Abstract
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) serves not only as a key enzyme in glycolysis, but also as a multifunctional protein in other biological processes, especially in response to abiotic stresses in plants. Cytosolic GAPDH (GAPC) is a typical redox protein with selected catalytic cysteine, which undergoes reversible redox post-translational modifications (RPTMs) on its thiol group by reacting with hydrogen peroxide and nitric oxide related species. Moreover, the modified GAPC may interact with certain signal transmitters such as phosphatidic acid, phospholipase D, and osmotic stress-activated protein kinase. All these observations suggest that GAPC serve as a key mediator in redox signal transduction in plants. In this review, we provide an up-to-date insight into molecular mechanisms after H2O2- and NO-dependent oxidation of GAPC. We also discuss GAPC catalytic functions and potential functions as a modified protein by RPTMs.
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Abbreviations
- ABA:
-
abscisic acid
- ATG3:
-
autophagy-related protein
- BaMV:
-
bamboo mosaic virus
- BPGA:
-
1,3-bisphosphoglyceric acid
- DEA-NO:
-
diethylamine NONOate
- DTT:
-
dithiothreitol
- FER:
-
FERONIA
- GAPC:
-
cytosolic GAPDH
- GAPDH:
-
glyceraldehyde-3-phosphate dehydrogenase
- G3P:
-
glyceraldehyde-3-phosphate
- Grx:
-
glutaredoxins
- GSNO:
-
nitrosoglutathione
- MAP:
-
mitogen-activated protein
- NtOSAK:
-
Nicotiana tabacum osmotic stress-activated protein kinase
- OPP:
-
oxidative pentose phosphate
- OXI1:
-
oxidative signal-inducible1
- PA:
-
phosphatidic acid
- PCD:
-
programmed cell death
- PLD:
-
phospholipase D
- QM:
-
quantum mechanics
- RNS:
-
reactive nitrogen species
- RPTM:
-
redox post-translational modification
- ROS:
-
reactive oxygen species
- SINAL7:
-
SEVEN IN ABSENTIA like7
- SnRK2:
-
SNF1 (sucrose non-fermenting 1)-related protein kinase 2
- SOH:
-
sulphenylation
- SSG:
-
glutathionylation
- Trx:
-
thioredoxins
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Acknowledgments: We are greatly acknowledged to the National Natural Science Foundation of China (Nos. 31271625 and 31671609), the State Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Sciences (No. 10502), the National Basic Research Program of China (2015CB150402), and the National Natural Science Foundation of China (No. 51479189).
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Yang, S.S., Zhai, Q.H. Cytosolic GAPDH: a key mediator in redox signal transduction in plants. Biol Plant 61, 417–426 (2017). https://doi.org/10.1007/s10535-017-0706-y
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DOI: https://doi.org/10.1007/s10535-017-0706-y