Skip to main content

Advertisement

SpringerLink
Log in

Characterization of Fusarium head blight resistance in a CIMMYT synthetic-derived bread wheat line

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Fusarium head blight (FHB), also known as head scab, is a devastating fungal disease of bread and durum wheat worldwide. It reduces yield, lowers seed germination, reduces grain quality, and renders grain unsuitable for human or animal consumption due to mycotoxin contamination. Use of host resistance in commercially cultivated wheat varieties is an economical, effective and environmentally friendly method to manage FHB. In order to map loci underlying FHB resistance in synthetic-derived bread wheat line SYN1, a doubled haploid (DH) population of 169 lines was developed through hybridizing SYN1 with FHB-susceptible line Ocoroni. The DH population was evaluated in spray-inoculated field nurseries in the 2010 and 2011 cropping seasons at the El Batan Experiment Station of the International Maize and Wheat Improvement Center, Mexico. Ten marked spikes from each plot were scored for disease response and an FHB index was subsequently calculated. The population was genotyped with 1391 genotyping by sequencing and 106 simple-sequence repeat (SSR) loci and 28 linkage groups were constructed. These linkage groups were anchored by SSRs to all chromosomes, except 4D, 5D and 6D. Using the inclusive composite interval mapping algorithm, three genomic regions were associated with FHB resistance, including a major QTL on chromosome 2D accounting for 25 % of the phenotypic variation explained (PVE) and two minor QTLs on chromosomes 1B (4.7 % PVE) and 7A (4.2 % PVE), with all favorable alleles contributed by SYN1. The average FHB index of lines with all three SYN1 alleles was substantially lower (61.4 %) than that of those with Ocoroni alleles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Anderson JA, Chao S, Liu S (2007) Molecular breeding using a major QTL for Fusarium head blight resistance in wheat. Crop Sci 47:S112–S119

    Article  Google Scholar 

  • Bai G, Shaner G (1994) Scab of wheat: prospects for control. Plant Dis 78:760–766

    Article  Google Scholar 

  • Brennan JM, Egan D, Cooke BM, Doohan FM (2005) Effect of temperature on head blight of wheat caused by Fusarium culmorum and F. graminearum. Plant Pathol 54:156–160

    Article  Google Scholar 

  • Buerstmayr H, Lemmens M, Hartl L, Doldi L, Steiner B, Stierschneider M, Ruckenbauer P (2002) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (type II resistance). Theor Appl Genet 104:84–91

    Article  CAS  PubMed  Google Scholar 

  • Buerstmayr H, Ban T, Anderson JA (2009) QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: a review. Plant Breed 128:1–26

    Article  CAS  Google Scholar 

  • CIMMYT (2005) Laboratory protocols, 3rd edn. CIMMYT Applied Molecular Genetics Laboratory, Mexico, pp 1–10, 38–47

  • Dexter JE, Clear RM, Preston KR (1996) Fusarium head blight: effect on the milling and baking of some Canadian wheats. Cereal Chem 73:695–701

    CAS  Google Scholar 

  • Dexter JE, Marchylo BA, Clear RM, Clarke JM (1997) Effect of Fusarium head blight on semolina milling and pasta-making quality of durum wheat. Cereal Chem 74:519–525

    Article  CAS  Google Scholar 

  • Elshire RJ, Glaubitz JC, Sun Q, Poland JA, Kawamoto K, Buckler ES, Mitchell SE (2011) A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS ONE 6:e19379

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Ganal MW, Röder MS (2007) Microsatellite and SNP markers in wheat breeding. In: Varshney RK, Tuberosa R (eds) Genomics assisted crop improvement, vol 2: genomics applications in crops. Springer, New York, pp 1–24

    Chapter  Google Scholar 

  • He X, Singh PK, Duveiller E, Schlang N, Dreisigacker S, Singh RP (2013a) Identification and characterization of international Fusarium head blight screening nurseries of wheat at CIMMYT, Mexico. Eur J Plant Pathol 136:123–134

    Article  Google Scholar 

  • He X, Singh PK, Duveiller E, Dreisigacker S, Singh RP (2013b) Development and characterization of International Maize and Wheat Improvement Center (CIMMYT) germplasm for Fusarium head blight resistance. In: Alconada TM, Chulze SN (eds) Fusarium head blight in Latin America. Springer, Dordrecht, pp 241–262

    Chapter  Google Scholar 

  • He X, Singh PK, Schlang N, Duveiller E, Dreisigacker S, Payne T, He Z (2014) Characterization of Chinese wheat germplasm for resistance to Fusarium head blight at CIMMYT, Mexico. Euphytica 195:383–395

    Article  CAS  Google Scholar 

  • Ittu M, Saulescu NN, Hagima I, Ittu G, Mustatea P (2000) Association of Fusarium head blight resistance with gliadin loci in a winter wheat cross. Crop Sci 40:62–67

    Article  Google Scholar 

  • Jiang G, Huang D, Shen Q, Yang Z, Lu W, Shi J, Zhu H, Chen Z, Ward R (2006) Registration of wheat germplasms CJ W14 and CJ 9306 highly resistant to Fusarium head blight. Crop Sci 46:2326–2328

    Article  Google Scholar 

  • Jiang G, Dong Y, Shi J, Ward RW (2007a) QTL analysis of resistance to Fusarium head blight in the novel wheat germplasm CJ9306. II. Resistance to deoxynivalenol accumulation and grain yield loss. Theor Appl Genet 115:1043–1052

    Article  PubMed  Google Scholar 

  • Jiang G, Shi J, Ward RW (2007b) QTL analysis of resistance to Fusarium head blight in the novel wheat germplasm CJ9306. I. Resistance to fungal spread. Theor Appl Genet 116:3–13

    Article  CAS  PubMed  Google Scholar 

  • Knapp SJ, Stroup WW, Ross WM (1985) Exact confidence intervals for heritability on a progeny mean basis. Crop Sci 25:192–194

    Article  Google Scholar 

  • Lewis JM, Suenaga K, Ginkel MV, Gilchrist L, Shi JR, Jiang GL, Kravchenko S, Mujeeb-Kazi A, Ward RW (2004) Identification and mapping of a QTL for Type II resistance to Fusarium head blight on chromosome arm 2DL of wheat. In: Canty SM, Boring T, Versdahl K, Wardwell J, Ward RW (eds) Proceedings of 2nd international symposium on Fusarium head blight, Orlando, pp 89–92

  • Li T, Bai G, Wu S, Gu S (2011) Quantitative trait loci for resistance to Fusarium head blight in a Chinese wheat landrace Haiyanzhong. Theor Appl Genet 122:1497–1502

    Article  PubMed  Google Scholar 

  • Liu S, Anderson JA (2003) Marker assisted evaluation of Fusarium head blight resistant wheat germplasm. Crop Sci 43:760–766

    Article  CAS  Google Scholar 

  • Liu S, Hall MD, Griffey CA, McKendry AL (2009) Meta-analysis of QTL associated with Fusarium head blight resistance in wheat. Crop Sci 49:1955–1968

    Article  CAS  Google Scholar 

  • Lu Q, Lillemo M, Skinnes H, He X, Shi J, Ji F, Dong Y, Bjornstad A (2013) Anther extrusion and plant height are associated with Type I resistance to Fusarium head blight in bread wheat line ‘Shanghai-3/Catbird’. Theor Appl Genet 126:317–334

    Article  CAS  PubMed  Google Scholar 

  • McCartney CA, Somers DJ, Fedak G, DePauw RM, Thomas J, Fox SL, Humphreys DG, Lukow O, Savard ME, McCallum BD, Gilbert J, Cao W (2007) The evaluation of FHB resistance QTLs introgressed into elite Canadian spring wheat germplasm. Mol Breed 20:209–221

    Article  Google Scholar 

  • McMullen M, Jones R, Gallenberg D (1997) Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis 81:1340–1348

    Article  Google Scholar 

  • Mesterházy A (1995) Types and components of resistance to Fusarium head blight of wheat. Plant Breed 114:377–386

    Article  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Ogbonnaya FC, Abdalla O, Mujeeb-Kazi A, Kazi AG, Xu SS, Gosman N, Lagudah ES, Bonnett D, Sorrells ME, Tsujimoto H (2013) Synthetic hexaploids: harnessing species of the primary gene pool for wheat improvement. Plant Breed Rev 37:35–122

    Google Scholar 

  • Osman M, He X, Singh RP, Duveiller E, Lillemo M, Pereyra SA, Westerdijk-Hoks I, Kurushima M, Yau S-K, Benedettelli S, Singh PK (2015) Phenotypic and genotypic characterization of CIMMYT’s 15th international Fusarium head blight screening nursery of wheat. Euphytica 205:521–537

    Article  Google Scholar 

  • Pumphrey MO, Bernardo R, Anderson JA (2007) Validating the Fhb1 QTL for Fusarium head blight resistance in near-isogenic wheat lines developed from breeding populations. Crop Sci 47:200–206

    Article  CAS  Google Scholar 

  • Shen XR, Ittu M, Ohm HW (2003) Quantitative trait loci conditioning resistance to Fusarium head blight in wheat line F201R. Crop Sci 43:850–857

    Article  CAS  Google Scholar 

  • Somers DJ, Fedak G, Savard M (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat. Genome 46:555–564

    Article  CAS  PubMed  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  CAS  PubMed  Google Scholar 

  • Stack RW, McMullen MP (1994) A visual scale to estimate severity of Fusarium head blight in wheat. North Dakota State University Extension Service, p 1095

  • Van Ooijen JW (2006) JoinMap® 4, Software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, Wageningen

    Google Scholar 

  • Wang J, Li H, Zhang L, Meng L (2011) Users’ Manual of QTL IciMapping Version 3.2. The Quantitative Genetics Group, Institute of Crop Science, Chinese Academy of Agricultural Sciences (CAAS) and Crop Research Informatics Lab (CRIL), International Maize and Wheat Improvement Center (CIMMYT)

  • Wilde F, Korzun V, Ebmeyer E, Geiger HH, Miedaner T (2007) Comparison of phenotypic and marker-based selection for Fusarium head blight resistance and DON content in spring wheat. Mol Breed 19:357–370

    Article  CAS  Google Scholar 

  • Yu J, Bai G, Cai S, Dong Y, Ban T (2008) New Fusarium head blight-resistant sources from Asian wheat germplasm. Crop Sci 48:1090–1097

    Article  Google Scholar 

  • Zhang X, Zhou MP, Ren LJ, Bai GH, Ma HX, Scholten OE, Guo PG, Lu WZ (2004) Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai. Euphytica 139:59–64

    Article  CAS  Google Scholar 

  • Zhang X, Pan H, Bai G (2012) Quantitative trait loci responsible for Fusarium head blight resistance in Chinese landrace Baishanyuehuang. Theor Appl Genet 125:495–502

    Article  CAS  PubMed  Google Scholar 

  • Zhou WC, Kolb FL, Yu JB, Bai GH, Boze LK, Domier LL (2004) Molecular characterization of Fusarium head blight resistance in Wangshuibai with simple sequence repeat and amplified fragment length polymorphism markers. Genome 47:1137–1143

    Article  CAS  PubMed  Google Scholar 

  • Zhu Z, Bonnett D, Ellis M, Singh PK, Heslot N, Dreisigacker S, Gao C, Mujeeb-Kazi A (2014) Mapping resistance to spot blotch in a CIMMYT synthetic-derived bread wheat. Mol Breed 34:1215–1228

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The first author was financially supported by the CIMMYT Global Wheat Program, China Scholarship Council, National Natural Science Foundation of China (Grant No. 31301306), Natural Science Foundation of Hubei Province (Grant No. 2014CFB316) and China Agriculture Research System. GBS genotyping was supported by the Seeds of Discovery project funded by the Sustainable Modernization of Traditional Agriculture (MasAgro) project supported by the Government of Mexico. The authors thank Dr. Etienne Duveiller for advice on data collection and analysis, Drs. Dazhao Yu and Xiwen Cai for reviewing the manuscript, Drs. Xiang Wang and Caixia Lan for providing SSR primers, and Roman Delgado, Hugo Lopez, and Jesus Sanchez for technical assistance.

Author information

Authors and Affiliations

  1. Institute of Food Crops, Hubei Academy of Agricultural Sciences/Hubei Engineering and Technology Research Center of Wheat/Wheat Disease Biology Research Station for Central China, 3 Nanhu Avenue, Wuhan, 430064, China

    Zhanwang Zhu & Chunbao Gao

  2. International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico, D.F., Mexico

    Zhanwang Zhu, David Bonnett, Marc Ellis, Xinyao He, Nicolas Heslot, Susanne Dreisigacker & Pawan Singh

  3. Department of Plant Breeding and Genetics, Cornell University, 240 Emerson Hall, Ithaca, NY, 14853, USA

    Nicolas Heslot

  4. Bayer Crop Science, 6693 90th St. S, Sabin, MN, 56580, USA

    David Bonnett

Authors
  1. Zhanwang Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. David Bonnett
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marc Ellis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xinyao He
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nicolas Heslot
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Susanne Dreisigacker
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Chunbao Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Pawan Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Pawan Singh.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (XLSX 11 kb)

Supplementary material 2 (DOCX 885 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhu, Z., Bonnett, D., Ellis, M. et al. Characterization of Fusarium head blight resistance in a CIMMYT synthetic-derived bread wheat line. Euphytica 208, 367–375 (2016). https://doi.org/10.1007/s10681-015-1612-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-015-1612-z

Keywords

Search

Navigation