Abstract
Astyanax lacustris, locally known as lambari-do-rabo-amarelo, is a study model for Neotropical fish. Testis of A. lacustris shows deep morphophysiological changes throughout the annual reproductive cycle. This work analyzed the distribution of claudin-1, actin, and cytokeratin as elements of the cytoskeleton in germinal epithelium and interstitium; the distribution of type I collagen, fibronectin, and laminin as extracellular matrix compounds; and the localization of androgen receptor in the testis of this species. Claudin-1, cytokeratin, and actin were present in the Sertoli cells and modified Sertoli cells, and actin was also detected in peritubular myoid cells. Type I collagen were in the interstitial tissue, laminin in the basement membrane of germinal epithelium and endothelium, but fibronectin was additionally detected in the germinal epithelium compartment. The labeling of androgen receptor was higher in peritubular myoid cells and undifferentiated spermatogonia, and weaker labeling was detected in type B spermatogonia. Therefore, the present work highlights new aspects of the biology of the testis of A. lacustris, and contribute to amplify the understanding of this organ.
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Fabiano Gonçalves Costa: Responsible for the conduction of all steps of the research; Outlined the research project. Chayrra Chehade Gomes and Mateus Contar Adolfi: Processed the samples, and produce the micrographies. Mayra Costa da Cruz Gallo de Carvalho, Marco Antônio Zanoni and Fábio Rodrigues Ferreira Seiva: Help in the immunohistochemical assays. Maria Inês Borella: Advisor; Co-responsible for conception of the research project; Mentoring during the execution of the research and she is the research leader. All of the authors reviewed the manuscript.
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Costa, F.G., Gomes, C.C., Adolfi, M.C. et al. New approaches concerning the testis of Astyanax lacustris (Characidae): immunohistochemical studies. Fish Physiol Biochem 49, 543–556 (2023). https://doi.org/10.1007/s10695-023-01194-5
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DOI: https://doi.org/10.1007/s10695-023-01194-5