Skip to main content
SpringerLink
Log in

Environmental optimization for bioconversion of triolein into 7,10-dihydroxy-8(E)-octadecenoic acid by Pseudomonas aeruginosa PR3

  • Biotechnological Products and Process Engineering
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Hydroxy fatty acids (HFAs), originally found in small amount mainly from plant systems, are well known to have special properties such as higher viscosity and reactivity compared with other normal fatty acids. Recently, various microbial strains were tested to produce HFAs from different unsaturated fatty acids. Among those microbial strains tested, Pseudomonas aeruginosa PR3 are well known to utilize various unsaturated fatty acids to produce mono-, di-, and tri-HFAs. Previously, we reported that strain PR3 could utilize triolein as a substrate for the production of 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) via the induction of lipase activity (Chang et al., Appl Microbiol Biotechnol, 74:301–306, 2007). In this study, we focused on the development of the optimal environmental conditions for DOD production from triolein by PR3. Optimal initial medium pH and incubation temperature were pH 8.0 and 25°C, respectively. Magnesium ion was essentially required for DOD production. Optimal inoculum size, time for substrate addition, and substrate concentration were 1%, 12 to 24 h, and 300 mg, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Bae JH, Kim DS, Suh MJ, Oh SR, Lee IJ, Kang SC, Hou CT, Kim HR (2007) Production and identification of 7, 10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa PR3. Appl Microbiol Biotechnol 75:435–440

    Article  CAS  Google Scholar 

  • Bagby MO, Calson KD (1989) Chemical and biological conversion of soybean oil for industrial products. In: Cambie RC (ed) Fats for the future. Ellis Horwood, Chichester, UK, pp 301–317

    Google Scholar 

  • Bajpai V, Shin SY, Kim MJ, Kim HR, Kang SC (2004) Antifungal activity of bioconverted oil extract of linoleic acid and fractionated dilutions against phytopathogens Rhizoctonia solani and Botrytis cinerea. Agric Chem Biotechnol 47:199–204

    CAS  Google Scholar 

  • Chang IA, Kim IH, Kang SC, Hou CT, Kim HR (2007) Production of 7,10-dihydroxy-8(E)-octadecenoic acid from triolein via lipase induction by Pseudomonas aeruginosa PR3. Appl Microbiol Biotechnol 74:301–306

    Article  CAS  Google Scholar 

  • Gardner HW, Weisleder D, Kleiman R (1976) Formation of trans-12,13-epoxy-9-hydroperoxy-trans-10-octadecenoic acid from 13-L-hydroperoxy-cis-9, trans-11-octadecenoic acid catalyzed by either a soybean extract or cystein-FeCl3. Lipids 13:246–52

    Article  Google Scholar 

  • Hou CT, Bagby MO (1992) 10-Hydroxy-8(Z)-octadecenoic acid, an Intermediate in the bioconversion of oleic acid to 7,10-dihydroxy-8(E)-octadecenoic acid. J Ind Microbiol 9:103–107

    Article  CAS  Google Scholar 

  • Hou CT, Forman RJ (2000) Growth inhibition of plant pathogenic fungi by hydroxy fatty acids. J Ind Microbiol Biotechnol 24:275–276

    Article  CAS  Google Scholar 

  • Hou CT, Bagby MO, Plattner RD, Koritala S (1991) A novel compound, 7,10-dihydroxy-8(E)-octadecenoic acid from oleic acid by bioconversion. J Am Oil Chem Soc 68:99–101

    Article  CAS  Google Scholar 

  • Kato T, Yamaguchi Y, Abe N, Uyehara T, Nakai T, Yamanaka S, Harada N (1984) Unsaturated hydroxy fatty acids, the self-defensive substances in rice plant against rice blast disease. Chem Lett 25:409–412

    Article  Google Scholar 

  • Kim H, Gardner HW, Hou CT (2000a) Production of isomeric (9,10,13)-trihydroxy-11E(10E)-octadecenoic acid from linoleic acid by Pseudomonas aeruginosa PR3. J Ind Microbiol Biotechnol 25:109–115

    Article  CAS  Google Scholar 

  • Kim H, Gardner HW, Hou CT (2000b) 10(S)-Hydroxy-8(E)-octadecenoic acid, an intermediate in the conversion of oleic acid to 7,10-dihydroxy-8(E)-octadecenoic acid. J Am Oil Chem Soc 77:95–99

    Article  CAS  Google Scholar 

  • Kuo TM, Manthey LK, Hou CT (1998) Fatty acid bioconversion by Pseudomonas aeruginosa PR3. J Am Oil Chem Soc 75:875–879

    Article  CAS  Google Scholar 

  • Kuo TM, Kim H, Hou CT (2001) Production of a novel compound, 7,10,12-trihydroxy-8(E)-octadecenoic acid from ricinoleic acid by Pseudomonas aeruginosa PR3. Curr Microbiol 43:198–203

    Article  CAS  Google Scholar 

  • Shin SY, Kim HR, Kang SC (2004) Antibacterial activity of various hydroxy fatty acids bioconverted by Pseudomonas aeruginosa PR3. Agric Chem Biotechnol 47:205–208

    CAS  Google Scholar 

  • Zimmerman DC (1966) A new product of linoleic acid oxidation by a flaxseed enzyme. Biochem Biophys Res Commun 23:398–402

    Article  CAS  Google Scholar 

Download references

Acknowledgment

This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD; KRF-2006-521-F00065).

Author information

Authors and Affiliations

  1. Department of Animal Science and Biotechnology, Kyungpook National University, Daegu, South Korea, 702-701

    In-Ae Chang, Jae-Han Bae, Min-Jung Suh & Hak-Ryul Kim

  2. Department of Food and Nutrition, College of Health Science, Korea University, Seoul, South Korea

    In-Hwan Kim

  3. Microbial Genomic and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, Peoria, IL, USA

    Ching T. Hou

Authors
  1. In-Ae Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jae-Han Bae
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Min-Jung Suh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. In-Hwan Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ching T. Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hak-Ryul Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hak-Ryul Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chang, IA., Bae, JH., Suh, MJ. et al. Environmental optimization for bioconversion of triolein into 7,10-dihydroxy-8(E)-octadecenoic acid by Pseudomonas aeruginosa PR3. Appl Microbiol Biotechnol 78, 581–586 (2008). https://doi.org/10.1007/s00253-007-1342-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-007-1342-9

Keywords

Search

Navigation