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Induction of unreduced megaspores with high temperature during megasporogenesis in Populus

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Abstract

Introduction

Triploid breeding is one of the most powerful approaches for improvement of the genus Populus L. Pollination with artificial unreduced (2n) pollen was inefficiency, owing to weak competition of 2n pollen. To induce 2n megaspores and improve the efficiency of triploid production, female buds of Populus pseudo-simonii × Populus nigra ‘Zheyin3#’ were exposed to high temperature during megasporogenesis.

Results

A relationship between megasporogenesis and morphological changes of female buds was established to guide the high-temperature treatments. In the progeny, 146 triploids were obtained and the highest efficiency of triploid production was 66.7%. Both 41°C and 44°C were suitable for megaspore chromosome doubling. Cytological analysis showed that meiotic stages from pachytene to diplotene may be the optimal period for megaspore chromosome doubling at high temperature in the ‘Zheyin3#.’ Because megasporocytes both in the first meiotic division and the second division were treated, the first meiotic division restitution typed and the second meiotic division restitution typed 2n megaspores could be obtained.

Conclusion

Our findings indicated that hybridization with high-temperature-induced 2n megaspores is an ideal approach for triploid production. Offspring with different heterozygosity are valuable for genetic research and breeding programs of Populus.

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Acknowledgments

We thank the Forestry Research Institute of Tongliao City, the Inner Mongolia Autonomous Region, People’s Republic of China, for collecting the plant material and for additional help. This work was supported by the Fundamental Research Funds for the Central Universities (grant no. YX2010-17) and the National Natural Science Foundation of China (grant nos. 30671708 and 31000306).

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Authors and Affiliations

  1. National Engineering Laboratory for Tree Breeding, Beijing Forestry University, 100083, Beijing, People’s Republic of China

    Jun Wang & Xiang-Yang Kang

  2. Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, Beijing Forestry University, 100083, Beijing, People’s Republic of China

    Jun Wang, Dai-Li Li & Xiang-Yang Kang

  3. Beijing Forestry University, P. O. Box 118, NO.35, Tsinghua East Rd. Haidian District, 100083, Beijing, People’s Republic of China

    Jun Wang & Xiang-Yang Kang

Authors
  1. Jun Wang
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  2. Dai-Li Li
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  3. Xiang-Yang Kang
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Correspondence to Jun Wang or Xiang-Yang Kang.

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Handling Editor: Luc Paques

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Wang, J., Li, DL. & Kang, XY. Induction of unreduced megaspores with high temperature during megasporogenesis in Populus . Annals of Forest Science 69, 59–67 (2012). https://doi.org/10.1007/s13595-011-0152-5

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  • DOI: https://doi.org/10.1007/s13595-011-0152-5

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