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LncRNA FAM230B Promotes Gastric Cancer Growth and Metastasis by Regulating the miR-27a-5p/TOP2A Axis

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Abstract

Aim

Long non-coding RNAs serve as key components of competing endogenous RNA (ceRNA) networks that underlie tumorigenesis. We investigated the pathogenic roles of lncRNA FAM230B and its molecular mechanism in gastric cancer (GC).

Method

The levels of FAM230B expression in five gastric cancer cell lines and in human gastric mucosal cells were compared by quantitative RT-PCR. To analyze the function of FAM230B in GC, we overexpressed FAM230B in AGS cells, silenced FAM230B in MGC-803 cells, and tested the effect of FAM230B on tumor growth in nude mice. The interaction between miR-27a-5p and FAM230B was predicted by a bioinformatics analysis and then verified with a dual-luciferase reporter assay. We also further investigated the role and mechanism of FAM230B by forcing overexpression of miR-27a-5p in MGC-803 gastric cancer cells.

Results

We found that FAM230B was highly expressed in gastric cancer cell lines and mainly located in the cytoplasm. FAM230B overexpression promoted the proliferation, migration, and invasion of AGS cells and repressed their apoptosis; it also facilitated tumor growth in vivo. In contrast, FAM230B knockdown suppressed the proliferation, migration, and invasion of MGC0803 cells, but enhanced their apoptosis and inhibited tumor growth in vivo. MiR-27a-5p expression was suppressed by FAM230B overexpression in AGS cells. MiR-27a-5p inhibited the proliferation, migration, and invasion of gastric cancer cells, and promoted the apoptosis of gastric cancer cells by reducing TOP2A (topoisomerase 2 alpha) expression.

Conclusion

Our study showed that lncRNA FAM230B might function to promote GC. FAM230B functioned as a ceRNA by sponging miR-27a-5p and enhancing TOP2A expression.

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Acknowledgments

This study was supported by grants from the Social Science and Technology Development Major Project of Dongguan (NO. 2018507150241638), the Medical Scientific Research Foundation of Guangdong Province (Nos. B2018016, B2018243), and the Social Science and Technology Development General Project of Dongguan (Nos. 201950715024926, 2018507150241501).

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Authors and Affiliations

  1. Department of Clinical Laboratory, SSL Central Hospital of Dongguan City, Affiliated Dongguan Shilong People’s Hospital of Southern Medical University, 1 Xianglong Road, Dongguan City, 510080, Guangdong Province, China

    Yejia Cui, Rong Pu, Jinjun Ye, Wanchan Chen, Yelin Yao & Yingai He

  2. Department of Clinical Pharmacy, SSL Central Hospital of Dongguan City, Affiliated Dongguan Shilong People’s Hospital of Southern Medical University, Dongguan City, China

    Haohai Huang

  3. Department of Gynaecology and Obstetrics, SSL Central Hospital of Dongguan City, Affiliated Dongguan Shilong People’s Hospital of Southern Medical University, Dongguan City, China

    Dan Liao

  4. Department of Clinical Pathology, SSL Central Hospital of Dongguan City, Affiliated Dongguan Shilong People’s Hospital of Southern Medical University, Dongguan City, China

    Yufeng Yang

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  1. Yejia Cui
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Contributions

YJC and RP conceived and designed the studies that led to submission of this manuscript. JJY, HHH, and DL acquired the data. YFY, WCC, YLY, and YGH contributed reagents/materials/analysis tools. All authors approved the final version of the manuscript.

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Correspondence to Rong Pu.

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Cui, Y., Pu, R., Ye, J. et al. LncRNA FAM230B Promotes Gastric Cancer Growth and Metastasis by Regulating the miR-27a-5p/TOP2A Axis. Dig Dis Sci 66, 2637–2650 (2021). https://doi.org/10.1007/s10620-020-06581-z

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