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Production of 7,10,12-trihydroxy-8(E)-octadecenoic acid from ricinoleic acid by Pseudomonas aeruginosa KNU-2B

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Abstract

Microbial production of hydroxy fatty acids (HFAs) was widely studied because of important biological properties of HFAs. Among microorganisms producing HFAs, Pseudomonas aeruginosa PR3 was well known to produce various HFAs from different unsaturated fatty acids. Recently, a new variant species of P. aeruginosa PR3 was isolated and characterized, showing improved efficiency for producing 7,10-dihydroxy-8(E)-octadecenoic acid from oleic acid. In this study, we report the production of 7,10,12-trihydroxy-8(E)-octadecenoic acid (TOD) from ricinoleic acid by the newly isolated P. aeruginosa KNU-2B. TOD was efficiently produced from ricinoleic acid by KNU-2B with the maximum conversion yield of 56.7% under the optimum reaction conditions of pH 8.0 and 48-h incubation at 27 °C, 150 rpm. Under optimized reaction conditions, maximum TOD production reached 340.3 mg/100 mL of the culture. However, requirement of nutritional factors by KNU-2B for production of TOD were considerably different from those by PR3 strain.

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Acknowledgements

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2018R1D1A3B07040443).

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Authors and Affiliations

  1. School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, Korea

    Yeon-Jung Lee, Ji-Sun Moon & Hak-Ryul Kim

  2. Department of Public Health Sciences, Graduate School, Korea University, Seoul, Korea

    In-Hwan Kim

  3. Institute of Agricultural Science & Technology, Kyungpook National University, Daegu, Korea

    Hak-Ryul Kim

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  1. Yeon-Jung Lee
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  2. Ji-Sun Moon
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  4. Hak-Ryul Kim
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Correspondence to Hak-Ryul Kim.

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Lee, YJ., Moon, JS., Kim, IH. et al. Production of 7,10,12-trihydroxy-8(E)-octadecenoic acid from ricinoleic acid by Pseudomonas aeruginosa KNU-2B. Biotechnol Lett 42, 1547–1558 (2020). https://doi.org/10.1007/s10529-020-02883-4

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