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Are biosynthesized nanomaterials toxic for the environment? Effects of perlite and CuO/perlite nanoparticles on unicellular algae Haematococcus pluvialis

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Abstract

The properties of nanomaterials such as perlite nanoparticles and their increased application have raised concerns about their probable toxic impacts on the aquatic ecosystems and algae. Here, a novel biochemical synthesis and immobilization of CuO is reported on perlite nanoparticles (CuO/Per-NPs) and its toxic effect on alga has been compared with nanoperlites. This biosynthesis of CuO/Per-NPs performed using phytochemicals of Haematococcus pluvialis, Sargassum angustifolium, and walnut leaves in the aqueous extract. The structural, morphological, and colloidal properties of the as-synthesized nanoparticles have been confirmed by various methods. According to the obtained results, the morphology of the synthesized CuO/Per-NPs was spherical with sizes ranging from about 13 to 24 nm. Besides, the effects of Per-NPs and CuO/Per-NPs on unicellular algae H. pluvialis were studied. The changes in the amount of chlorophyll a, chlorophyll b, and Carotenoids in the presence of different concentrations of Per-NPs (25, 50, 100 mg/L) were more than CuO/Per-NPs. Also, decreased growth rate and efficiency of photosystem II confirmed the toxic effects of Per-NPs. However, the toxicity of CuO/Per-NPs appears to be lower than that of Per-NPs, which can be due to the changes in the surface and cationic charge of modified nanoperlit. These changes lead to a decrease in the interaction of nanoparticles with H. pluvilalis and a reduction in ROS production. Finally, the results of GC-MS used to evaluate volatile compounds, indicated an increase in the number of phenolic compounds in comparison to the control samples in 25, 50, and 100 mg/L treatments of nano-perlite.

Highlights

  • The toxicity of biosynthesized CuO/Per-NPs appears to be lower than that of Per-NPs which are used as superabsorbent of heavy metals in water environment.

  • Decreasing growth rate and photosystem II efficiency confirmed the toxic effects of Per-NPs on Alga Haematococcus pluvialis.

  • Lower toxicity effect of CuO/Per-NPs than Per-NPs can be due to the changes in the surface and cationic charge of modified nanoperlit.

  • The changes in the surface of modified nanoperlit (CuO/Per-NPs) may lead to a decrease in the interaction of nanoparticles with H. pluvilalis and a reduction in ROS production.

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Data availability

Data are available by contacting JR (jafar_razeghi@tabrizu.ac.ir) and SJ (jafarirad@tabrizu.ac.ir)

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Author contributions

All authors listed in the current study contributed to the experimental design and conduct, data analysis and interpretation of results, as well as authoring the paper.

Funding

This study was supported entirely by funds internal to the university (University of Tabriz).

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Authors and Affiliations

  1. Department of Plant Sciences, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

    Borna Ali Babazadeh, Jafar Razeghi & Rouhollah Motafakkerazad

  2. Research institute of bioscience and biotechnology, University of Tabriz, Tabriz, Iran

    Saeed Jafarirad

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  1. Borna Ali Babazadeh
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  2. Jafar Razeghi
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  3. Saeed Jafarirad
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  4. Rouhollah Motafakkerazad
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Ali Babazadeh, B., Razeghi, J., Jafarirad, S. et al. Are biosynthesized nanomaterials toxic for the environment? Effects of perlite and CuO/perlite nanoparticles on unicellular algae Haematococcus pluvialis. Ecotoxicology 30, 899–913 (2021). https://doi.org/10.1007/s10646-021-02406-5

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