Abstract
The soybean mosaic virus (SMV) is a viral pathogen caused by Potyvirus reported in the major soybean growing areas of the world. SMV strain SC-15 is the most virulent and predominant strain that infects all soybean differentials and is widely distributed throughout southern China. In this study, three elite SMV-resistant soybean varieties that possess better comprehensive traits were identified through a screening test. A mapping population containing 163 F8 recombinant inbred lines derived from a Zhonghuang24 (resistant) × Huaxia3 (susceptible) cross was used to locate the resistance gene for SMV strain SC-15. A high-density genetic linkage map was developed containing 2639 recombination bins using the restriction site-associated DNA sequencing (RAD-seq) method. Results revealed a novel resistance gene, RSC15ZH identified in a 63 kb region of chromosome 13. This locus was further characterized by quantitative real-time PCR (qRT-PCR). Four candidate resistance genes were identified that were possibly involved in regulating SMV resistance, especially Glyma13g24461. These results provide a basic foundation for soybean breeding programs to improve resistance to SMV strain SC-15 and for positional cloning of SMV resistant candidate genes.
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Acknowledgements
We wish to thank Professor Haijian Zhi (Nanjing Agricultural University, People’s Republic of China) for kindly supplying the SMV of SC-15 strain and the susceptible soybean accession Nannong1138-2. We also thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript. This work was supported by the Science and Technology Projects of Guangzhou City (201804020015); the China Agricultural Research System (CARS-04-PS09) and the Research Project of the State Key Laboratory of Agricultural and Biological Resources Protection and Utilization in Subtropics.
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ML and HN designed and directed the project. ML, NL, and QM participated in the analysis of phenotypic data. TL and ZC helped perform experiments. TL and QM was responsible for mapping the QTLs. ML and ZC performed the qRT-PCR analysis. ML and NL wrote the manuscript with input from all authors. NL designed the figures and tables in the manuscript. HN helped supervise the project. All authors discussed the results and contributed to the final manuscript.
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Li, M., Liu, N., Ma, Q. et al. Fine mapping and analyses of the RSC15ZH resistance candidate gene for the soybean mosaic virus. Euphytica 216, 47 (2020). https://doi.org/10.1007/s10681-020-02581-2
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DOI: https://doi.org/10.1007/s10681-020-02581-2