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Comparison of the Effect of Platinum (IV) Complexes on Spheroids and Monolayer Culture of HeLa Cells

  • Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

We performed a comparative study of the cytotoxicity of cisplatin, JM216 complex, and aminonitroxyl platinum(IV) complexes for HeLa cells grown in monolayer and 3D culture. The growth dynamics of spheroids was studied and optimal conditions for evaluation of cytotoxicity were determined. Spheroids were less sensitive to the test compounds than cells in a monolayer. The resistance index (RI) of spheroids was determined as the ratio of IC50 for spheroids to IC50 for monolayer culture. Resistance index was 5.0±1.5 for cisplatin and ranged from 1.8 to 2.3 for platinum(IV) complexes. The observed differences are related to different physicochemical properties of the complexes and different mechanisms of their penetration into cells.

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Authors and Affiliations

  1. Institute of Problems of Chemical Physics, Russian Academy of Science, Chernogolovka, Moscow, Russia

    М. М. Trigub, N. V. Filatova, D. А. Areshidze, V. D. Sen’ & А. А. Terentiev

  2. Scientific and Educational Center of the Moscow State Regional University in Chernogolovka, Mytishchi, Moscow, Russia

    М. М. Trigub, N. V. Filatova, D. А. Areshidze & А. А. Terentiev

  3. M. V. Lomonosov Moscow State University, Moscow, Russia

    D. А. Areshidze & А. А. Terentiev

Authors
  1. М. М. Trigub
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  2. N. V. Filatova
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  3. D. А. Areshidze
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  4. V. D. Sen’
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  5. А. А. Terentiev
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Correspondence to М. М. Trigub.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 273-279, December, 2019

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Trigub, М.М., Filatova, N.V., Areshidze, D.А. et al. Comparison of the Effect of Platinum (IV) Complexes on Spheroids and Monolayer Culture of HeLa Cells. Bull Exp Biol Med 168, 583–588 (2020). https://doi.org/10.1007/s10517-020-04757-1

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  • DOI: https://doi.org/10.1007/s10517-020-04757-1

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