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Optimisation of ultrasound-assisted extraction conditions for phenolic content and antioxidant activities of the alga Hormosira banksii using response surface methodology

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Abstract

This study aimed to optimise ultrasound-assisted extraction (UAE) conditions of the brown alga Hormosira banksii for total phenolic content (TPC) and antioxidant activities including total antioxidant capacity (ABTS), DPPH free radical scavenging capacity (DPPH) and ferric reducing antioxidant power (FRAP) using response surface methodology (RSM). Box–Behnken design was employed to assess the effect of ultrasonic temperature, time and power on the TPC and antioxidant activities of the extracts. The results showed that RSM was an accurate and reliable method in predicting TPC and antioxidant activities (ABTS, DPPH and FRAP) of the extracts with R 2 values of 0.97, 0.96, 0.92 and 0.94, respectively. The ultrasonic temperature and time had the significant impact on TPC and antioxidant capacities. The optimal UAE conditions for the maximal values of TPC and antioxidant activities were of 30 °C, 60 min and power 60%, or 150 W. The values of TPC and antioxidant activities (ABTS, DPPH, FRAP) achieved under these parameters were 23.12 (mg GAE g−1), 85.64 (mg TE g−1), 47.24 (mg TE g−1) and 12.56 (mg TE g−1), respectively. UAE was found to be more efficient in comparison to conventional extraction, with shorter time for extraction and higher of TPC level and antioxidant activities. Therefore, ultrasonic-assisted extraction using RSM is effective for extraction and further isolation and purification of phenolic compounds from H. banksii. In addition, this alga could be a potential rich source of natural antioxidants applied in food and pharmaceutical fields.

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Acknowledgements

The authors would like acknowledge the following funding support: Ramaciotti Foundation (ES2012/0104). The authors also kindly thank the University of Newcastle, Faculty of Science and IT, the Vietnamese Government through the Vietnam International Education Development - Ministry of Education and Training and the Ministry of Agriculture and Rural Development and the University of Newcastle for awarding a VIED-TUIT scholarship to Thanh Trung DANG.

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Authors and Affiliations

  1. School of Environmental and Life Sciences, Faculty of Science and Information Technology, University of Newcastle, Ourimbah, NSW, Australia

    Thanh T. Dang, Quan Van Vuong, Maria J. Schreider, Michael C. Bowyer, Ian A. Van Altena & Christopher J. Scarlett

  2. Department of Seafood Processing Technology, Faculty of Food Technology, Nha Trang University, Nha Trang, Khanh Hoa, Vietnam

    Thanh T. Dang

  3. Nutrition Food & Health Research Group, School of Environmental and Life Sciences, University of Newcastle, Brush Rd, Ourimbah, NSW, 2258, Australia

    Christopher J. Scarlett

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  1. Thanh T. Dang
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  2. Quan Van Vuong
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Correspondence to Christopher J. Scarlett.

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Dang, T.T., Van Vuong, Q., Schreider, M.J. et al. Optimisation of ultrasound-assisted extraction conditions for phenolic content and antioxidant activities of the alga Hormosira banksii using response surface methodology. J Appl Phycol 29, 3161–3173 (2017). https://doi.org/10.1007/s10811-017-1162-y

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  • DOI: https://doi.org/10.1007/s10811-017-1162-y

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