Abstract
In plants, class III T2 RNases involves two groups of structurally similar proteins, but with different biological functions: S-RNases and non-S-RNases. S-RNases have been involved in self-incompatibility whereas non-S-RNases have been implicated in stress responses. Here we report a novel class III RNase termed NaPi/Sx-RNase, which works both in self-incompatibility and in response to phosphate deficiency. The NaPi/Sx-RNase gene was identified in roots of Nicotiana alata grown in the absence of inorganic phosphate. Phylogenetic analysis showed that NaPi/Sx-RNase was included within the class III RNase T2 group. The NaPi/Sx-RNase was expressed in styles and its temporal expression increased in parallel to stylar development, with a slight decrease after anthesis. Progeny analysis showed that NaPi/Sx-RNase and S107-RNase, a functional allele of the self-incompatibility system, segregated in a 1:1 ratio. The progeny segregation of a semicompatible cross, in which NaPi/Sx-RNase was shared by the two parents, exhibited a pattern consistent with a functional S-RNase allele. Considering genetic segregation, primary structure, and physiological role, the NaPi/Sx-RNase may be either an S-RNase with diversified functions or a non-S-RNase linked to the S-locus. To our knowledge, this is the first evidence for a specific function of the S-locus other than the self-incompatibility reaction. These results support the hypothesis that the self-incompatibility and stress responses may have evolved from a common origin.
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Abbreviations
- Na:
-
Nicotiana alata
- Pi:
-
inorganic phosphate
- RNase:
-
ribonuclease
- RT-PCR:
-
reverse transcription polymerase chain reaction
- SI:
-
self-incompatibility
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Acknowledgements: We thank Gabriela Diaz Cortez for editorial and language assistance. This work was supported by the Agencia Nacional de Promoción Científica y Tecnológica (PICT 12/1322), and the Secretaría de Ciencia y Técnica (SECyT) of Universidad Nacional de Córdoba (366/16).
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Rojas, H.J., Caspani, C., Escobar, E.G. et al. NaPi/SX-RNase segregates as a functional S-RNase and is induced under phosphate deficiency in Nicotiana alata. Biol Plant 62, 261–268 (2018). https://doi.org/10.1007/s10535-018-0783-6
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DOI: https://doi.org/10.1007/s10535-018-0783-6