Abstract
The γ-aminobutyric acid is a non-protein amino acid with a so far unestablished role in Liriodendron hybrid somatic embryogenesis. In this study, we aimed to establish a rapid somatic embryo induction methodology, and to this end we investigated how GABA affects somatic embryo induction and development in Liriodendron hybrid. We successfully induced somatic embryos using embryogenic callus treated with different GABA concentrations. The addition of 6 mg/L GABA to MS basic medium showed a positive effect on the induction and maturation of somatic embryos, which had no effect on the structure of somatic embryos. GABA stimulated higher expression of LhWUS, LhCLV3 and LhWOX2 and an earlier LhCLV3 expression peak. In addition, we found that GABA affected the plant morphological growth, changing the number of lateral roots, root length and stem diameter of plantlets germinated from somatic embryos of Liriodendron hybrid. However, there was no significant effect on plant height and leaf number. Our research may provide benefits for the improvement of somatic embryogenesis protocols by using GABA, which lays a good foundation to study the molecular mechanism that GABA regulates somatic embryo induction and development.
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Abbreviations
- GABA:
-
γ-Aminobutyric acid
- SE:
-
Somatic embryogenesis
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Funding
This work was supported by Nature Science Foundation of China (32071784, 31770715), the Key Research and Development Plan of Jiangsu Province (BE2017376), the Qinglan Project of Jiangsu Province, Distinguished Professor Project of Jiangsu province and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
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CJH and SJS contributed conception and design of the study; CTT, YDJ and FRF performed the experiments; CTT, YDJ, FRF, ZRH, LY and CTL carried out the statistical analysis; CTT wrote the manuscript. All authors contributed to manuscript revision, read, and approved the submitted version.
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Communicated by Radomira Vankova.
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Chen, T., Yang, D., Fan, R. et al. γ-Aminobutyric acid a novel candidate for rapid induction in somatic embryogenesis of Liriodendron hybrid. Plant Growth Regul 96, 293–302 (2022). https://doi.org/10.1007/s10725-021-00776-8
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DOI: https://doi.org/10.1007/s10725-021-00776-8