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Genome-wide SNP-based association mapping of resistance to Phytophthora sojae in soybean (Glycine max (L.) Merr.)

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Abstract

Phytophthora root rot (PRR) is among the most important soybean (Glycine max (L.) Merr.) diseases worldwide, and the host displays complex genetic resistance. A genome-wide association study was performed on 337 accessions from the Yangtze-Huai soybean breeding germplasm to identify resistance regions associated with PRR resistance using 60,862 high-quality single nucleotide polymorphisms markers. Twenty-six significant SNP-trait associations were detected on chromosomes 01 using a mixed linear model with the Q matrix and K matrix as covariates. In addition, twenty-six SNPs belonged to three adjacent haplotype blocks according to a linkage disequilibrium blocks analysis, and no previous studies have reported resistance loci in this 441 kb region. The real-time RT-PCR analysis of the possible candidate genes showed that two genes (Glyma01g32800 and Glyma01g32855) are likely involved in PRR resistance. Markers associated with resistance can contribute to marker-assisted selection in breeding programs. Analyses of candidate genes can lay a foundation for exploring the mechanism of P. sojae resistance.

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Acknowledgements

We thank Prof. Yuanchao Wang (Nanjing Agricultural University) for providing the isolates of P. sojae. This work was supported by National Key R&D Program of China (2017YFD0101500), Modern Agro-industry Technology Research System of China (CARS-04-PS10), Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT_17R55), Jiangsu Collaborative Innovation Center for Modern Crop Production, The Natural Science Foundation of China (31571691) and Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX17_0560).

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Authors and Affiliations

  1. National Center for Soybean Improvement, Key Laboratory of Biology and Genetics and Breeding for Soybean, Ministry of Agriculture, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, No.1 Weigang, Nanjing, Jiangsu, 210095, People’s Republic of China

    Jingping Niu, Na Guo, Zhang Zhang, Zili Wang, Jianli Huang, Jinming Zhao, Fangguo Chang, Haitang Wang, Tuanjie Zhao & Han Xing

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  1. Jingping Niu
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  2. Na Guo
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  3. Zhang Zhang
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  4. Zili Wang
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  5. Jianli Huang
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  6. Jinming Zhao
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  7. Fangguo Chang
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  8. Haitang Wang
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  9. Tuanjie Zhao
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  10. Han Xing
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Correspondence to Tuanjie Zhao or Han Xing.

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Niu, J., Guo, N., Zhang, Z. et al. Genome-wide SNP-based association mapping of resistance to Phytophthora sojae in soybean (Glycine max (L.) Merr.). Euphytica 214, 187 (2018). https://doi.org/10.1007/s10681-018-2262-8

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