Skip to main content
SpringerLink
Log in

Identification and molecular mapping of Rps11, a novel gene conferring resistance to Phytophthora sojae in soybean

  • Original Article
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Key message

Rps11 confers excellent resistance to predominant Phytophthora sojae isolates capable of defeating major Rps genes deployed into soybean production, representing a novel source of resistance for soybean cultivar enhancement.

Abstract

Phytophthora root and stem rot (PRSR), caused by the soil-borne pathogen Phytophthora sojae, is a devastating disease of soybean [Glycine max (L.) Merr.] throughout the world. Deploying resistant soybean cultivars is the most effective and environmentally friendly approach to managing this disease. The soybean landrace PI 594527 was found to carry excellent resistance to all P. sojae isolates examined, some of which were capable of overcoming the major Rps genesp, such as Rps1-k, Rps1-c, and Rps3-a, predominantly used for soybean protection in the past decades. A mapping population consisting of 58 F2 individuals and 209 F2:3 families derived from a cross between PI 594527 and the susceptible cultivar ‘Williams’ was used to characterize the inheritance pattern of the resistance to P. soja (Rps) in PI 594527. It was found that the resistance was conferred by a single Rps gene, designated Rps11, which was initially defined as an ~5 Mb genomic region at the beginning of chromosome 7 by bulked segregant analysis (BSA) with a nucleotide polymorphism (SNP) chip comprising 7039 SNP markers. Subsequently, simple sequence repeat (SSR) markers in the defined region were used to genotype the F2:3 mapping population to map Rps11 to a 225.3 kb genomic region flanked by SSR markers BARCSOYSSR_07_0286 and BARCSOYSSR_07_0300, according to the soybean reference genome sequence. Particularly, an SSR marker (i.e., BARCSOYSSR_07_0295) was found to tightly co-segregate with Rps11 in the mapping population and can be effectively used for marker-assisted selection of this gene for development of resistant soybean cultivars.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Allen G, Flores-Vergara M, Krasynanski S, Kumar S, Thompson W (2006) A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide. Nat Protoc 1:2320–2325

    Article  PubMed  CAS  Google Scholar 

  • Demirbas A et al (2001) Simple sequence repeat markers linked to the soybean genes for Phytophthora resistance. Crop Sci 41:1220–1227

    Article  CAS  Google Scholar 

  • Dorrance A, Schmitthenner A (2000) New sources of resistance to Phytophthora sojae in the soybean plant introductions. Plant Dis 84:1303–1308

    Article  CAS  Google Scholar 

  • Dorrance A, Mills D, Robertson A, Draper M, Giesler L, Tenuta A (2007) Phytophthora root and stem rot of soybean. Plant Health Instr. doi:10.1094/PHI-I-2007-0830-07

    Google Scholar 

  • Dorrance AE, Berry SA, Anderson TR, Meharg C (2008) Isolation, storage, pathotype characterization, and evaluation of resistance for Phytophthora sojae in soybean. Plant Health Prog. doi:10.1094/PHP-2008-0118-01-DG

    Google Scholar 

  • Du J, Tian Z, Sui Y, Zhao M, Song Q, Cannon SB, Cregan P, Ma J (2012) Pericentromeric effects shape the patterns of divergence, retention, and expression of duplicated genes in the palaeopolyploid soybean (Glycine max (L.). Plant Cell 24:21–32

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Erwin DC, Ribeiro OK (1996) Phytophthora diseases worldwide. American Phytopathological Society (APS Press), St Paul

    Google Scholar 

  • Fan A, Wang X, Fang X, Wu X, Zhu Z (2009) Molecular identification of Phytophthora resistance gene in soybean cultivar Yudou 25. Acta Agron Sinica 35:1844–1850

    Article  CAS  Google Scholar 

  • Gao H, Narayanan NN, Ellison L, Bhattacharyya MK (2005) Two classes of highly similar coiled coil-nucleotide binding-leucine rich repeat genes isolated from the Rps1-k locus encode Phytophthora resistance in soybean. Mol Plant Microbe Interact 18:1035–1045

    Article  PubMed  CAS  Google Scholar 

  • Gordon SG, Martin SKS, Dorrance AE (2006) Rps8 maps to a resistance gene rich region on soybean molecular linkage group F. Crop Sci 46:168–173

    Article  CAS  Google Scholar 

  • Kanazin V, Marek LF, Shoemaker RC (1996) Resistance gene analogs are conserved and clustered in soybean. Proc Natl Acad Sci USA 93:11746–11750

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Kasuga T, Gijzen M (2013) Epigenetics and the evolution of virulence. Trends Microbiol 21:575–582

    Article  PubMed  CAS  Google Scholar 

  • Kaufmann MJ, Gerdemann J (1958) Root and stem rot of soybean caused by Phytophthora sojae n. sp. Phytopathology 48:201–208

    Google Scholar 

  • Koenning SR, Wrather JA (2010) Suppression of soybean yield potential in the continental United States by plant diseases from 2006 to 2009. Plant Health Prog. doi:10.1094/PHP-2010-1122-01-RS

    Google Scholar 

  • Lin F et al (2013) Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B. Theor Appl Genet 126:2177–2185

    Article  PubMed  CAS  Google Scholar 

  • McDonald BA, Linde C (2002) Pathogen population genetics, evolutionary potential, and durable resistance. Annu Rev Phytopathol 40:349–379

    Article  PubMed  CAS  Google Scholar 

  • Michelmore RW, Paran I, Kesseli R (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Na R, Yu D, Qutob D, Zhao J, Gijzen M (2013) Deletion of the Phytophthora sojae avirulence gene Avr1d causes gain of virulence on Rps1d. Mol Plant Microbe Interact 26:969–976

    Article  PubMed  CAS  Google Scholar 

  • Ping J et al (2014) Dt2 is a gain-of-function MADS-domain factor gene that specifies semideterminacy in soybean. Plant Cell 26:2831–2842

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Polzin K, Lohnes D, Nickell C, Shoemaker R (1994) Integration of Rps2, Rmd, and Rj2 into linkage group J of the soybean molecular map. J Hered 85:300–303

    CAS  Google Scholar 

  • Qutob D, Chapman BP, Gijzen M (2013) Transgenerational gene silencing causes gain of virulence in a plant pathogen. Nat Commun 4:1349

    Article  PubMed  PubMed Central  Google Scholar 

  • Sandhu D, Gao H, Cianzio S, Bhattacharyya MK (2004) Deletion of a disease resistance nucleotide-binding-site leucine-rich-repeat-like sequence is associated with the loss of the Phytophthora resistance gene Rps4 in soybean. Genetics 168:2157–2167

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Schmitthenner AF (1985) Problems and progress in control of phytophthora root-rot of soybean. Plant Dis 69:362–368

    Article  Google Scholar 

  • Schmitthenner A (1999) Phytophthora rot of soybean. In: Hartman GL, Sinclair JB, Rupe JC (eds) Compendium of soybean diseases, 4th edn. The American Phytopathological Society (APS) Press, St Paul, pp 39–42

    Google Scholar 

  • Schmutz J et al (2010) Genome sequence of the palaeopolyploid soybean. Nature 463:178–183

    Article  PubMed  CAS  Google Scholar 

  • Song Q et al (2010) Abundance of SSR motifs and development of candidate polymorphic SSR markers (BARCSOYSSR_1.0) in soybean. Crop Sci 50:1950–1960

    Article  CAS  Google Scholar 

  • Song Q, Hyten DL, Jia G, Quigley CV, Fickus EW, Nelson RL, Cregan PB (2013) Development and evaluation of SoySNP50K, a high-density genotyping array for soybean. PLoS One 8:e54985

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Song Q, Hyten DL, Jia G, Quigley CV, Fickus EW, Nelson RL, Cregan PB (2015) Fingerprinting soybean germplasm and its utility in genomic research. G3 Genes| Genomes Genetics 5:1999–2006

    Article  PubMed  PubMed Central  Google Scholar 

  • Sugimoto T et al (2011) Genetic analysis and identification of DNA markers linked to a novel Phytophthora sojae resistance gene in the Japanese soybean cultivar Waseshiroge. Euphytica 182:133–145

    Article  CAS  Google Scholar 

  • Sun S et al (2011) Characterization and mapping of RpsYu25, a novel resistance gene to Phytophthora sojae. Plant Breed 130:139–143

    Article  CAS  Google Scholar 

  • Sun J, Li L, Zhao J, Huang J, Yan Q, Xing H, Guo N (2014) Genetic analysis and fine mapping of RpsJS, a novel resistance gene to Phytophthora sojae in soybean [Glycine max (L.) Merr.]. Theor Appl Genet 127:913–919

    Article  PubMed  CAS  Google Scholar 

  • Van Ooijen J (2011) Multipoint maximum likelihood mapping in a full-sib family of an outbreeding species. Genet Res 93:343–349

    Article  Google Scholar 

  • Weng C, Yu K, Anderson T, Poysa V (2001) Mapping genes conferring resistance to Phytophthora root rot of soybean, Rps1a and Rps7. J Hered 92:442–446

    Article  PubMed  CAS  Google Scholar 

  • Wrather J, Koenning S (2009) Effects of diseases on soybean yields in the United States 1996 to 2007. Plant Health Prog. doi:10.1094/PHP-2009-0401-01-RS

    Google Scholar 

  • Wu X et al (2011a) Identification, genetic analysis and mapping of resistance to Phytophthora sojae of Pm28 in soybean. Agr Sci China 10:1506–1511

    Article  CAS  Google Scholar 

  • Wu X, Zhou B, Sun S, Zhao J, Chen S, Gai J, Xing H (2011b) Genetic analysis and mapping of resistance to phytophthora sojae of Pm14 in soybean. Sci Agric Sinica 44:456–460

    Google Scholar 

  • Yao H, Wang X, Wu X, Xiao Y, Zhu Z (2010) Molecular mapping of Phytophthora resistance gene in soybean cultivar zaoshu18. J Plant Genet Res 11:213–217

    CAS  Google Scholar 

  • Zhang X, Feng Y, Cheng H, Tian D, Yang S, Chen J-Q (2011) Relative evolutionary rates of NBS-encoding genes revealed by soybean segmental duplication. Mol Genet Genomics 285:79–90

    Article  PubMed  CAS  Google Scholar 

  • Zhang J, Xia C, Wang X, Duan C, Sun S, Wu X, Zhu Z (2013) Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar. Theor Appl Genet 126:1555–1561

    Article  PubMed  CAS  Google Scholar 

  • Zhu Z, Huo Y, Wang X, Huang J, Wu X (2007) Molecular identification of a novel Phytophthora resistance gene in soybean. Acta Agron Sinica 33:154–157

    CAS  Google Scholar 

Download references

Acknowledgments

This work was mainly supported by Dow AgroSciences LLC, and partially supported by Indiana Soybean Alliance, the “Partnership for Research & Education in Plant Breeding and Genetics” program funded by the U.S. Department of Agriculture’s National Institute of Food and Agriculture, and corporate partners Ag Alumni Seed, AgReliant Genetics, Beck’s Hybrids, ConAgraFoods, Dow AgroSciences, Indiana Crop Improvement Association and Pioneer Hi-Bred International, and Indiana Soybean Alliance. We would like to thank Teresa J. Hughes for assistance with pathogen preparation and inoculation.

Author information

Author notes

  1. Feng Lin

    Present address: Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, 48824, USA

  2. Jieqing Ping

    Present address: Bayer CropScience LP, Research Triangle Park, NC, 27709, USA

Authors and Affiliations

  1. Department of Agronomy, Purdue University, West Lafayette, IN, 47907, USA

    Jieqing Ping, Joshua C. Fitzgerald, Chunbao Zhang, Feng Lin & Jianxin Ma

  2. Dow AgroSciences LLC., Indianapolis, IN, 46268, USA

    Yonghe Bai, Rajat Aggarwal, Maqsood Rehman & Oswald Crasta

  3. Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI, 48824, USA

    Dechun Wang

  4. Bayer CropScience LP, Research Triangle Park, NC, 27709, USA

    Yonghe Bai

Authors
  1. Jieqing Ping
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Joshua C. Fitzgerald
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chunbao Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Feng Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yonghe Bai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Dechun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Rajat Aggarwal
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Maqsood Rehman
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Oswald Crasta
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Jianxin Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jianxin Ma.

Ethics declarations

Conflict of interest

This work was filed for a US Provisional Patent Application (No. 62/170,441) on June 2, 2015 by Dow AgroSciences LLC.

Additional information

Communicated by I. Rajcan.

Jieqing Ping, Joshua C. Fitzgerald and Chunbao Zhang contributed equally to this work.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ping, J., Fitzgerald, J.C., Zhang, C. et al. Identification and molecular mapping of Rps11, a novel gene conferring resistance to Phytophthora sojae in soybean. Theor Appl Genet 129, 445–451 (2016). https://doi.org/10.1007/s00122-015-2638-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-015-2638-2

Keywords

Search

Navigation