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Downregulation of lncRNA NEAT1 Relieves Caerulein-Induced Cell Apoptosis and Inflammatory Injury in AR42J Cells Through Sponging miR-365a-3p in Acute Pancreatitis

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Abstract

Mounting evidence suggests that long non-coding RNAs (lncRNAs) and microRNAs exert a critical regulatory role in acute pancreatitis. The present study aimed to explore the role of lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) in acute pancreatitis (AP) that was induced by caerulein in rat pancreatic acinar cells (AR42J). The potential target sites of lncRNA NEAT1 and miR-365a-3p were predicted using starBase and were confirmed using dual-luciferase reporter assay. Reverse transcription-quantitative polymerase chain reaction was performed to assess lncRNA NEAT1 and miR-365a-3p expression levels in AP induced by caerulein. Cell Counting Kit-8 and flow cytometry assays were performed to assess AR42J cell viability. Western blotting was performed to evaluate the expression of apoptosis-related proteins. Interleukin (IL)-1β, IL-6, and tumor necrosis factor-α levels were detected by ELISA. The results of the dual-luciferase reporter assay confirmed that miR-365a-3p could bind to NEAT1. LncRNA NEAT1 was upregulated in AR42J cells treated with 10 nmol/l caerulein, and miR-365a-3p was expressed at low levels in an AP model. Overexpression of miR-365a-3p suppressed the apoptosis and inflammatory response of AR42J cells induced by caerulein. Importantly, inhibition of lncRNA NEAT1 decreased apoptosis and inflammation in caerulein-treated AR42J cells, while these effects were reverted upon co-transfection with a miR-365a-3p inhibitor. In conclusion, lncRNA NEAT1 was involved in AP progression by sponging miR-365a-3p and may thus be a novel target for treating patients with AP.

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Data Availability

The datasets used and/or analyzed during the present study are available from the corresponding author upon reasonable request.

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Authors and Affiliations

  1. Department of Gastroenterology, Chongqing Wanzhou Shanghai Hospital, Chongqing, 404100, People’s Republic of China

    Anjing Shao

  2. Department of Gastroenterology, Chongqing Jiulongpo District People’s Hospital, 7 Metallurgical Third Village, Shipingqiao, Jiulongpo, Chongqing, 400000, People’s Republic of China

    Wei Hu

  3. Department of Gastroenterology, Army Medical Center of PLA, Chongqing, 400042, People’s Republic of China

    Chunxia Li, Yang Yang & Jianru Zhu

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  1. Anjing Shao
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  2. Wei Hu
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  3. Chunxia Li
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  4. Yang Yang
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  5. Jianru Zhu
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Correspondence to Wei Hu.

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Shao, A., Hu, W., Li, C. et al. Downregulation of lncRNA NEAT1 Relieves Caerulein-Induced Cell Apoptosis and Inflammatory Injury in AR42J Cells Through Sponging miR-365a-3p in Acute Pancreatitis. Biochem Genet 60, 2286–2298 (2022). https://doi.org/10.1007/s10528-022-10219-2

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  • DOI: https://doi.org/10.1007/s10528-022-10219-2

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