Abstract
At currently, approximately 70 species of magnetotactic bacteria have been identified; thus, there is an urgent need to identify more magnetotactic bacteria from diverse environmental sources with potential applications in industry and biotechnology. To the best of our knowledge, this is the first magnetotactic bacterial strain discovered in Pakistan. The first magnetotactic bacteria, Magnetospirillum moscoviense MS-24, was isolated from Banjosa Lake (Rawalakot), Pakistan, in the current investigation. Magnetospirillum moscoviense MS-24 was screened using the Racetrack method. The Magnetospirillum moscoviense MS-24 were physically characterised using Atomic Force Microscopy, High-Resolution Scanning Electron Microscopy, and Transmission Electron Microscopy. The current study used microscopy to illustrate the shape of bacteria and to find a very obvious chain of magnetosomes within the bacterial cell. The Magnetospirillum moscoviense MS-24 measured about 4 ± 0.04 µm in length and 600 ± 0.02 nm in diameter. The microfluidic chip experiments were also used to detect magnetotaxis behaviour in bacteria.
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Acknowledgements
We acknowledge Prof. Dr. Leon Abelmann (Department of Robotics and Mechatronics, University of Twente, Netherlands) for his support and hospitality. This study was partially supported by the Higher Education Commission of Pakistan through International Research Support Initiative Program.
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This research was partially funded by Higher Education Commission (HEC) of Pakistan through International Research Support Initiative Program (IRSIP).
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Conceptualization: MAS; Data curation: MAS; Experimental design: MAS and FH; Formal analysis: MAS and NK (HR-SEM analysis); Writing–original draft: MAS; Review & editing: FH, NK and LMC. FH provided experimental research guidelines.
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Salam, M.A., Korkmaz, N., Cycil, L.M. et al. Isolation, microscopic and magnetotactic characterization of Magnetospirillum moscoviense MS-24 from Banjosa Lake, Pakistan. Biotechnol Lett 45, 967–979 (2023). https://doi.org/10.1007/s10529-023-03390-y
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DOI: https://doi.org/10.1007/s10529-023-03390-y