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Population structure and association mapping of traits related to reproductive development in field pea

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Abstract

Field pea (Pisum sativum) is an important pulse crop globally for human consumption and livestock feed. A panel of 92 diverse pea cultivars was evaluated across nine environments and genotyped using 1536 single nucleotide polymorphisms (SNPs) arranged in a GoldenGate array. Population structure analysis revealed three subpopulations roughly consistent with the cultivar origin. Phenotyping included days to flowering (DTF), duration of flowering (DOF), number of reproductive nodes, number of pods on the main stem, percentage of pods set, percentage of pods retained with seed and pollen germination reduction due to heat stress. Association analyses identified a total of 60 SNPs significantly associated (−log10 p ≥ 4.3) with these seven reproductive development-related traits. Among these 60 marker-trait associations, 33 SNPs were associated with the onset of flowering, 8 SNPs with pod development and 19 SNPs with the number of reproductive nodes. No SNP marker was significantly associated with in vitro pollen germination reduction caused by high temperature stress. We found that 12 SNPs associated with DTF and 2 SNPs associated with DOF overlapped with the SNP markers associated with the number of reproductive nodes. Genomic regions associated with variation for reproductive development-related traits identified in this study provide grounds for future genetic improvement in pea.

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Acknowledgements

This work was supported by Saskatchewan Agriculture Development Fund [Grant Number 20100033], Saskatchewan Pulse Growers Association [Grant Number AGR1116], Western Grains Research Fund [Grant Number 411939], and the Natural Sciences and Engineering Research Council (NSERC) of Canada—Collaborative Research and Development (CRD) Program [Grant Number CRDPJ 439277]. We acknowledge the kind help from the staff at the Pulse Crop Breeding Program of the Crop Science Field Laboratory and the staff at the Crop Physiology Lab at University of Saskatchewan. We thank Amidou N’Diaye and Kirstin Bett for helpful discussions. We are grateful for the helpful comments from two anonymous referees that improved this manuscript.

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  1. Marwan Diapari

    Present address: Genomics and Biotechnology, London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada

Authors and Affiliations

  1. Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada

    Yunfei Jiang, Marwan Diapari, Rosalind A. Bueckert, Bunyamin Tar’an & Thomas D. Warkentin

  2. Crop Development Centre, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK, S7N 5A8, Canada

    Marwan Diapari, Bunyamin Tar’an & Thomas D. Warkentin

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  1. Yunfei Jiang
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Correspondence to Thomas D. Warkentin.

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Y. Jiang and M. Diapari are joint first authors.

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Jiang, Y., Diapari, M., Bueckert, R.A. et al. Population structure and association mapping of traits related to reproductive development in field pea. Euphytica 213, 215 (2017). https://doi.org/10.1007/s10681-017-2006-1

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