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Lipase Cocktail for Optimized Biodiesel Production of Free Fatty Acids from Residual Chicken Oil

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Abstract

In this work, the combination of different lipases was employed for the production of biodiesel, using the Free Fatty Acids (FFAs) from Residual Chicken Oil (RCO) as a substrate. As biocatalysts, lipases A and B from Candida antarctica (CALA and CALB, respectively) and lipase from Rhizomucor miehei (RML) were used in different combinations; as a result, the best lipase cocktail was composed of 67% CALB and 33% RML, which was used to optimize the esterification reaction parameters (molar ratio (FFAs/ ethyl alcohol), biocatalyst content, temperature and time) by the Taguchi method. Under optimized conditions (1:5, 15% of lipase cocktail, 30 °C and 3 h), it was possible to achieve 89.95 ± 0.3% of conversion.

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Acknowledgments

We gratefully acknowledge the financial support of Brazilian Agencies for Scientific and Technological Development, Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP), Project Number BP3-0139-00005.01.00/18, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Project Numbers 422942/2016-2, 408790/2016-4 and 311062/2019-9. Coordenação de Aperfeiçoamento de Ensino Superior (CAPES). In addition, we gratefully acknowledge the supply of Lipases by Mr. Martinez (Novozymes, Spain).

Funding

Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP), Project Number BP3-0139-00005.01.00/18, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Project Numbers 422942/2016-2 and 408790/2016-4, Coordenação de Aperfeiçoamento de Ensino Superior (CAPES).

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  1. Instituto de Engenharias E Desenvolvimento Sustentável, Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Campus das Auroras, Redenção, CE, CEP 62790970, Brazil

    Thales G. Rocha, Pedro H. de L. Gomes, Maria C. M. de Souza & José C. S. dos Santos

  2. Departamento de Engenharia Química, Universidade Federal Do Ceará, Campus do Pici, Bloco 709, Fortaleza, CE, CEP 60455760, Brazil

    Rodolpho R. C. Monteiro & José C. S. dos Santos

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  2. Pedro H. de L. Gomes
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  4. Rodolpho R. C. Monteiro
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Contributions

Conceptualization, TGR, PHDLG; Formal analysis, TGR, PHDLG, and RRCM; Methodology, MCMS and JCSDS; Project administration, JCSDS; Resources, TGR, PHDLG and MCMDS; Supervision, MCMDS and JCSDS; Writing–original draft, JCSDS; Writing–review & editing, TGR, PHDLG, RRCM, MCMDS and JCSDS.

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Rocha, T.G., de L. Gomes, P.H., de Souza, M.C.M. et al. Lipase Cocktail for Optimized Biodiesel Production of Free Fatty Acids from Residual Chicken Oil. Catal Lett 151, 1155–1166 (2021). https://doi.org/10.1007/s10562-020-03367-w

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