Abstract
Deacetylated porous cellulose acetate microspheres (DPCA) modified with hydroxyapatite (HA) penetrated polydopamine (PDA) coating was prepared by a one-step in-situ method and investigated as the scaffold for bone tissue regeneration. The physical and chemical properties of different scaffolds were analyzed by Scanning electron microscopy, Transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction and X-ray photoelectron spectrum. Test results proved the unique three-dimensional porous structure, homogeneous PDA coating, dispersive HA crystals of the DPCA-PDA-HA microspheres (DPPH). The excellent attachment, adhesion and proliferation of MC3T3-E1 preosteoblast on DPPH were manifested by cell adhesion and biocompatibility assessment. In vitro mineralization also shown MC3T3-E1 was capable of differentiating osteogenically. Such satisfactory biomedical performance of DPPH was ascribed to the one-step introducing process contributing to a uniform and enrich growth of HA on the microspheres surface and interior. This study provides a promising cellulose-based scaffold applied in bone regeneration field.
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References
Atila D, Karataş A, Evcin A, Keskin D, Tezcaner A (2019) Bacterial cellulose-reinforced boron-doped hydroxyapatite/gelatin scaffolds for bone tissue engineering. Cellulose 26:9765–9785. https://doi.org/10.1007/s10570-019-02741-1
Chen JD, Pan PP, Zhang YJ, Zhong SN, Zhang QQ (2015) Preparation of chitosan/nano hydroxyapatite organic-inorganic hybrid microspheres for bone repair. Colloid Surf B 134:401–407. https://doi.org/10.1016/j.colsurfb.2015.06.072
Chen P, Liu LY, Pan JQ, Mei J, Li CR, Zheng YY (2019) Biomimetic composite scaffold of hydroxyapatite/gelatin-chitosan core-shell nanofibers for bone tissue engineering. Mat Sci Eng C-Mater 97:325–335. https://doi.org/10.1016/j.msec.2018.12.027
Fan XS, Liu ZW, Lu J, Liu ZT (2009) Cellulose triacetate optical film preparation from Ramie fiber. Ind Eng Chem Res 48:6212–6215. https://doi.org/10.1021/ie801703x
Farzadi A, Bakhshi F, Solati-Hashjin M, Asadi-Eydivand M, Abu Osman NA (2014) Magnesium incorporated hydroxyapatite: synthesis and structural properties characterization. Ceram Int 40:6021–6029. https://doi.org/10.1016/j.ceramint.2013.11.051
Fragal EH, Cellet TSP, Fragal VH, Witt MA, Companhoni MVP, Ueda-Nakamura T, Silva R, Rubira AF (2019) Biomimetic nanocomposite based on hydroxyapatite mineralization over chemically modified cellulose nanowhiskers: an active platform for osteoblast proliferation. Int J Biol Macromol 125:133–142. https://doi.org/10.1016/j.ijbiomac.2018.12.004
French AD (2014) Idealized powder diffraction patterns for cellulose polymorphs. Cellulose 21:885–896. https://doi.org/10.1007/s10570-013-0030-4
Fu LH, Liu YJ, Ma MG, Zhang XM, Xue ZM, Zhu JF (2016) Microwave-assisted hydrothermal synthesis of cellulose/hydroxyapatite nanocomposites. Polymers-Basel 8:316. https://doi.org/10.3390/polym8090316
Gouma P, Xue R, Goldbeck CP, Perrotta P, Balázsi C (2012) Nano-hydroxyapatite-Cellulose acetate composites for growing of bone cells. Mat Sci Eng C-Mater 32:607–612. https://doi.org/10.1016/j.msec.2011.12.019
Hammonds RL, Harrison MS, Cravanas TC, Gazzola WH, Stephens CP, Benson RS (2012) Biomimetic hydroxyapatite powder from a bacterial cellulose scaffold. Cellulose 19:1923–1932. https://doi.org/10.1007/s10570-012-9767-4
Jiang H, Zuo Y, Cheng L, Wang HL, Gu AQ, Li YB (2011) A homogenous CS/NaCMC/n-HA polyelectrolyte complex membrane prepared by gradual electrostatic assembling. J Mater Sci-Mater M 22:289–297. https://doi.org/10.1007/s10856-010-4195-1
Khoshroo K, Kashi TSJ, Mortarzadeh F, Tahriri M, Jazayeri HE, Tayebi L (2017) Development of 3D PCL microsphere/TiO2 nanotube composite scaffolds for bone tissue engineering. Mat Sci Eng C-Mater 70:586–598. https://doi.org/10.1016/j.msec.2016.08.081
Kumar A, Negi YS, Choudhary V, Bhardwaj NK (2014) Microstructural and mechanical properties of porous biocomposite scaffolds based on polyvinyl alcohol, nano-hydroxyapatite and cellulose nanocrystals. Cellulose 21:3409–3426. https://doi.org/10.1007/s10570-014-0339-7
Lee JH, Lee YJ, Cho HJ, Shin H (2014) Guidance of in vitro migration of human mesenchymal stem cells and in vivo guided bone regeneration using aligned electrospun fibers. Tissue Eng Pt A 20:2031–2042. https://doi.org/10.1089/ten.tea.2013.0282
Li XY, Ma BJ, Li JH, Shang LL, Liu H, Ge SH (2020) A method to visually observe the degradation-diffusion-reconstruction behavior of hydroxyapatite in the bone repair process. Acta Biomater 101:554–564. https://doi.org/10.1016/j.actbio.2019.10.044
Liu M, Jiang WQ, Chen Q, Wang S, Mao Y, Gong XL, Leung KCF, Tian J, Wang HJ, Xuan SH (2016) A facile one-step method to synthesize SiO2@polydopamine core-shell nanospheres for shear thickening fluid. Rsc Adv 6:29279–29287. https://doi.org/10.1039/C5RA25759J
Liu XY, Shen H, Song SJ, Chen W, Zhang ZJ (2017) Accelerated biomineralization of graphene oxide - incorporated cellulose acetate nanofibrous scaffolds for mesenchymal stem cell osteogenesis. Colloid Surface B 159:251–258. https://doi.org/10.1016/j.colsurfb.2017.07.078
Niamsap T, Lam NT, Sukyai P (2019) Production of hydroxyapatite-bacterial nanocellulose scaffold with assist of cellulose nanocrystals. Carbohyd Polym 205:159–166. https://doi.org/10.1016/j.carbpol.2018.10.034
Pandele AM, Comanici FE, Carp CA, Miculescu F, Voicu SI, Thakur VK, Serban BC (2017) Synthesis and characterization of cellulose acetate-hydroxyapatite micro and nano composites membranes for water purification and biomedical applications. Vacuum 146:599–605. https://doi.org/10.1016/j.vacuum.2017.05.008
Pandele AM, Neacsu P, Cimpean A, Staras AI, Miculescu F, Iordache A, Voicu SI, Thakur VK, Toader OD (2018) Cellulose acetate membranes functionalized with resveratrol by covalent immobilization for improved osseointegration. Appl Surf Sci 438:2–13. https://doi.org/10.1016/j.apsusc.2017.11.102
Pattinson SW, Hart AJ (2017) Additive manufacturing of cellulosic materials with robust mechanics and antimicrobial functionality. Adv Mater Technol-Us 2:1600084. https://doi.org/10.1002/admt.201600084
Peng S, Meng HC, Ouyang Y, Chang J (2014) Nanoporous magnetic cellulose-chitosan composite microspheres: preparation, characterization, and application for Cu (II) adsorption. Ind Eng Chem Res 53:2106–2113
Peng S, Gao F, Zeng D, Peng C, Chen YM, Li M (2018) Synthesis of Ag-Fe3O4 nanoparticles supported on polydopamine-functionalized porous cellulose acetate microspheres: catalytic and antibacterial applications. Cellulose 25:4771–4782. https://doi.org/10.1007/s10570-018-1886-0
Rim NG, Kim SJ, Shin YM, Jun I, Lim DW, Park JH, Shin H (2012) Mussel-inspired surface modification of poly(L-lactide) electrospun fibers for modulation of osteogenic differentiation of human mesenchymal stem cells. Colloid Surface B 91:189–197. https://doi.org/10.1016/j.colsurfb.2011.10.057
Ryu J, Ku SH, Lee H, Park CB (2010) Mussel-inspired polydopamine coating as a universal route to hydroxyapatite crystallization. Adv Funct Mater 20:2132–2139. https://doi.org/10.1002/adfm.200902347
Sarkar C, Chowdhuri AR, Garai S, Chakraborty J, Sahu SK (2019a) Three-dimensional cellulose-hydroxyapatite nanocomposite enriched with dexamethasone loaded metal-organic framework: a local drug delivery system for bone tissue engineering. Cellulose 26:7253–7269. https://doi.org/10.1007/s10570-019-02618-3
Sarkar C, Sahu SK, Sinha A, Chakraborty J, Garai S (2019b) Facile synthesis of carbon fiber reinforced polymer-hydroxyapatite ternary composite: a mechanically strong bioactive bone graft. Mat Sci Eng C-Mater 97:388–396. https://doi.org/10.1016/j.msec.2018.12.064
Seong YJ, Song EH, Park C, Lee H, Kang IG, Kim HE, Jeong SH (2020) Porous calcium phosphate-collagen composite microspheres for effective growth factor delivery and bone tissue regeneration. Mat Sci Eng C-Mater 109:110480. https://doi.org/10.1016/j.msec.2019.110480
Sun HY, Ai M, Zhu SQ, Jia XL, Cai Q, Yang XP (2015) Polylactide-hydroxyapatite nanocomposites with highly improved interfacial adhesion via mussel-inspired polydopamine surface modification. Rsc Adv 5:95631–95642. https://doi.org/10.1039/c5ra21010k
Thangavelu M, Adithan A, Peter JSJ, Hossain MA, Kim NS, Hwang KC, Khang G, Kim JH (2020) Ginseng compound K incorporated porous Chitosan/biphasic calcium phosphate composite microsphere for bone regeneration. Int J Biol Macromol 146:1024–1029. https://doi.org/10.1016/j.ijbiomac.2019.09.228
Tu H, Zhu MX, Duan B, Zhang LN (2020) Recent progress in high-strength and robust regenerated cellulose materials. Adv Mater. https://doi.org/10.1002/adma.202000682
Valtanen RS, Yang YP, Gurtner GC, Maloney WJ, Lowenberg DW (2020) Synthetic bone tissue engineering graft substitutes: What is the future? Injury. https://doi.org/10.1016/j.injury.2020.07.040
Wang JH, Sun XM, Zhang ZH, Wang YY, Huang CG, Yang CR, Liu LR, Zhang QQ (2019) Silk fibroin/collagen/hyaluronic acid scaffold incorporating pilose antler polypeptides microspheres for cartilage tissue engineering. Mat Sci Eng C-Mater 94:35–44. https://doi.org/10.1016/j.msec.2018.09.017
Yamaguchi K, Prabakaran M, Ke M, Gang X, Chung IM, Um IC, Gopiraman M, Kim IS (2016) Highly dispersed nanoscale hydroxyapatite on cellulose nanofibers for bone regeneration. Mater Lett 168:56–61. https://doi.org/10.1016/j.matlet.2016.01.010
Yuan ZY, Wei PF, Huang YQ, Zhang WX, Chen FY, Zhang X, Mao JP, Chen DF, Cai Q, Yang XP (2019) Injectable PLGA microspheres with tunable magnesium ion release for promoting bone regeneration. Acta Biomater 85:294–309. https://doi.org/10.1016/j.actbio.2018.12.017
Zhang D, Xu W, Cai J, Chen SY, Ding WP (2020) Citric acid-incorporated cellulose nanofibrous mats as food materials-based biosorbent for removal of hexavalent chromium from aqueous solutions. Int J Biol Macromol 149:459–466. https://doi.org/10.1016/j.ijbiomac.2020.01.199
Zhou YZ, Cao Y, Liu W, Chu CH, Li QL (2012) Polydopamine-induced tooth remineralization. Acs Appl Mater Inter 4:6901–6910. https://doi.org/10.1021/am302041b
Acknowledgments
This work was supported by the Foundation of State Key Laboratory of Coal Combustion (FSKLCCA2109), Major Technological Innovation of Hubei Province of China (2018ABA093), Science and Technology Research Project of Education Department of Hubei Province (Q20181711) and Wuhan Municipal Science and Technology Bureau (2017050304010277, 2017050304010277).
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Gao, F., Zeng, D., Liu, H. et al. Porous cellulose microspheres coated in one step with a polydopamine suspension of hydroxyapatite for bone tissue engineering. Cellulose 29, 1955–1967 (2022). https://doi.org/10.1007/s10570-021-04395-4
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DOI: https://doi.org/10.1007/s10570-021-04395-4