Abstract
Thraustochytrids, osmoheterotrophic protists which are decomposers in the marine ecosystem, are emerging as promising sources of valuable lipids such as docosahexaenoic acid and squalene. To decompose organic macromolecules in their environment, they are thought to secrete degradation enzymes. However, at present, the secretome analysis of thraustochytrids is still insufficiently studied. In this study, we attempted to identify extracellular proteins secreted by Aurantiochytrium sp. 18W-13a, a thraustochytrid strain that has high squalene content. Supernatants of cultures grown under standard growth media were concentrated and the obtained proteins were fractionated and identified using mass spectrometry. Two proteins, SP1 and SP2, were identified and found to be constitutively expressed. SP2 contains von Willebrand factor A and PAN/APPLE domains, which are involved in protein adhesion. Extracellular proteins from this strain may have roles related to protein or ligand interactions and may be necessary for the cell’s survival.
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Acknowledgements
The authors thank Prof. Makoto M. Watanabe of the Algal Biomass and Energy System Research and Development Center, University of Tsukuba for providing the Aurantiochytrium sp. 18W-13a. We also thank Dr. Masaki Yoshida from the University of Tsukuba and Dr. Ryo Koyanagi from Okinawa Institute of Science and Technology for the local protein database used in this study. The service for the Edman protein sequencing was provided by Yumiko Makino in the Functional Genomics Facility, National Institute for Basic Biology and Dr. Shoji Mano in National Institute for Basic Biology.
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Juntila, D.J., Yoneda, K. & Suzuki, I. Identification of extracellular proteins from Aurantiochytrium sp. 18W-13a. J Appl Phycol 30, 63–69 (2018). https://doi.org/10.1007/s10811-017-1171-x
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DOI: https://doi.org/10.1007/s10811-017-1171-x