    Weibao Kong, Shuling Yang, Hui Wang, Huanran Huo, Baomin Guo, Na Liu, Aimei Zhang & Shiquan Niu

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  1. Weibao Kong
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  2. Shuling Yang
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  3. Hui Wang
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Correspondence to Weibao Kong.

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Kong, W., Yang, S., Wang, H. et al. Regulation of biomass, pigments, and lipid production by Chlorella vulgaris 31 through controlling trophic modes and carbon sources. J Appl Phycol 32, 1569–1579 (2020). https://doi.org/10.1007/s10811-020-02089-1

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