Skip to main content

Advertisement

SpringerLink
Log in

Anaerobic digestates from vinasse promote growth and lipid enrichment in Neochloris oleoabundans cultures

  • 5th Congress of the International Society for Applied Phycology
  • Published:
Journal of Applied Phycology Aims and scope Submit manuscript

Abstract

Neochloris oleoabundans (=Ettlia oleoabundans) is a green microalga that has great potential for the production of biodiesel. To achieve economically viable processes for the production of biodiesel from microalgae, the use of wastewater is highly recommended. However, there are no reports on the cultivation of N. oleoabundans utilizing anaerobic digestates of vinasse or stillage, which is a highly polluting wastewater from the alcohol industry. A first group of experiments was conducted, aiming to establish the optimal culture conditions of N. oleoabundans (UTEX 1185) using anaerobic effluents of vinasse (AEV) in bubble columns incubated under controlled conditions. The highest culture density was obtained in a medium containing 6 % of such effluents with a daily addition of sodium bicarbonate (1 g L−1). The total lipid content varied from 17.7 to 38.5 % for a range of 2 to 8 % of AEV with added sodium bicarbonate. A second group of experiments using 6 % AEV + sodium bicarbonate and flat plate photobioreactor-incubated outdoors was performed. An increase of 62 % in cell density compared to the value registered in Bold’s basal medium (BBM) was observed. Furthermore, a high ammonium–nitrogen removal (85.2 %) and a high flocculation efficiency (42 % after 30 min) indicate that dual-purpose systems aimed at producing high densities of lipid-enriched biomass of this green microalga are feasible. The uses of supplemental bicarbonate and organic waste as a source of nutrients are very important factors that contribute to reducing the cost of production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  • Abomohra A, Wagner M, El-Sheekh M, Hanelt D (2013) Lipid and total fatty acid productivity in photoautotrophic fresh water microalgae: screening studies towards biodiesel production. J Appl Phycol 25:931–936

    Article  CAS  Google Scholar 

  • APHA, American Public Health Association (1998) Methods for biomass production. In: Standard methods for the examination of water and wastewater. American Public Health Association, Baltimore

  • Cenciani K, Bittencourt-Oliveira MC, Feigl BJ, Cerri CC (2011) Sustainable production of biodiesel by microalgae and its application in agriculture. Afr J Microbiol Res 5:4638–4645

    Article  CAS  Google Scholar 

  • Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306

    Article  CAS  PubMed  Google Scholar 

  • Christenson L, Sims R (2011) Production and harvesting of microalgae for wastewater treatment, biofuels and bioproducts. Biotechnol Adv 29:686–702

    Article  CAS  PubMed  Google Scholar 

  • Elvira N, Ruíz-Marín A, Canedo-López Y (2013) Effect of nitrogen content and CO2 consumption rate by adding sodium carbonate in the lipid content of Chlorella vulgaris and Neochloris oleoabundans. Int J Environ Prot 3:13–19

    Google Scholar 

  • Emerson S, Hedges J (2008) Chemical oceanography and the marine carbon cycle. Cambridge University Press, Cambridge, pp 101–133

    Book  Google Scholar 

  • Feng D, Chen Z, Xue S, Zhang W (2011) Increased lipid production of the marine oleaginous microalgae Isochrysis zhangjiangensis (Chrysophyta) by nitrogen supplement. Bioresour Technol 102:6710–6716

    Article  CAS  PubMed  Google Scholar 

  • Franchino M, Comino E, Bona F, Riggio VA (2013) Growth of three microalgae strains and nutrient removal from an agro-zootechnical digestate. Chemosphere 92:738–744

    Article  CAS  PubMed  Google Scholar 

  • Garzón-Sanabria AJ, Ramirez-Caballero SS, Moss FEP, Nikolov ZL (2013) Effect of algogenic organic matter (AOM) and sodium chloride on Nannochloropsis salina flocculation efficiency. Bioresour Technol 143:231–237

    Article  PubMed  Google Scholar 

  • Gouveia L, Oliveira AC (2009) Microalgae as a raw material for biofuels production. J Ind Microbiol Biotechnol 36:269–274

    Article  CAS  PubMed  Google Scholar 

  • Gouveia L, Marques AE, Lopes da Silva T, Reis A (2009) Neochloris oleabundans UTEX #1185: a suitable renewable lipid source for biofuel production. J Ind Microbiol Biotechnol 36:821–826

    Article  CAS  PubMed  Google Scholar 

  • Granados MR, Acién FG, Gómez C, Fernández-Sevilla JM, Molina-Grima E (2012) Evaluation of flocculants for the recovery of freshwater microalgae. Bioresour Technol 118:102–110

    Article  CAS  PubMed  Google Scholar 

  • Joy EF, Barnard J (1975) Commercial acids and bases. In: Welcher FJ (ed) Standard methods of chemical analysis. Robert E. Krieger Publishing New York, pp 534–629

  • Klausmeier CA, Litchman E, Daufresne T, Levin SA (2004) Optimal nitrogen-to-phosphorus stoichiometry of phytoplankton. Nature 429:171–174

    Article  CAS  PubMed  Google Scholar 

  • Levine RB, Costanza-Robinson M, Spatafora GA (2011) Neochloris oleoabundans grown on anaerobically digested dairy manure for concomitant nutrient removal and biodiesel feedstock production. Biomass Bioenergy 35:40–49

    Article  CAS  Google Scholar 

  • Li Y, Horsman M, Wang B, Wu N, Lan CQ (2008) Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans. Appl Microbiol Biotechnol 81:629–636

    Article  CAS  PubMed  Google Scholar 

  • Marques SSI, Nascimento IA, de Almeida PF, Chinalia FA (2013) Growth of Chlorella vulgaris on sugarcane vinasse: the effect of anaerobic digestion pretreatment. Appl Biochem Biotechnol 171:1933–1943

    Article  CAS  PubMed  Google Scholar 

  • Moheimani NR, Borowitzka MA, Isdepsky A, Fon-Sing S (2013) Standard methods for measuring growth of algae and their composition. In: Borowitzka MA, Moheimani NR (eds) Algae for biofuels and energy. Springer, Dordrecht, pp 265–284

    Chapter  Google Scholar 

  • Murray KE, Shields JA, Garcia ND, Healy FG (2012) Productivity, carbon utilization, and energy content of mass in scalable microalgae systems. Bioresour Technol 114:499–506

    Article  CAS  PubMed  Google Scholar 

  • Nguyen TDP, Frappart M, Jaouen P, Pruvost J, Bourseau P (2014) Harvesting Chlorella vulgaris by natural increase in pH: effect of medium composition. Environ Technol 35:1378–1388

  • Olguín EJ, Doelle HW, Mercado G (1995) Resource recovery through recycling of sugar processing by-products and residuals. Resour Conserv Recycl 15:85–94

    Article  Google Scholar 

  • Olguín EJ, Galicia S, Mercado G, Pérez T (2003) Annual productivity of Spirulina (Arthrospira) and nutrient removal in a pig wastewater recycling process under tropical conditions. J Appl Phycol 15:249–257

    Article  Google Scholar 

  • Olguín EJ, Sánchez-Galván G, González-Portela RE, López-Vela M (2008) Constructed wetland mesocosms for the treatment of diluted sugarcane molasses stillage from ethanol production using Pontederia sagittata. Water Res 42:3659–3666

    Article  PubMed  Google Scholar 

  • Olguín EJ, Mercado G, Hernández ME (2011) La contaminación del agua. In: Cruz A (ed) La biodiversidad en Veracruz: estudio de estado, volumen I, Comisión Nacional para el Conocimiento y Uso de la Biodiversidad, Gobierno del Estado de Veracruz. Universidad Veracruzana, Instituto de Ecología, México, pp 369–380

    Google Scholar 

  • Olguín EJ (2012) Dual purpose microalgae-bacteria-based systems that treat wastewater and produce biodiesel and chemical products within a Biorefinery. Biotechnol Adv 30:1031–1046

    Article  PubMed  Google Scholar 

  • Olguín EJ (2013) Plant and microalgae biomass for the production of biofuels and restoration of polluted environments within a biorefinery. Invited Lecture at the 2013 World Biotechnology Congress. Boston, MA. USA Abstract Book:18

  • Olguín EJ, Castillo OS, Mendoza A, Tapia K, González-Portela RE, Hernández-Landa VJ (2015) Dual purpose system that treats anaerobic effluents from pig waste and produce Neochloris oleoabundans as lipid rich biomass. New Biotechnol. doi:10.1016/j.nbt.2014.12.004

    Google Scholar 

  • Pagnanelli F, Altimari P, Trabucco F, Toro L (2014) Mixotrophic growth of Chlorella vulgaris and Nannochloropsis oculata: interaction between glucose and nitrate. J Chem Technol Biotechnol 89:652–661

    Article  CAS  Google Scholar 

  • Park JBK, Craggs RJ, Shilton AN (2011) Wastewater treatment high rate algal ponds for biofuel production. Bioresour Technol 102:35–42

    Article  CAS  PubMed  Google Scholar 

  • Pittman JK, Dean AP, Osundeko O (2011) The potential of sustainable algal biofuel production using wastewater resources. Bioresour Technol 102:17–25

    Article  CAS  PubMed  Google Scholar 

  • Pruvost J, Van Vooren G, Le Gouic B, Couzinet-Mossion A, Legrand J (2011) Systematic investigation of biomass and lipid productivity by microalgae in photobioreactors for biodiesel application. Bioresour Technol 102:150–158

  • Rakesh S, Saxena S, Dhar DW, Prasanna R, Saxena AK (2014) Comparative evaluation of inorganic and organic amendments for their flocculation efficiency of selected microalgae. J Appl Phycol 26:399–406

    Article  CAS  Google Scholar 

  • Ritchie RJ (2006) Consistent sets of spectrophotometric chlorophyll equations for acetone, methanol and ethanol solvents. Photosynth Res 89:27–41

    Article  CAS  PubMed  Google Scholar 

  • Robles-Pliego, M., Olguín, E.J., Hernández-Landa J., González-Portela, R.E., Sánchez-Galván, G., Cuervo-López F.M. Dual purpose system for the treatment of water from a polluted river and the production of Pistia stratiotes biomass within a biorefinery. (Accepted in CLEAN – Soil, Air & Water)

  • Salim S, Vermuë MH, Wijffels RH (2012) Ratio between autoflocculating and target microalgae affects the energy-efficient harvesting by bio-flocculation. Bioresour Technol 118:49–55

    Article  CAS  PubMed  Google Scholar 

  • Sirin S, Trobajo R, Ibanez C, Salvadó J (2012) Harvesting the microalgae Phaeodactylum tricornutum with polyaluminum chloride, aluminium sulphate, chitosan and alkalinity-induced flocculation. J Appl Phycol 24:1067–1080

    Article  CAS  Google Scholar 

  • Travieso L, Benítez F, Sánchez E, Borja R, León M, Raposo F, Rincón B (2008) Assessment of microalgae pond for post-treatment of the effluent from an anaerobic fixed bed reactor treating distillery wastewater. Environ Technol 29:985–992

    Article  CAS  PubMed  Google Scholar 

  • Valderrama LT, Del Campo C, Rodríguez CM, De-Bashan LE, Bashan Y (2002) Treatment of recalcitrant wastewater from ethanol and citric acid production using the microalga Chlorella vulgaris and the macrophyte Lemna minuscula. Water Res 36:4185–4192

    Article  CAS  PubMed  Google Scholar 

  • Vandamme D, Foubert I, Fraeye I, Meesschaert B, Muylaert K (2012) Flocculation of Chlorella vulgaris induced by high pH: role of magnesium and calcium and practical implications. Bioresour Technol 105:114–119

    Article  CAS  PubMed  Google Scholar 

  • Wang B, Lan CQ (2011) Optimizing the lipid production of the green alga Neochloris oleoabundans using box-Behnken experimental design. Can J Chem Eng 89:932–939

    Article  CAS  Google Scholar 

  • Wijffels RH, Barbosa MJ (2010) An outlook on microalgal biofuels. Science 329:796–799

    Article  CAS  PubMed  Google Scholar 

  • Wu Z, Zhu Y, Huang W, Zhang C, Li T, Zhang Y, Li A (2012) Evaluation of flocculation induced by pH increase for harvesting microalgae and reuse of flocculated medium. Bioresour Technol 110:496–502

    Article  CAS  PubMed  Google Scholar 

  • Yang BJ, Zheng L, Han XT, Zheng MG (2013a) Development of TLC-FID technique for rapid screening of the chemical composition of microalgae diesel and biodiesel blends. Fuel 111:344–349

    Article  CAS  Google Scholar 

  • Yang Y, Minninberg B, Tarbet A, Weathers P (2013b) At high temperature lipid production in Ettlia oleoabundans occurs before nitrate depletion. Appl Microbiol Biotechnol 97:2263–2273

    Article  CAS  PubMed  Google Scholar 

  • Yildirim A, Demirel Z, Isleten-Hosoglu M, Akgun IH, Hatipoglu-Uslu S, Conk-Dalay M (2014) Carotenoid and fatty acid compositions of an indigenous Ettlia texensis isolate (Chlorophyceae) under phototrophic and mixotrophic conditions. Appl Biochem Biotechnol 172:1307–1319

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors acknowledge the financial support from the grant FOMIX VER-2009-C03-127097 provided by the State of Veracruz and the National Council of Science and Technology (CONACYT) and from the grant 152931 provided by the Ministry of Energy (SENER) and CONACYT, México. They also acknowledge the technical support from José Luis Domínguez Zavala.

Author information

Authors and Affiliations

  1. Institute of Ecology, Antigua carretera a Coatepec # 351, El Haya, 91190, Xalapa, Veracruz, México

    Eugenia J. Olguín, Efraín Dorantes, Omar S. Castillo & Víctor J. Hernández-Landa

Authors
  1. Eugenia J. Olguín
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Efraín Dorantes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Omar S. Castillo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Víctor J. Hernández-Landa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Eugenia J. Olguín.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Olguín, E.J., Dorantes, E., Castillo, O.S. et al. Anaerobic digestates from vinasse promote growth and lipid enrichment in Neochloris oleoabundans cultures. J Appl Phycol 27, 1813–1822 (2015). https://doi.org/10.1007/s10811-015-0540-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10811-015-0540-6

Keywords

Search

Navigation