Abstract
Four pospiviroids, columnea latent viroid (CLVd), pepper chat fruit viroid (PCFVd), potato spindle tuber viroid (PSTVd) and tomato apical stunt viroid (TASVd), were mechanically inoculated on Capsicum annuum (pepper) cv. Yolo Wonder. Symptoms were different, though most pronounced for PCFVd, i.e. delay in flower and fruit onset, increase of plant height, and reduction of fruit size. For studying seed transmission, seeds with high loads of infectious viroid were sown at different time intervals after harvesting, i.e. 0, 2 and 77 days. No transmission from seed to seedlings was recorded in a total of 765 seedlings, irrespective viroid species and time interval. For PCFVd, the experiment was repeated in another pepper cultivar with the similar outcome for 179 seedlings. The results of this study are discussed in view of the role of infested seed lots in causing outbreaks in practice.
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References
Borst, A., Box, A. T. A., & Fluit, A. C. (2004). False-positive results and contamination in nucleic acid amplification assays: Suggestions for a prevent and destroy strategy. European Journal of Clinical Microbiology & Infectious Diseases, 23, 289–299.
Botermans, M., Van de Vossenberg, B. T. L. H., Verhoeven, J. T. J., Roenhorst, J. W., Hooftman, M., Dekter, R., & Meekes, E. T. M. (2013). Development and validation of a real-time RT-PCR assay for generic detection of pospiviroids. Journal of Virological Methods, 187, 43–50.
Di Serio, F., Li, S.-F., Pallás, V., Owens, R. A., Randles, J. W., Sano, T., Verhoeven, J. T. J., Vidalakis, G., & Flores, R. (2017). Viroid taxonomy. In A. Hadidi, R. Flores, J. W. Randles, & P. Palukaitis (Eds.), Viroids and satellites (pp. 135–146). London: Elsevier Academic Press.
EPPO (2020). PM 7/XXX Detection and identification of Pospiviroids. EPPO Bulletin (in preparation); https://www.eppo.int/RESOURCES/eppo_standards/pm7_diagnostics.
Grasmick, M. E., & Slack, S. A. (1985). Symptom expression enhanced and low concentrations of potato spindle tuber viroid amplified in tomato with high light intensity and temperature. Plant Disease, 69, 49–51.
Hammond, R. W. (2017a). Economic significance of viroids in vegetable and field crops. In A. Hadidi, R. Flores, J. W. Randles, & P. Palukaitis (Eds.), Viroids and satellites (pp. 5–13). London: Elsevier Academic Press.
Hammond, R. W. (2017b). Seed, pollen and insect transmission of viroids. In A. Hadidi, R. Flores, J. W. Randles, & P. Palukaitis (Eds.), Viroids and satellites (pp. 521–530). London: Elsevier Academic Press.
Kryczynski, S., Paduch-Cichal, E., & Skrzeczkowski, L. R. (1988). Transmission of three viroids through seed and pollen of tomato plants. Journal of Phytopathology, 121, 51–57.
Lebas, B. S. M., Clover, G. R. G., Ochoa-Corona, F. M., Elliott, D. R., Tang, Z., & Alexander, B. J. R. (2005). Distribution of potato spindle tuber viroid in New Zealand glasshouse crops of capsicum and tomato. Australasian Plant Pathology, 34, 129–133.
Mackie, A. E., Rodoni, B. C., Barbetti, M. J., McKirdy, S. J., & Jones, R. A. C. (2016). Potato spindle tuber viroid: Alternative host reservoirs and strain found in a remote subtropical irrigation area. European Journal of Plant Pathology, 145, 433–446.
Matsushita, Y., & Tsuda, S. (2016). Seed transmission of potato spindle tuber viroid, tomato chlorotic dwarf viroid, tomato apical stunt viroid, and Columnea latent viroid in horticultural plants. European Journal of Plant Pathology, 145, 1007–1011.
Naktuinbouw. (2017). Pepper pospiviroids protocol Naktuinbouw, SPN-V044
eV2.1, 30-10-2017. https://www.naktuinbouw.com/sites/default/files/SPN-V044e%20v2.1%20Pepper%20pospiviroids.pdf. Accessed 07-01-2019.Roenhorst, J. W., Butôt, R. P. T., Van der Heijden, K. A., Hooftman, M., & Van Zaayen, A. (2000). Detection of chrysanthemum stunt viroid and potato spindle tuber viroid by return-polyacrylamide gel electrophoresis. EPPO Bulletin, 30, 453–456.
Sänger, H. L., & Ramm, K. (1975). Radioactive labelling of viroid-RNA. In R. Markham, D. R. Davies, D. A. Hopwood, & R. W. Horne (Eds.), Modification of the information content of plant cells. (pp. 229–252). Amsterdam: Elsevier Publications.
Simmons, H. E., Ruchti, T. B., & Munkvold, G. P. (2015). Frequencies of seed infection and transmission to seedlings by potato spindle tuber viroid (a Pospiviroid) in tomato. Journal of Plant Pathology and Microbiology, 6(6), 275.
Verhoeven, J. T. J., Jansen, C. C. C., Roenhorst, J. W., Flores, R., & De la Pena, M. (2009). Pepper chat fruit viroid: Biological and molecular properties of a proposed new species of the genus Pospiviroid. Virus Research, 144, 209–214.
Verhoeven, J. T. J., Jansen, C. C. C., Botermans, M., & Roenhorst, J. W. (2010a). Epidemiological evidence that vegetatively propagated, solanaceous plant species act as sources of Potato spindle tuber viroid inoculum for tomato. Plant Pathology, 59, 3–12.
Verhoeven, J. T. J., Hüner, L., Virscek-Marn, M., Mavric Plesko, I., & Roenhorst, J. W. (2010b). Mechanical transmission of Potato spindle tuber viroid between plants of Brugmansia suaveoles, Solanum jasminoides and potatoes and tomatoes. European Journal of Plant Pathology, 128, 417–421.
Verhoeven, J. T. J., Botermans, M., Jansen, C. C. C., & Roenhorst, J. W. (2011). First report of Pepper chat fruit viroid in capsicum pepper in Canada. New Disease Reports, 23, 15.
Verhoeven, J. T. J., Voogd, J. G. B., Strik, N., & Roenhorst, J. W. (2016). First report of Potato spindle tuber viroid in vegetatively propagated plants of Capsicum annuum in the Netherlands. New Disease Reports, 34, 12.
Verhoeven, J. T. J., Hammond, R. W., & Stancanelli, G. (2017a). Economic significance of viroids in ornamental crops. In A. Hadidi, R. Flores, J. W. Randles, & P. Palukaitis (Eds.), Viroids and satellites (pp. 27–38). London: Elsevier Academic Press.
Verhoeven, J. T. J., Koenraadt, H. M. S., Westenberg, M., & Roenhorst, J. W. (2017b). Characterization of tomato apical stunt viroid isolated from a 24-year old seed lot of Capsicum annuum. Archives of Virology, 162, 1741–1744.
Yanagisawa, H., & Matsushita, Y. (2017). Host ranges and seed transmission of Tomato planta macho viroid and Pepper chat fruit viroid. European Journal of Plant Pathology, 149, 211–217.
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Verhoeven, J.T.J., Koenraadt, H.M.S., Jodlowska, A. et al. Pospiviroid infections in Capsicum annuum: disease symptoms and lack of seed transmission. Eur J Plant Pathol 156, 21–29 (2020). https://doi.org/10.1007/s10658-019-01849-1
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DOI: https://doi.org/10.1007/s10658-019-01849-1