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Induction of 2n pollen with colchicine during microsporogenesis in Eucalyptus

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Abstract

Artificially inducing 2n gametes through chromosome doubling is an effective way to obtain polyploids. In this study, Eucalyptus urophylla microsporogenesis and flower development were investigated to guide 2n pollen induction. We also investigated suitable conditions for colchicine treatment. Our results showed that E. urophylla 2n pollen was spherical and had a large volume (mean diameter 28.57 ± 0.46 μm), while normal untreated pollen (mean diameter 19.68 ± 0.11 μm) was tetrahedron. The highest rate of 2n pollen production was 28.71 % when the flower buds, which ranged in size from 3.5 to 4.0 mm, underwent treatment with 0.5 % colchicine solution for 6 h. Further studies suggested that diplotene to diakinesis and metaphase I to telophase I were suitable meiotic stages for chromosomes doubling, due to asynchronous development of microsporogenesis between the anthers in a single flower bud. These data help to illuminate research in other areas, such as triploid eucalyptus production by chromosome doubling of female gametes.

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Acknowledgments

This research was financially supported by the Special Fund for Forest Scientific Research in the Public Welfare (201404113), the 111 Project (B13007) and the Program for Changjiang Scholars and Innovative Research Team in University (IRT13047). The authors would like to thank Jun Lan and Donglin Chen from Guangxi Dongmen Forest Farm for additional help.

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Authors and Affiliations

  1. National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Jun Yang, Pengqiang Yao, Yun Li & Xiangyang Kang

  2. Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Jun Yang, Pengqiang Yao, Yun Li & Xiangyang Kang

  3. Guangxi Dongmen Forest Farm, Guangxi Zhuang Autonomous Region, 532108, People’s Republic of China

    Jiyou Mo & Jianzhong Wang

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  1. Jun Yang
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  2. Pengqiang Yao
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  3. Yun Li
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Correspondence to Xiangyang Kang.

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Yang, J., Yao, P., Li, Y. et al. Induction of 2n pollen with colchicine during microsporogenesis in Eucalyptus . Euphytica 210, 69–78 (2016). https://doi.org/10.1007/s10681-016-1699-x

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