We studied the effect of KE and AED peptides on the expression of sirtuin-1, sirtuin-6, collagen I, cytokines (IL-1, TGF-β), and transcription factor NF-κB in human skin fibroblasts during their replicative aging. Immunocytochemical analysis and confocal microscopy showed that KE peptide reduces the synthesis of factors of the inflammatory response IL-1, NF-κB, and TGF-β and stimulates the synthesis of sirtuin-6. KE peptide normalizes the immunological function of human skin fibroblasts during their aging. AED peptide activates the synthesis of sirtuin-1, sirtuin-6, and collagen I in human skin fibroblasts during their replicative aging, which attests to its geroprotective effect.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 197-201, September, 2020
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Fridman, N.V., Linkova, N.S., Kozhevnikova, E.O. et al. Comparison of the Effects of KE and AED Peptides on Functional Activity of Human Skin Fibroblasts during Their Replicative Aging. Bull Exp Biol Med 170, 154–157 (2020). https://doi.org/10.1007/s10517-020-05022-1
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DOI: https://doi.org/10.1007/s10517-020-05022-1