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Induction of 2n pollen formation in Begonia by trifluralin and N2O treatments

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Abstract

In this study, treatments of both trifluralin (at 10, 100 and 1000 μM) and N2O (in the form of gas under pressure) were applied to Begonia flower buds to induce the formation of 2n pollen. Three male fertile species (B. cucullata, B. subvillosa var. leptotricha and B. fischeri) and two male sterile hybrids (B. schmidtiana × B. cucullata and B. subvillosa var. leptotricha × B. cucullata) were treated. Pollen size, which is related to pollen DNA content, increased after both N2O and trifluralin treatments, but the induction of large pollen was genotype dependent. Trifluralin induced large pollen only in the male fertile species, while N2O treatments induced fertile 2n pollen in the male sterile B. schmidtiana × B. cucullata. Cytological studies showed that trifluralin induced multinuclear monads that resulted in 4n gametes in stead of 2n gametes. In general, large pollen obtained after trifluralin treatments showed low germination capability, while large pollen obtained after N2O treatments retained high germination capability. Seedlings with raised ploidy level could only be obtained after crosses were performed with large pollen obtained from N2O treatments. Hence, N2O treatments are preferable to the use of trifluralin to induce 2n gametes in Begonia.

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Acknowledgements

The authors want to thank the Institute for Agricultural and Fisheries Research (ILVO) and IWT-Flanders (project 40696) for their financial support.

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Authors and Affiliations

  1. Plant Sciences Unit, Institute for Agricultural and Fisheries Research (ILVO), Caritasstraat 21, 9090, Melle, Belgium

    Angelo Dewitte, Tom Eeckhaut, Johan Van Huylenbroeck & Erik Van Bockstaele

  2. Department of Health Care and Biotechnology, KATHO Catholic University College of South-West Flanders, Wilgenstraat 32, 8800, Roeselare, Belgium

    Angelo Dewitte

  3. Faculty of Bioscience Engineering, Department of Plant Production, Ghent University, Coupure Links 653, 9000, Ghent, Belgium

    Erik Van Bockstaele

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  1. Angelo Dewitte
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  2. Tom Eeckhaut
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Correspondence to Angelo Dewitte.

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Dewitte, A., Eeckhaut, T., Van Huylenbroeck, J. et al. Induction of 2n pollen formation in Begonia by trifluralin and N2O treatments. Euphytica 171, 283–293 (2010). https://doi.org/10.1007/s10681-009-0060-z

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  • DOI: https://doi.org/10.1007/s10681-009-0060-z

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