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Fine mapping of the R SC14Q locus for resistance to soybean mosaic virus in soybean

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Abstract

Soybean mosaic virus (SMV) disease is one of the most destructive soybean (Glycine max (L.) Merr.) diseases worldwide, causing yield losses and significant deterioration in seed quality. ‘Qihuang No.1’ has a wide resistance spectrum and is extensively used in breeding programs in China. The dominant SMV resistance gene R SC14Q from the cultivar Qihuang No.1 was previously mapped to a 4.2 cM region on soybean chromosome 13 (formerly linkage group (LG) F). Fine mapping of R SC14Q is essential for high efficiency of marker-assisted selection (MAS) and its map-based cloning. Residual heterozygous lines (RHLs) derived from a soybean recombinant inbred line (RIL) were used to fine map R SC14Q . The RILs were obtained from a cross between ‘Qihuang No.1’ (resistant) and ‘Nannong1138-2’ (susceptible). The genomic sequence in an approximately 6 cM interval between the markers Satt334 and Sct_033 flanking R SC14Q was used to develop five simple sequence repeats and two InDel markers. Linkage analysis between inoculation phenotype and genetic markers localized R SC14Q to the interval between Satt334 and MY750, with genetic distances of 0.6 and 0.5 cM, respectively, corresponding to a physical distance on the ‘Williams 82’ draft assembly (Glyma1.13) of 1.18 Mb. Based on the genes sequences in the R SC14Q locus region with predicted functions for resistance, one SNP marker, MY525, was developed between Satt334 and MY750. Thus, the genomic region containing the R SC14Q was further narrowed to a 616 kb interval.

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Acknowledgments

This work was supported by the State Key Basic Research and Development Plan of China (Grant No. 2006A10A111), the National Natural Science Foundation of China (Grant No. 30971815) and the MOE 111 Project (Grant No. B08025). We also thank Song Q.J. from Soybean Genomics and Improvement Laboratory, USDA-ARS for providing the SSR markers primer sequences.

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Authors and Affiliations

  1. National Key Laboratory for Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement of Nanjing Agriculture University, Nanjing, 210095, People’s Republic of China

    Ying Ma, Da-Gang Wang, Hai-Chao Li, Gui-Jie Zheng, Yong-Qing Yang, Hua-Wei Li & Hai-Jian Zhi

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  1. Ying Ma
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  2. Da-Gang Wang
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  3. Hai-Chao Li
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Correspondence to Hai-Jian Zhi.

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Ma, Y., Wang, DG., Li, HC. et al. Fine mapping of the R SC14Q locus for resistance to soybean mosaic virus in soybean. Euphytica 181, 127–135 (2011). https://doi.org/10.1007/s10681-011-0457-3

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  • DOI: https://doi.org/10.1007/s10681-011-0457-3

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