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Development of Calbindin- and Calretinin-Immunopositive Neurons in the Enteric Ganglia of Rats

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Abstract

Calbindin D28 K (CB) and calretinin (CR) are the members of the EF-hand family of calcium-binding proteins that are expressed in neurons and nerve fibers of the enteric nervous system. CB and CR are expressed differentially in neuronal subpopulations throughout the central and peripheral nervous systems and their expression has been used to selectively target specific cell types and isolate neuronal networks. The present study presents an immunohistochemical analysis of CB and CR in the enteric ganglia of small intestine in rats of different ages (newborn, 10-day-old, 20-day-old, 30-day-old, 60-day-old, 1-year-old, and 2-year-old). The data obtained suggest a number of age-dependent changes in CB and CR expression in the myenteric and submucous plexuses. In the myenteric plexus, the lowest percentage of CB-immunoreactive (IR) and CR-IR neurons was observed at birth, after which the number of IR cells increased in the first 10 days of life. In the submucous plexus, CB-IR and CR-IR neurons were observed from 10-day-old onwards. The percentage of CR-IR and CB-IR neurons increased in the first 2 months and in the first 20 days, respectively. In all animals, the majority of the IR neurons colocalized CR and CB. From the moment of birth, the mean of the cross-sectional area of the CB-IR and CR-IR neuronal profiles was larger than that of CB- and CR-negative cells.

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Acknowledgements

This work was supported by the Russian Science Foundation, Grant 17-15-01015.

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Authors and Affiliations

  1. Department of Normal Physiology and Biophysics, Yaroslavl State Medical University, Revoliucionnaya 5, Yaroslavl, Russia, 150000

    Petr M. Masliukov & Konstantin Moiseev

  2. Department of Normal and Pathological Anatomy, Kabardino-Balkarian State University named after H.M. Berbekov, Nalchik, Russia

    Antonina F. Budnik

  3. St. Petersburg State University, St. Petersburg, Russia

    Alexandr D. Nozdrachev

  4. Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, Antwerp, Belgium

    Jean-Pierre Timmermans

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  1. Petr M. Masliukov
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Correspondence to Petr M. Masliukov.

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Masliukov, P.M., Moiseev, K., Budnik, A.F. et al. Development of Calbindin- and Calretinin-Immunopositive Neurons in the Enteric Ganglia of Rats. Cell Mol Neurobiol 37, 1257–1267 (2017). https://doi.org/10.1007/s10571-016-0457-x

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