Abstract
cMyc is a vital transcription factor that involves in the regulation of cell proliferation, growth, differentiation, and apoptosis. In the present study, cMyc in Larimichthys crocea (Lc-cMyc) was cloned and analyzed for investigating its function. The full-length cDNA of Lc-cMyc was 2089 bp encoding a 440-amino-acid protein (Lc-cMyc). Lc-cMyc had the characteristic helix-loop-helix-leucine-zipper (HLH-LZ) DNA-binding domain and highly conservative in evolution. The expression of Lc-cMyc was detected by quantitative real-time PCR (qRT-PCR) and in situ hybridization, respectively. In tissues, the gender differences of Lc-cMyc expression existed only in gonad and Lc-cMyc was extremely significantly expressed in ovary with the highest level in 635-dph ovary, especially in stages II (late) and III (early) oocytes. A certain degree of expression was examined in head kidney of both sexes and testis with high expression in spermatocyte. In embryos, Lc-cMyc was expressed at all embryonic stages. In early embryogenesis (from two-cell stage to mutiple-cell stage), Lc-cMyc was expressed very highly with a peak at two-cell stage. In late embryogenesis (from blastula stage to 1-day-post-hatching stage), the high expression of Lc-cMyc was detected as the following order: 1-day-post-hatching stage > pre-hatching stage > the appearance-of-optic-vesicles stage = mutiple-cell stage > beginning-of-heart-pulsation stage. The distribution of Lc-cMyc concentrated gradually in the back of embryos until in the head. In conclusion, the spatio-temporal expression patterns of Lc-cMyc indicated an essential role in oogenesis and embryogenesis and contributed to insight into the molecular mechanisms of regulating pluripotency in large yellow croaker.
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Acknowledgments
The work was supported by the National Natural Science Foundation of China (No. 41306174), the program of the Science and Technology Department of Fujian Province (No. 2013N0024), the start up fund of Jimei University (ZQ2017003), and the Innovation Team Foundation of Jimei University (No. 2010A001).
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Jiang, Y., Han, K., Chen, S. et al. Isolation, characterization, and expression of proto-oncogene cMyc in large yellow croaker Larimichthys crocea . Fish Physiol Biochem 43, 1443–1461 (2017). https://doi.org/10.1007/s10695-017-0384-x
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DOI: https://doi.org/10.1007/s10695-017-0384-x